Angular resolution
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Angular resolution describes the ability of any
Definition of terms
Resolving power is the ability of an imaging device to separate (i.e., to see as distinct) points of an object that are located at a small
The Rayleigh criterion
The imaging system's resolution can be limited either by
The interplay between diffraction and aberration can be characterised by the
Considering diffraction through a circular aperture, this translates into:
where θ is the angular resolution (
The formal Rayleigh criterion is close to the
Using a small-angle approximation, the angular resolution may be converted into a spatial resolution, Δℓ, by multiplication of the angle (in radians) with the distance to the object. For a microscope, that distance is close to the focal length f of the objective. For this case, the Rayleigh criterion reads:
- .
This is the
A similar result holds for a small sensor imaging a subject at infinity: The angular resolution can be converted to a spatial resolution on the sensor by using f as the distance to the image sensor; this relates the spatial resolution of the image to the f-number, f/#:
- .
Since this is the radius of the Airy disk, the resolution is better estimated by the diameter,
Specific cases
Single telescope
Point-like sources separated by an
The angular resolution R of a telescope can usually be approximated by
where λ is the
This formula, for light with a wavelength of about 562 nm, is also called the Dawes' limit.
Telescope array
The highest angular resolutions for telescopes can be achieved by arrays of telescopes called astronomical interferometers: These instruments can achieve angular resolutions of 0.001 arcsecond at optical wavelengths, and much higher resolutions at x-ray wavelengths. In order to perform aperture synthesis imaging, a large number of telescopes are required laid out in a 2-dimensional arrangement with a dimensional precision better than a fraction (0.25x) of the required image resolution.
The angular resolution R of an interferometer array can usually be approximated by
where λ is the
For example, in order to form an image in yellow light with a wavelength of 580 nm, for a resolution of 1 milli-arcsecond, we need telescopes laid out in an array that is 120 m × 120 m with a dimensional precision better than 145 nm.
Microscope
The resolution R (here measured as a distance, not to be confused with the angular resolution of a previous subsection) depends on the angular aperture :[5]
- where .
Here NA is the numerical aperture, is half the included angle of the lens, which depends on the diameter of the lens and its focal length, is the refractive index of the medium between the lens and the specimen, and is the wavelength of light illuminating or emanating from (in the case of fluorescence microscopy) the sample.
It follows that the NAs of both the objective and the condenser should be as high as possible for maximum resolution. In the case that both NAs are the same, the equation may be reduced to:
The practical limit for is about 70°. In a dry objective or condenser, this gives a maximum NA of 0.95. In a high-resolution
which is near 200 nm.
Oil immersion objectives can have practical difficulties due to their shallow depth of field and extremely short working distance, which calls for the use of very thin (0.17 mm) cover slips, or, in an inverted microscope, thin glass-bottomed Petri dishes.
However, resolution below this theoretical limit can be achieved using super-resolution microscopy. These include optical near-fields (Near-field scanning optical microscope) or a diffraction technique called 4Pi STED microscopy. Objects as small as 30 nm have been resolved with both techniques.[6][7] In addition to this Photoactivated localization microscopy can resolve structures of that size, but is also able to give information in z-direction (3D).
List of telescopes and arrays by angular resolution
Name | Image | Angular resolution ( arc seconds ) |
Wavelength | Type | Site | Year |
---|---|---|---|---|---|---|
Global mm-VLBI Array (successor to the Coordinated Millimeter VLBI Array) | 0.000012 (12 μas) | radio (at 1.3 cm) | very long baseline interferometry array of different radio telescopes |
a range of locations on Earth and in space[8] | 2002 - | |
PIONIER |
0.001 (1 mas) | light (1-2 micrometre)[9] | largest optical array of 4 reflecting telescopes |
Paranal Observatory, Antofagasta Region, Chile | 2002/2010 - | |
Hubble Space Telescope | 0.04 | light (near 500 nm)[10] | space telescope | Earth orbit | 1990 - | |
James Webb Space Telescope | 0.1[11] | infrared (at 2000 nm)[12] | space telescope | Sun–Earth L2 | 2022 - |
See also
- Angular diameter
- Beam diameter
- Dawes' limit
- Diffraction-limited system
- Ground sample distance
- Image resolution
- Optical resolution
- Sparrow's resolution limit
- Visual acuity
Notes
- ^ In the case of laser beams, a Gaussian Optics analysis is more appropriate than the Rayleigh criterion, and may reveal a smaller diffraction-limited spot size than that indicated by the formula above.
References
- ^
ISBN 0-521-64222-1.
- ^ a b Lord Rayleigh, F.R.S. (1879). "Investigations in optics, with special reference to the spectroscope". .
- ^
Michalet, X. (2006). "Using photon statistics to boost microscopy resolution". PMID 16549771.
- ^ "Diffraction: Fraunhofer Diffraction at a Circular Aperture" (PDF). Melles Griot Optics Guide. Melles Griot. 2002. Archived from the original (PDF) on 2011-07-08. Retrieved 2011-07-04.
- ^ Davidson, M. W. "Resolution". Nikon’s MicroscopyU. Nikon. Retrieved 2017-02-01.
- ^
Pohl, D. W.; Denk, W.; Lanz, M. (1984). "Optical stethoscopy: Image recording with resolution λ/20". doi:10.1063/1.94865.
- ^ Dyba, M. "4Pi-STED-Microscopy..." Max Planck Society, Department of NanoBiophotonics. Retrieved 2017-02-01.
- ^ "Images at the Highest Angular Resolution in Astronomy". Max Planck Institute for Radio Astronomy. 2022-05-13. Retrieved 2022-09-26.
- arXiv:1701.01249.
- ^ "Hubble Space Telescope". NASA. 2007-04-09. Retrieved 2022-09-27.
- arXiv:1507.04779 [astro-ph.IM].
- ^ "FAQ Full General Public Webb Telescope/NASA". jwst.nasa.gov. 2002-09-10. Retrieved 2022-09-27.
External links
- "Concepts and Formulas in Microscopy: Resolution" by Michael W. Davidson, Nikon MicroscopyU (website).