Cauliflower mosaic virus

Cauliflower mosaic virus
Virus classification
(unranked):	Virus
Realm:	Riboviria
Kingdom:	Pararnavirae
Phylum:	Artverviricota
Class:	Revtraviricetes
Order:	Ortervirales
Family:	Caulimoviridae
Genus:	Caulimovirus
Species:	Cauliflower mosaic virus

Cauliflower mosaic virus (CaMV) is a member of the genus

Definition

The cauliflower mosaic virus (CaMV) is a member of the family

Blubervirales (both orders belong to the same class, the Revtraviricetes

).

CaMV infects mostly plants of the family Brassicaceae (such as cauliflower and turnip) but some CaMV strains (D4 and W260) are also able to infect Solanaceae species of the genera Datura and Nicotiana. CaMV induces a variety of systemic symptoms such as mosaic, necrotic lesions on leaf surfaces, stunted growth, and deformation of the overall plant structure. The symptoms exhibited vary depending on the viral strain, host ecotype, and environmental conditions.^[3]

CaMV is transmitted in a non-circulatory manner by aphid species such as Myzus persicae.^[4] Once introduced within a plant host cell, virions migrate to the nuclear envelope of the plant cell.

Structure

The CaMV particle is an icosahedron with a diameter of 52 nm built from 420 capsid protein (CP) subunits arranged with a triangulation T = 7, which surrounds a solvent-filled central cavity.^[5][6]

CaMV contains a circular double-stranded DNA molecule of about 8.0 kilobases, interrupted by nicks that result from the actions of RNAse H during reverse transcription. These nicks come from the Met-tRNA, and two RNA primers used in reverse transcription. After

Terminally redundant

Genome

The

The promoter was named CaMV 35S promoter ("35S promoter") because the coefficient of sedimentation of the viral transcript, whose expression is naturally driven by this promoter, is 35S. It is one of the most widely used, general-purpose constitutive promoters. It was discovered at the beginning of the 1980s, by Chua and collaborators at The Rockefeller University.

The 35S RNA is particularly complex, containing a highly structured 600 nucleotide long leader sequence with six to eight short open reading frames (ORFs).^[8][9][10]

This leader is followed by seven tightly arranged, longer ORFs that encode all the viral proteins. The mechanism of expression of these proteins is unique, in that the ORF VI protein (encoded by the 19S RNA) controls translation reinitiation of major open reading frames on the polycistronic 35S RNA, a process that normally only happens on bacterial mRNAs. TAV function depends on its association with

• ORF I – P1: movement protein (P03545)
• ORF II – P2: aphid/insect transmission factor (P03548)
• ORF III – P3: virion-associated protein (VAP, P03551): structural protein, DNA-binding capabilities
• ORF IV – P4: capsid protein (CP, P03542)
• ORF V – P5: pro-pol (P03554): protease, bifunctional reverse transcriptase and RNaseH
• ORF VI – P6: transactivator/viroplasmin (P03559): inclusion body Formation/trafficking; possibly other functions (see text)
• ORF VII/VIII – unknown (appears note to be required for infection, Q83163, Q83164)
  • Contains a
    tRNA
    -Met binding site

In addition to its functions regarding translational activation and formation of inclusion bodies, P6 has been shown to interact with a number of other CaMV proteins, such as P2 and P3, suggesting that it may also contribute in some degree to viral assembly and aphid-mediated transmission. In addition, P6 has been shown to bind to P7; investigating interactions between the two may help to elucidate the as yet unknown function of P7.^[12]

Another function of P6 involves modification of host NON-EXPRESSOR OF PATHOGENESIS RELATED 1 (NPR1) during the course of infection. NPR1 is an important regulator of salicylic acid (SA) and jasmonic acid (JA)-dependent signaling, and is most closely associated with crosstalk between the two. Modification of NPR1 serves to inhibit plant cells’ defensive responses by preventing SA-dependent signaling; modified NPR1 can properly traffic to the nucleus and bind the PR-1 promoter, but is unable to initiate transcription. Because active NPR1 is required for accumulation of SA, this leads to a further depletion of SA. Whereas regulation of SA-dependent signaling by P6-modified NPR1 is localized to the nucleus, regulation of JA-dependent signaling is cytoplasmic in nature and involves the COI1 pathway. In contrast to that of SA, JA-dependent signaling is increased in the presence of modified NPR1.^[13]

Replication

CaMV replicates by reverse transcription:

1. Viral particles enter a plant cell and are unencapsidated. At this stage the viral DNA consists of three fragments, one on the – strand (α) and two on the + strand (β and γ) which are imperfectly assembled into a circular genome with three gaps or discontinuities (D1, D2, and D3).
2. The viral DNA enters the
   histones
   , forming a minichromosome (not shown).
3. The host
   DNA-dependent RNA polymerase
   transcribes from the 35S promoter all the way around the viral genome, surpassing the 35S promoter. (This creates two copies of the 35S promoter in the resulting RNA.) Transcription also initiates at the 19S promoter (not shown).
4. The viral RNAs pass into the host cytoplasm where they are transcribed.
5. The 3′ end of a tRNA^fMet anneals to a site corresponding to discontinuity 1 (D1) near the 5′ end of the 35S RNA.
6. The tRNA
   fMet
   primes synthesis, by the viral reverse transcriptase (encoded by ORF V), of a new α strand.
7. RNase H
   removes the RNA from the DNA–RNA duplex, leaving behind the DNA.
8. This new DNA binds the 35S promoter at the 3′ end of the RNA template and synthesis of the α strand of DNA continues and RNase H continues to degrade RNA complexed to DNA.
9. Synthesis of the α strand completes. RNase H activity exposes purine-rich regions at the position of discontinuity 3 (D3), which primes the synthesis of the γ DNA strand.
10. RNase H activity exposes purine-rich regions at the position of discontinuity 2 (D2), which primes the synthesis of the β DNA strand. When the new γ strand of DNA reaches the 5′ end of the new α strand it switches to the 5′ end of the new α strand, recreating discontinuity 1 (D1). When the new γ strand of DNA reaches the 5′ end of the new β strand, it displaces the primer and some of the newly synthesized β strand, resulting in the recreation of discontinuity 2 (D2). When the new β strand of DNA reaches the 5′ end of the new γ strand, it displaces the primer and some of the newly synthesized γ strand, resulting in the recreation of discontinuity 3 (D3).

At this point the new viral genome can either be packaged into capsids and released from the cell or they can be transported by movement proteins into an adjacent, uninfected cell.^[14]

The cauliflower mosaic virus promoter (CaMV 35S) is used in most transgenic crops to activate foreign genes which have been artificially inserted into the host plant. It is inserted into transgenic plants in a form which is different from that found when it is present in its natural Brassica plant hosts. This enables it to operate in a wide range of host-organism environments which would otherwise not be possible.

CaMV contains about 8 kb double-strand DNA genome and produces spherical particles. CaMV infections are systemic, and even its DNA is infectious when inoculated on abraded plant surfaces. The CaMV genome has 8 tightly packed genes, of which only two small genes, genes II and VII, are nonessential; as a result, only these two genes can be replaced/deleted without a loss of infectivity. In addition, modified CaMV genomes exceeding the natural genome size (8024 bp) by even a few hundred bp are not packaged into virions. These two factors seriously limit the size of DNA insert clonable in CaMV. The bacterial dihydrofolate reductase

Molecular mechanisms of vector-mediated CaMV transmission

The virus is acquired from an infected host during feeding by the aphid vector. To occur, a transmissible complex is composed of virions and protein P2 located in the vector's stylets. The P2 N-terminal domain recognizes a protein receptor located at the tip of the stylet and the P2 C-terminal domain binds to the P3-decorated virions.^[15]

The mode of acquisition by the vector is controlled by the tissue and intracellular-specific localization of P2. This protein is only found in epidermis and parenchyma cells. Moreover, in these cells, P2 is localized in single viral electron-lucent inclusion bodies (ELIB).^[16] In host cells, viral protein P2 and P3 are first produced in numerous viral factories (electron-dense inclusion bodies), and are later exported and co-localize with microtubules, before concentrating in ELIB. CaMV specifically uses the microtubules to form the transmissible body and thus enable vector transmission.^[17] The complete molecular characterization and study of this virus was not carried further.

Evasion of plant defenses

Cauliflower mosaic virus possesses a number of mechanisms that allow it to counteract host plant cell defenses. While the pregenomic 35S RNA is responsible for genome replication by reverse transcriptase, it also contains a non-coding 600 base pair leader sequence that serves as an important mRNA for the production of factors involved in viral counter-defense. A number of hosts of CaMV possess small RNA-based viral silencing mechanisms that serve to limit viral infection. The products of the aforementioned 600-bp sequence are viral small RNAs (vsRNA) of 21, 22, and 24 nucleotides in length that serve as decoys, binding and inactivating effectors of host silencing machinery, such as Argonaute 1 (