Aldolase B
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Aldolase B also known as fructose-bisphosphate aldolase B or liver-type aldolase is one of three
In humans, aldolase B is encoded by the ALDOB
Mechanism
The generic fructose bisphosphate aldolase enzyme cleaves a 6-carbon fructose sugar into two 3-carbon products in a reverse
The ΔG°’ of this reaction is +23.9 kJ/mol. Though the reaction may seem too uphill to occur, it is of note that under physiological conditions, the ΔG of the reaction falls to close to or below zero. For example, the ΔG of this reaction under physiological conditions in erythrocytes is -0.23 kJ/mol.[10]
Structure
Aldolase B is a homotetrameric enzyme, composed of four subunits with molecular weights of 36 kDa with local 222 symmetry. Each subunit has a molecular weight of 36 kDa and contains an eight-stranded α/β barrel, which encloses lysine 229 (the Schiff-base forming amino acid that is key for catalysis).[11][12]
Isozyme specific regions
Though the majority of the overall structure of the aldolase enzyme is conserved amongst the three isozymes, four regions of the generic aldolase enzyme have been identified to be highly variable among isozymes. Such regions have been denoted isozyme-specific regions (ISR1-4). These regions are thought to give isozymes their specificities and structural differences. ISRs 1-3 are all found in exon 3 of the ALDOB gene. ISR 4 is the most variable of the four and is found at the c-terminal end of the protein.[5]
ISRs 1-3 are found predominantly in patches on the surface of the enzyme. These patches do not overlap with the active site, indicating that ISRs may change specific isozyme substrate specificity from a distance or cause the C-terminus interactions with the active site.[12] A recent theory suggests that ISRs may allow for different conformational dynamics in the aldolase enzyme that account for its specificity.[13]
Physiology
Aldolase B plays a key role in
Though the mechanism aldolase B regulation is unknown, increased ALDOB gene transcription in animal livers has been noticed with an increase in dietary carbohydrates and decrease in glucagon concentration.[15][16]
Interactive pathway map
Click on genes, proteins and metabolites below to link to respective articles.[§ 1]
- ^ The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534".
Pathology
Genetic mutations leading to defects in aldolase B result in a condition called hereditary fructose intolerance. Due to the lack of functional aldolase B, organisms with HFI cannot properly process F1P, which leads to an accumulation of F1P in bodily tissues. In addition to being toxic to cellular tissues, high levels of F1P traps phosphate in an unusable form that does not return to the general phosphate pool, resulting in depletion of both phosphate and ATP stores. The lack of readily available phosphate causes the cessation of glycogenolysis in the liver, which results in hypoglycemia.[17] This accumulation also inhibits gluconeogenesis, further reducing the amount of readily available glucose. The loss of ATP leads to a multitude of problems including inhibition of protein synthesis and hepatic and renal dysfunction. Patient prognosis, however, is good in cases of hereditary fructose intolerance. By avoiding foods containing fructose, sucrose, and sorbitol, patients can live symptom-free lives.[14]
HFI is recessively inherited autosomal disorder. Approximately 30 mutations that cause HFI have been identified, and these combined mutations result in a HFI frequency of 1 in every 20,000 births.[14][18] Mutant alleles are a result of a number different types of mutations including base pair substitutions and small deletions. The most common mutation is A149P, which is a guanine to cytosine transversion in exon 5, resulting in the replacement of alanine at position 149 with proline. This specific mutant allele is estimated to account for 53% of HFI alleles.[19] Other mutations resulting in HFI are less frequent and often correlated with ancestral origins.[20]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000136872 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028307 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ PMID 11679716.
- ^ "Entrez Gene: ALDOB aldolase B, fructose-bisphosphate".
- S2CID 10058239.
- PMID 3016456.
- S2CID 39102274.
- ^ a b Garrett RH, Grisham CM (2010). Biochemistry (4th ed.). Brooks/Cole.
- PMID 3479768.
- ^ PMID 12611890.
- PMID 17935305.
