Autophagy
Autophagy (or autophagocytosis; from the Ancient Greek αὐτόφαγος, autóphagos, meaning "self-devouring"[1] and κύτος, kýtos, meaning "hollow")[2] is the natural, conserved degradation of the cell that removes unnecessary or dysfunctional components through a lysosome-dependent regulated mechanism.[3] It allows the orderly degradation and recycling of cellular components.[4][5] Although initially characterized as a primordial degradation pathway induced to protect against starvation, it has become increasingly clear that autophagy also plays a major role in the homeostasis of non-starved cells.[6] Defects in autophagy have been linked to various human diseases, including neurodegeneration and cancer, and interest in modulating autophagy as a potential treatment for these diseases has grown rapidly.[6][7]
Four forms of autophagy have been identified:
In disease, autophagy has been seen as an adaptive response to stress, promoting survival of the cell; but in other cases, it appears to promote cell death and
The word "autophagy" was in existence and frequently used from the middle of the 19th century.[13] In its present usage, the term autophagy was coined by Belgian biochemist Christian de Duve in 1963 based on his discovery of the functions of lysosome.[3] The identification of autophagy-related genes in yeast in the 1990s allowed researchers to deduce the mechanisms of autophagy,[14][15][16][17][18] which eventually led to the award of the 2016 Nobel Prize in Physiology or Medicine to Japanese researcher Yoshinori Ohsumi.[19]
History
Autophagy was first observed by
In the 1990s several groups of scientists independently discovered autophagy-related genes using the budding yeast. Notably, Yoshinori Ohsumi and Michael Thumm examined starvation-induced non-selective autophagy;[15][16][17] in the meantime, Daniel J. Klionsky discovered the cytoplasm-to-vacuole targeting (CVT) pathway, which is a form of selective autophagy.[14][18] They soon found that they were in fact looking at essentially the same pathway, just from different angles.[26][27] Initially, the genes discovered by these and other yeast groups were given different names (APG, AUT, CVT, GSA, PAG, PAZ, and PDD). A unified nomenclature was advocated in 2003 by the yeast researchers to use ATG to denote autophagy genes.[28] The 2016 Nobel Prize in Physiology or Medicine was awarded to Yoshinori Ohsumi,[19] although some have pointed out that the award could have been more inclusive.[29]
The field of autophagy research experienced accelerated growth at the turn of the 21st century. Knowledge of ATG genes provided scientists more convenient tools to dissect functions of autophagy in human health and disease. In 1999, a landmark discovery connecting autophagy with cancer was published by Beth Levine's group.[30] To this date, relationship between cancer and autophagy continues to be a main theme of autophagy research. The roles of autophagy in neurodegeneration and immune defense also received considerable attention. In 2003, the first Gordon Research Conference on autophagy was held at Waterville.[31] In 2005, Daniel J Klionsky launched Autophagy, a scientific journal dedicated to this field. The first Keystone Symposia on autophagy was held in 2007 at Monterey.[32] In 2008, Carol A Mercer created a BHMT fusion protein (GST-BHMT), which showed starvation-induced site-specific fragmentation in cell lines. The degradation of betaine homocysteine methyltransferase (BHMT), a metabolic enzyme, could be used to assess autophagy flux in mammalian cells. Macro, micro, and Chaperone mediated autophagy are mediated by autophagy-related genes and their associated enzymes.[11][12][33][34][35] Macroautophagy is then divided into bulk and selective autophagy. In the selective autophagy is the autophagy of organelles; mitophagy,[36] lipophagy,[37] pexophagy,[38] chlorophagy,[39] ribophagy[40] and others.
