C1orf27
Uncharacterized protein Chromosome 1 Open Reading Frame 27 is a protein in humans, encoded by the C1orf27 gene. It is accession number NM_017847.[1] This is a membrane protein that is 3926 base pairs long with the most extensive string of amino acids being 454aa long. C1orf27 exhibits cytoplasmic expression in epidermal tissues.[2] Predicted associated biological processes of the gene include cell fate specification and developmental properties.[citation needed]
Gene
Locus
This gene is located on
mRNA
Alternative splicing
There appear to be four isoforms due to splicing.[4] Two of those are truncated on the 3' end of the protein from 266aa and 396aa. Additional location of alternative splice sites are from 79aa to 102aa and 246aa to 260aa.
Protein
General properties
The primary encoded protein of C1orf27 consists of 454
Aliases
As with many other genes, there are some common
Composition
Computational analysis revealed the most abundant amino acid to be leucine at 10.1% of the total protein.[5] The second most abundant was serine which contributes to 8.6% of the total protein. Glutamic acid was third most abundant and contributes to 7.7% of the protein. This analysis also revealed that the protein appears to be deficient in tryptophan as it only contributes to 1.1% of the protein.[5] Based on the distribution of other amino acid types, there were five high scoring hydrophobic segments. There were also two transmembrane domains located at 82-98aa and 432-449aa.
Post-translational modifications
Interactor | Number of Predicted Sites | Function |
---|---|---|
N-myristoylation | 8 | Key components of signaling pathways, and typically promotes membrane binding essential for protein localization and/ or biological function[6] |
N-glycosylation | 4 | Increase protein stability by decreasing protein dynamics.[7] |
Protein Kinase C Phosphorylation | 7 | Enzymatic activity regulation.[8] |
Casein Kinase II Phosphorylation | 7 | Epidermal growth factor role.[9] |
Tyrosine Kinase Phosphorylation | 2 | Alterations to the structural conformation.[10] |
cAMP and cGMP Dependent Phosphorylation | 2 | Coordination of the active site conformation and enzymatic activity. |
C1orf27 is predicted to undergo multiple post translational modifications such as glycosylation, myristoylation, and phosphorylation.[11]
Interactions
There were eight interactions identified by Mentha., the function of which is unknown. The last two were THID2 and Q81kP6 which are both in bacillus anthracis.
Subcellular localization
The c1orf27 protein is likely cytoplasmic.[13] This was found with 55.5 reliability. The K-NN prediction was k=9/23 and the protein was found to be 55.6% cytoplasmic, 11.1% mitochondrial, 11.1% vacuolar, 11.1% cytoskeletal, and 11.1% golgi.
Structure
Alpha helices predicted in the c1orf27 protein are colored blue in the above picture. Beta sheets are pictured by the red arrows. Random coils are the purple strands between structures.
Expression
Overall, expression of c1orf27 seems to be ubiquitous. in mice.
Homology
Paralogs
There were no
Orthologs
There were
Molecular Evolution
The m value, or number of corrected amino acid changes per 100 residues, for the C1orf27 gene was graphed against the species divergence in millions of years. When compared to divergence graphs of fibrinogen and cytochrome C, it was determined that this gene closely resembles the evolutionary pattern observed in fibrinogen, suggesting a more rapid rate of evolution. M values for C1orf27 were calculated using the percentage of identity, when compared to humans, observed in the mRNA sequences of the orthologs using the formula derived from the Molecular Clock Hypothesis.
References
- ^ a b "Homo sapiens odr-4 GPCR localization factor homolog (ODR4), transcript - Nucleotide - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-02-05.
- ^ "Tissue expression of C1orf27 - Summary - The Human Protein Atlas". www.proteinatlas.org. Retrieved 2018-02-05.
- ^ a b "ODR4 odr-4 GPCR localization factor homolog [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-02-19.
- ^ a b c "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2018-02-25.
- ^ a b EMBL-EBI. "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 2018-04-22.
- PMID 19898886.
- ^ "Proteomics/Post-translational Modification/Glycosylation - Wikibooks, open books for an open world". en.wikibooks.org. Retrieved 2018-05-06.
- PMID 18295358.
- PMID 12446764.
- PMID 25161153.
- ^ "ExPASy - PROSITE". prosite.expasy.org. Retrieved 2018-04-22.
- ^ "mentha: the interactome browser". mentha.uniroma2.it. Retrieved 2018-04-22.
- ^ "PSORT II Prediction". psort.hgc.jp. Retrieved 2018-04-22.
- ^ Kelley, Lawrence. "PHYRE2 Protein Fold Recognition Server". www.sbg.bio.ic.ac.uk. Retrieved 2018-05-06.
- ^ "GDS1402 / NM_017847.1_PROBE1". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.
- ^ a b Group, Schuler. "EST Profile - Hs.371210". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.