A549 cell
DIC microscopy, from a 3-4 days old culture, showing an abundance of intercellular connections, including possible cytonemes, filopodia and other epithelial bridges. (These cells have endocytosed 25x73 nm colloidal gold
nanorods.)A549 cells are
explanted tumor of a 58-year-old caucasian male.[1] The cells are used as models for the study of lung cancer and the development of drug therapies against it.[2][3]
Characteristics
A549 cells, as found in the lung tissue of their origin, are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across
membrane phospholipids.[1] A549 cells are widely used as a type II pulmonary epithelial cell model for drug metabolism and as a transfection host.[4] When grown for a sufficiently long time in cell culture, A549 cells may begin to differentiate, as signaled by the presence of multilamellar bodies.[5]
Usage
A549 cells have served as models of
xenografting, respectively.[6][1] Single-cell tracking of A549 has enabled the elaboration of pedigree-tree profiles and demonstrate correlations in behavior among sister cells and their descendants.[7][8] Such observations of correlations can be used as proxy measurements to identify cellular stress and inheritance as a response to drug treatment.[9] A549 has also been employed in viral research and associated protein expression changes as a consequence of viral infection.[10] Although A549 is a cancer cell line, it has also been studied for its response to tuberculosis, specifically the production of chemokines as it is induced by the invading bacteria.[11]
References
- ^ a b c d "A549 Cell Line: Human alveolar adenocarcinoma cell line -General Information". Retrieved 3 January 2012.
- PMID 4357758.
- ^ PMID 9743595.
- ^ ATCC.org. "A549 cell line: CCl-185 Product Description". Archived from the original on 11 February 2012. Retrieved 3 January 2012.
- ^ Cooper, Jim (December 2012). "Cell line profile A549" (PDF). Public Health England. Retrieved 25 June 2018.
- ^ Franklin, Maryland (May 2016). "A549 – A Model For Non-Small Cell Lung Cancer". MiBioresearch. Archived from the original on 25 June 2018. Retrieved 25 June 2018.
- PMID 30023341.
- PMID 38090384.
- PMID 31678863.
- S2CID 83679578.
- PMID 9488404.
External links
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