Macroautophagy is the main pathway, used primarily to eradicate damaged cell
Microautophagy, on the other hand, involves the direct engulfment of cytoplasmic material into the lysosome.[45] This occurs by invagination, meaning the inward folding of the lysosomal membrane, or cellular protrusion.[42]
Lipophagy is the degradation of lipids by autophagy,
Targeted interplay between bacterial pathogens and host autophagy
Autophagy targets genus-specific proteins, so orthologous proteins which share sequence homology with each other are recognized as substrates by a particular autophagy targeting protein. There exists a complementarity of autophagy targeting proteins which potentially increase infection risk upon mutation. The lack of overlap among the targets of the 3 autophagy proteins and the large overlap in terms of the genera show that autophagy could target different sets of bacterial proteins from a same pathogen. On one hand, the redundancy in targeting a same genera is beneficial for robust pathogen recognition. But, on the other hand, the complementarity in the specific bacterial proteins could make the host more susceptible to chronic disorders and infections if the gene encoding one of the autophagy targeting proteins becomes mutated, and the autophagy system is overloaded or suffers other malfunctions. Moreover, autophagy targets virulence factors and virulence factors responsible for more general functions such as nutrient acquisition and motility are recognized by multiple autophagy targeting proteins. And the specialized virulence factors such as autolysins, and iron sequestering proteins are potentially recognized uniquely by a single autophagy targeting protein. The autophagy proteins CALCOCO2/NDP52 and MAP1LC3/LC3 may have evolved specifically to target pathogens or pathogenic proteins for autophagic degradation. While SQSTM1/p62 targets more generic bacterial proteins containing a target motif but not related to virulence.[51]
On the other hand, bacterial proteins from various pathogenic genera are also able to modulate autophagy. There are genus-specific patterns in the phases of autophagy that are potentially regulated by a given pathogen group. Some autophagy phases can only be modulated by particular pathogens, while some phases are modulated by multiple pathogen genera. Some of the interplay-related bacterial proteins have proteolytic and post-translational activity such as phosphorylation and ubiquitination and can interfere with the activity of autophagy proteins.[51]
Molecular biology
Autophagy is executed by autophagy-related (Atg) genes. Prior to 2003, ten or more names were used, but after this point a unified nomenclature was devised by fungal autophagy researchers.[52] Atg or ATG stands for autophagy related. It does not specify gene or a protein.[52]
The first autophagy genes were identified by genetic screens conducted in Saccharomyces cerevisiae.
In mammals,
Once active, VPS34 phosphorylates the lipid phosphatidylinositol to generate phosphatidylinositol 3-phosphate (PtdIns(3)P) on the surface of the phagophore. The generated PtdIns(3)P is used as a docking point for proteins harboring a PtdIns(3)P binding motif. WIPI2, a PtdIns(3)P binding protein of the WIPI (WD-repeat protein interacting with phosphoinositides) protein family, was recently shown to physically bind ATG16L1.[62] Atg16L1 is a member of an E3-like protein complex involved in one of two ubiquitin-like conjugation systems essential for autophagosome formation. The FIP200 cis-Golgi-derived membranes fuse with ATG16L1-positive endosomal membranes to form the prophagophore termed HyPAS (hybrid pre-autophagosomal structure).[63] ATG16L1 binding to WIPI2[64] mediates ATG16L1's activity. This leads to downstream conversion of prophagophore into ATG8-positive phagophore[63] via a ubiquitin-like conjugation system.
The first of the two
Sirtuin 1 (SIRT1) stimulates autophagy by preventing acetylation of proteins (via deacetylation) required for autophagy as demonstrated in cultured cells and embryonic and neonatal tissues.[74] This function provides a link between sirtuin expression and the cellular response to limited nutrients due to caloric restriction.[75]
Functions
Nutrient starvation
Autophagy has roles in various cellular functions. One particular example is in yeasts, where the nutrient starvation induces a high level of autophagy. This allows unneeded proteins to be degraded and the amino acids recycled for the synthesis of proteins that are essential for survival.[76][77][78] In higher eukaryotes, autophagy is induced in response to the nutrient depletion that occurs in animals at birth after severing off the trans-placental food supply, as well as that of nutrient starved cultured cells and tissues.[79][80] Mutant yeast cells that have a reduced autophagic capability rapidly perish in nutrition-deficient conditions.[81] Studies on the apg mutants suggest that autophagy via autophagic bodies is indispensable for protein degradation in the vacuoles under starvation conditions, and that at least 15 APG genes are involved in autophagy in yeast.[81] A gene known as ATG7 has been implicated in nutrient-mediated autophagy, as mice studies have shown that starvation-induced autophagy was impaired in atg7-deficient mice.[80]
Infection
Repair mechanism
Autophagy degrades damaged organelles, cell membranes and proteins, and insufficient autophagy is thought to be one of the main reasons for the accumulation of damaged cells and
Programmed cell death
One of the mechanisms of programmed cell death (PCD) is associated with the appearance of autophagosomes and depends on autophagy proteins. This form of cell death most likely corresponds to a process that has been morphologically defined as autophagic PCD. One question that constantly arises, however, is whether autophagic activity in dying cells is the cause of death or is actually an attempt to prevent it. Morphological and histochemical studies have not so far proved a causative relationship between the autophagic process and cell death. In fact, there have recently been strong arguments that autophagic activity in dying cells might actually be a survival mechanism.[85][86] Studies of the metamorphosis of insects have shown cells undergoing a form of PCD that appears distinct from other forms; these have been proposed as examples of autophagic cell death.[87] Recent pharmacological and biochemical studies have proposed that survival and lethal autophagy can be distinguished by the type and degree of regulatory signaling during stress particularly after viral infection.[88] Although promising, these findings have not been examined in non-viral systems.