- ^ a b c Inborn Metabolic Diseases (Fourth Revised ed.). Springer Berlin Heidelberg. 2006.
- PMID 7979370.
- PMID 2984252.
- S2CID 207099820.
- S2CID 7134716.
- PMID 15733923.
- S2CID 35127545.
Further reading
- Cross NC, de Franchis R, Sebastio G, et al. (1990). "Molecular analysis of aldolase B genes in hereditary fructose intolerance". Lancet. 335 (8685): 306–9. S2CID 1522710.
- Cross NC, Stojanov LM, Cox TM (1990). "A new aldolase B variant, N334K, is a common cause of hereditary fructose intolerance in Yugoslavia". Nucleic Acids Res. 18 (7): 1925. PMID 2336380.
- Sakakibara M, Mukai T, Yatsuki H, Hori K (1985). "Human aldolase isozyme gene: the structure of multispecies aldolase B mRNAs". Nucleic Acids Res. 13 (14): 5055–69. PMID 2410860.
- Sakakibara M, Takahashi I, Takasaki Y, et al. (1989). "Construction and expression of human aldolase A and B expression plasmids in Escherichia coli host". Biochim. Biophys. Acta. 1007 (3): 334–42. PMID 2649152.
- Mukai T, Yatsuki H, Arai Y, et al. (1988). "Human aldolase B gene: characterization of the genomic aldolase B gene and analysis of sequences required for multiple polyadenylations". J. Biochem. 102 (5): 1043–51. PMID 2830249.
- Cross NC, Tolan DR, Cox TM (1988). "Catalytic deficiency of human aldolase B in hereditary fructose intolerance caused by a common missense mutation". Cell. 53 (6): 881–5. S2CID 31460581.
- Paolella G, Santamaria R, Izzo P, et al. (1984). "Isolation and nucleotide sequence of a full-length cDNA coding for aldolase B from human liver". Nucleic Acids Res. 12 (19): 7401–10. PMID 6548561.
- Rottmann WH, Tolan DR, Penhoet EE (1984). "Complete amino acid sequence for human aldolase B derived from cDNA and genomic clones". Proc. Natl. Acad. Sci. U.S.A. 81 (9): 2738–42. PMID 6585824.
- Besmond C, Dreyfus JC, Gregori C, et al. (1984). "Nucleotide sequence of a cDNA clone for human aldolase B". Biochem. Biophys. Res. Commun. 117 (2): 601–9. PMID 6689266.
- Ali M, Cox TM (1995). "Diverse mutations in the aldolase B gene that underlie the prevalence of hereditary fructose intolerance". Am. J. Hum. Genet. 56 (4): 1002–5. PMID 7717389.
- Ali M, Sebastio G, Cox TM (1994). "Identification of a novel mutation (Leu 256→Pro) in the human aldolase B gene associated with hereditary fructose intolerance". Hum. Mol. Genet. 3 (1): 203–4. PMID 8162030.
- Brooks CC, Tolan DR (1994). "A partially active mutant aldolase B from a patient with hereditary fructose intolerance". FASEB J. 8 (1): 107–13. S2CID 7577134.
- Kusakabe T, Motoki K, Hori K (1997). "Mode of interactions of human aldolase isozymes with cytoskeletons". Arch. Biochem. Biophys. 344 (1): 184–93. PMID 9244396.
- Lau J, Tolan DR (1999). "Screening for hereditary fructose intolerance mutations by reverse dot-blot". Mol. Cell. Probes. 13 (1): 35–40. PMID 10024431.
- Santamaria R, Esposito G, Vitagliano L, et al. (2001). "Functional and molecular modelling studies of two hereditary fructose intolerance-causing mutations at arginine 303 in human liver aldolase". Biochem. J. 350 Pt 3 (Pt 3): 823–8. PMID 10970798.
- Susan PP, Dunn WA (2001). "Starvation-induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone-mediated transport". J. Cell. Physiol. 187 (1): 48–58. S2CID 32109377.
External links
- Aldolase+B at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
- Human ALDOB genome location and ALDOB gene details page in the UCSC Genome Browser.