Meiosis
Mammalian fetal oocytes face several challenges to survival throughout the stages of meiotic prophase I prior to primordial follicle assembly.[89] Each primordial follicle contains an immature primary oocyte. Before oocytes are enclosed into a primordial follicle, deficiencies of nutrients or growth factors might activate protective autophagy, but his can turn into death of the oocytes if starvation is prolonged.[89]
Exercise
Autophagy is essential for basal
Another study demonstrated that
Work at the Institute for Cell Biology, University of Bonn, showed that a certain type of autophagy, i.e.
Osteoarthritis
Because autophagy decreases with age and age is a major risk factor for
Cancer
Cancer often occurs when several different pathways that regulate cell differentiation are disturbed. Autophagy plays an important role in cancer – both in protecting against cancer as well as potentially contributing to the growth of cancer.[85][99] Autophagy can contribute to cancer by promoting survival of tumor cells that have been starved, or that degrade apoptotic mediators through autophagy: in such cases, use of inhibitors of the late stages of autophagy (such as chloroquine), on the cells that use autophagy to survive, increases the number of cancer cells killed by antineoplastic drugs.[100]
The role of autophagy in cancer is one that has been highly researched and reviewed. There is evidence that emphasizes the role of autophagy as both a tumor suppressor and a factor in tumor cell survival. Recent research has shown, however, that autophagy is more likely to be used as a tumor suppressor according to several models.[99]
Tumor suppressor
Several experiments have been done with mice and varying Beclin1, a protein that regulates autophagy. When the Beclin1 gene was altered to be heterozygous (Beclin 1+/-), the mice were found to be tumor-prone.[101] However, when Beclin1 was overexpressed, tumor development was inhibited.[30] Care should be exercised when interpreting phenotypes of beclin mutants and attributing the observations to a defect in autophagy, however: Beclin1 is generally required for phosphatidylinositol 3- phosphate production and as such it affects numerous lysosomal and endosomal functions, including endocytosis and endocytic degradation of activated growth factor receptors. In support of the possibility that Beclin1 affects cancer development through an autophagy-independent pathway is the fact that core autophagy factors which are not known to affect other cellular processes and are definitely not known to affect cell proliferation and cell death, such as Atg7 or Atg5, show a much different phenotype when the respective gene is knocked out, which does not include tumor formation. In addition, full knockout of Beclin1 is embryonic lethal whereas knockout of Atg7 or Atg5 is not.
Necrosis and chronic inflammation also has been shown to be limited through autophagy which helps protect against the formation of tumor cells.[102]
Colon cancer
Colon cancer incidence is associated with a high-fat diet, and such a diet is linked to elevated levels of bile acids in the colon, particularly deoxycholic acid.[103] Deoxycholic acid induces autophagy in non-cancer colon epithelial cells and this induction of autophagy contributes to cell survival when cells are stressed.[104] Also autophagy is a survival pathway that is constitutively present in apoptosis-resistant colon cancer cells.[104] The constitutive activation of autophagy in colon cancer cells, is thus a colon cancer cell survival strategy that needs to be overcome in colon cancer therapy.[104]
Mechanism of cell death
Cells that undergo an extreme amount of stress experience cell death either through apoptosis or necrosis. Prolonged autophagy activation leads to a high turnover rate of proteins and organelles. A high rate above the survival threshold may kill cancer cells with a high apoptotic threshold.[105][106] This technique can be utilized as a therapeutic cancer treatment.[85]
Tumor cell survival
Alternatively, autophagy has also been shown to play a large role in tumor cell survival. In cancerous cells, autophagy is used as a way to deal with stress on the cell.[107] Induction of autophagy by miRNA-4673, for example, is a pro-survival mechanism that improves the resistance of cancer cells to radiation.[108] Once these autophagy related genes were inhibited, cell death was potentiated.[109] The increase in metabolic energy is offset by autophagy functions. These metabolic stresses include hypoxia, nutrient deprivation, and an increase in proliferation. These stresses activate autophagy in order to recycle ATP and maintain survival of the cancerous cells.[105] Autophagy has been shown to enable continued growth of tumor cells by maintaining cellular energy production. By inhibiting autophagy genes in these tumors cells, regression of the tumor and extended survival of the organs affected by the tumors were found. Furthermore, inhibition of autophagy has also been shown to enhance the effectiveness of anticancer therapies.[105]
Therapeutic target
New developments in research have found that targeted autophagy may be a viable therapeutic solution in fighting cancer. As discussed above, autophagy plays both a role in tumor suppression and tumor cell survival. Thus, the qualities of autophagy can be used as a strategy for cancer prevention. The first strategy is to induce autophagy and enhance its tumor suppression attributes. The second strategy is to inhibit autophagy and thus induce apoptosis.[109]
The first strategy has been tested by looking at dose-response anti-tumor effects during autophagy-induced therapies. These therapies have shown that autophagy increases in a dose-dependent manner. This is directly related to the growth of cancer cells in a dose-dependent manner as well.[107][106] These data support the development of therapies that will encourage autophagy. Secondly, inhibiting the protein pathways directly known to induce autophagy may also serve as an anticancer therapy.[109][106]
The second strategy is based on the idea that autophagy is a protein degradation system used to maintain homeostasis and the findings that inhibition of autophagy often leads to apoptosis. Inhibition of autophagy is riskier as it may lead to cell survival instead of the desired cell death.[107]
Negative regulators of autophagy
Negative regulators of autophagy, such as
The interface between inflammation and autophagy
Regulators of autophagy control regulators of inflammation, and vice versa.[111] Cells of vertebrate organisms normally activate inflammation to enhance the capacity of the immune system to clear infections and to initiate the processes that restore tissue structure and function.[112] Therefore, it is critical to couple regulation of mechanisms for removal of cellular and bacterial debris to the principal factors that regulate inflammation: The degradation of cellular components by the lysosome during autophagy serves to recycle vital molecules and generate a pool of building blocks to help the cell respond to a changing microenvironment.[113] Proteins that control inflammation and autophagy form a network that is critical for tissue functions, which is dysregulated in cancer: In cancer cells, aberrantly expressed and mutant proteins increase the dependence of cell survival on the “rewired” network of proteolytic systems that protects malignant cells from apoptotic proteins and from recognition by the immune system.[114] This renders cancer cells vulnerable to intervention on regulators of autophagy.
Type 2 diabetes
Excessive activity of the crinophagy form of autophagy in the insulin-producing
See also
- Apoptosis – Programmed cell death in multicellular organisms
- Autophagy database
- Autophagin – Protease
- Mitophagy – autophagic process in which mitochondria are delivered to the vacuole and degraded
- Residual body – vesicles containing indigestible materials, part of lysosomal digestion
- Sub-lethal damage– Damaging changes to a biological cell
References
- ^ Liddell HG, Scott R, Jone HS. "αὐτό-φαγος". A Greek–English Lexicon. tufts.edu. Retrieved 6 September 2018.
- ^ Liddell HG, Scott R, Jone HS. "κύτος". A Greek–English Lexicon. tufts.edu. Retrieved 6 September 2018.
- ^ S2CID 6198427.
- PMID 22078875.
- PMID 26235572.
- ^ S2CID 209518157.
- PMID 28751651.
- ^ S2CID 212903191.
- PMID 23725295.
- S2CID 264073704.
- ^ PMID 21801009.
- ^ S2CID 26402002.
- PMID 28837378.
- ^ PMID 1400574.
- ^ PMID 1400575.
- ^ S2CID 26072787.
- ^ S2CID 46017791.
- ^ PMID 7593182.
- ^ a b "The Nobel Prize in Physiology or Medicine 2016". The Nobel Foundation. 3 October 2016. Retrieved 3 October 2016.
- PMID 13862833.
- PMID 13955261.
- PMID 6055998.
- PMID 4292315.
- PMID 6319122.
- ISBN 9781461465614.
- PMID 8663607.
- PMID 8901576.
- S2CID 39590247.
- PMID 27708326.
- ^ S2CID 4423132.
- ^ "Autophagy in Stress, Development & Disease". Gordon Research Conference. 2003.
- Keystone Symposia on Molecular and Cellular Biology. 2007. Archived from the originalon 2018-11-16. Retrieved 2016-10-04.
- ^ PMID 22187934.
- ^ PMID 12576635.
- ^ PMID 21179058.
- PMID 22944659.
- ^ PMID 22595754.
- PMID 22536249.
- S2CID 30079770.
- PMID 29230017.
- ^ PMID 21248839.
- ^ PMID 22465226.[permanent dead link]
- S2CID 17184033.
- ^ a b Homma KS (2011). "List of autophagy-related proteins and 3D structures". Autophagy Database. 290. Archived from the original on 2012-08-01. Retrieved 2012-10-08.
- ^ Castro-Obregon S (2010). "The Discovery of Lysosomes and Autophagy". Nature Education. 3 (9): 49.
- PMID 18644871.
- PMID 26778751.
- PMID 29309625.
- PMID 24258026.
- PMID 19339967.
- ^ PMID 30909843. Text was copied from this source, which is available under a Creative Commons Attribution 4.0 International License.
- ^ PMID 22889836.
- ^ S2CID 24083190.
- PMID 27723509.
- PMID 24343578.
- PMID 22074133.
- PMID 23685627.
- PMID 18843052.
- PMID 21311563.
- PMID 20921139.
- PMID 18443221.
- ^ T. Proikas-Cézanne, Z. Takacs, P. Donnes, and O. Kohlbacher, 'Wipi Proteins: Essential Ptdins3p Effectors at the Nascent Autophagosome', J Cell Sci, 128 (2015), 207-17
- ^ PMID 34741801.
- PMID 24954904.
- PMID 17986448.
- PMID 15169837.
- PMID 18768752.
- PMID 25483962.
- PMID 19781582.
- PMID 20089838.
- PMID 25533187.
- PMID 19322194.
- PMID 17021250.
- PMID 32397145.
- PMID 18296641.
- PMID 12455967.
- PMID 11099404.
- PMID 15068787.
- S2CID 4424974.
- ^ PMID 14699058.
- ^ S2CID 46017791.
- PMID 15884975.
- PMID 22246324.
- PMID 16874025.
- ^ S2CID 89307028.
- PMID 16247500.
- PMID 8430112.
- PMID 24606695.
- ^ a b Klinger FG, Rossi V, De Felici M. Multifaceted programmed cell death in the mammalian fetal ovary. Int J Dev Biol. 2015;59(1-3):51-4. doi: 10.1387/ijdb.150063fk. PMID: 26374525
- PMID 25121614.
- ^ PMID 22082869.
- ^ PMID 22258505.
- ^ PMID 22024752.
- ^ S2CID 8885338.
- PMID 23434281.
- PMID 20187128.
- PMID 22034068.
- PMID 25708836.
- ^ a b Furuya, N., Liang, X.H., and Levin, B. 2004. Autophagy and cancer. In Autophagy. D.J. Klionsky editor. Landes Bioscience. Georgetown, Texas, USA. 244-253.
- PMID 25023646.
- PMID 14638851.
- PMID 18394557.
- ^ Bernstein H, Bernstein C. Bile acids as carcinogens in the colon and at other sites in the gastrointestinal system. Exp Biol Med (Maywood). 2023 Jan;248(1):79-89. doi: 10.1177/15353702221131858. Epub 2022 Nov 19. PMID: 36408538; PMCID: PMC9989147
- ^ a b c Payne CM, Crowley-Skillicorn C, Holubec H, Dvorak K, Bernstein C, Moyer MP, Garewal H, Bernstein H. Deoxycholate, an endogenous cytotoxin/genotoxin, induces the autophagic stress-survival pathway: implications for colon carcinogenesis. J Toxicol. 2009;2009:785907. doi: 10.1155/2009/785907. Epub 2009 May 10. PMID: 20130808; PMCID: PMC2814131
- ^ PMID 21878654.
- ^ PMID 26225250.
- ^ PMID 11212227.
- PMID 30341280.
- ^ PMID 16969128.
- S2CID 3424489.
- PMID 27694913.
- S2CID 205214291.
- S2CID 208035547.
- PMID 31782148.
Further reading
- Liu Y, PMID 22242963.
- Starokadomskyy P, Dmytruk KV (July 2013). "A bird's-eye view of autophagy". Autophagy. 9 (7): 1121–6. PMID 23615436.
- Tavassoly I (February 2015). Dynamics of Cell Fate Decision Mediated by the Interplay of Autophagy and Apoptosis in Cancer Cells: Mathematical Modeling and Experimental Observations. Springer Theses. Springer. S2CID 89307028.
- "Function and mechanism of autophagy and its key points". Biomentors.net.
External links
- Autophagy, a journal produced by Landes Bioscience and edited by DJ Klionsky
- LongevityMeme entry describing PubMed article on the effects of autophagy and lifespan
- Autophagolysosome on Drugs.com
- HADb, a Human Autophagy dedicated Database
- Autophagy DB, an autophagy database that covers all eukaryotes
- Self-Destructive Behavior in Cells May Hold Key to a Longer Life
- Exercise as Housecleaning for the Body
- The AIM center