Complement receptor 2
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| Location (UCSC) | Chr 1: 207.45 – 207.49 Mb | Chr 1: 194.82 – 194.86 Mb | |||||||
| PubMed search | [3] | [4] | |||||||
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Complement receptor type 2 (CR2), also known as complement C3d receptor, Epstein-Barr virus receptor, and CD21 (cluster of differentiation 21), is a protein that in humans is encoded by the CR2 gene.
CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.[5] B cells express CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. [6]
Interactions
Complement receptor 2
Isoforms
The canonical Cr2/CD21 gene of subprimate mammals produces two types of complement receptor (CR1, ca. 200 kDa; CR2, ca. 145 kDa) via alternative mRNA splicing. The murine Cr2 gene contains 25 exons; a common first exon is spliced to exon 2 and to exon 9 in transcripts encoding CR1 and CR2, respectively. A transcript with an open reading frame of 4,224 nucleotides encodes the long isoform, CR1; this is predicted to be a protein of 1,408 amino acids that includes 21 short consensus repeats (SCR) of ca. 60 amino acids each, plus transmembrane and cytoplasmic regions. Isoform CR2 (1,032 amino acids) is encoded by a shorter transcript (3,096 coding nucleotides) that lacks exons 2-8 encoding SCR1-6. CR1 and CR2 on murine B cells form complexes with a co-accessory activation complex containing CD19, CD81, and the fragilis/Ifitm (murine equivalents of LEU13) proteins.[11]
The CR2 gene of primates produces only the smaller isoform, CR2; primate complement receptor 1, which recapitulates many of the structural domains and presumed functions of Cr2-derived CR1 in subprimates, is encoded by a distinct CR1 gene (apparently derived from the gene Crry of subprimates).
Isoforms CR1 and CR2 derived from the non-primate Cr2 locus possess the same C-terminal sequence, such that association with and activation through CD19 should be equivalent. CR1 can bind to C4b and C3b complexes, whereas CR2 (murine and human) binds to C3dg-bound complexes. CR1, a surface protein produced primarily by
Immunohistochemistry
Although CR2 is present on all mature B-cells and follicular dendritic cells (FDCs), this becomes readily apparent only when immunohistochemistry is performed on frozen sections. In more conventional paraffin-embedded tissue samples, only the FDCs retain the staining pattern. As a result, CR2, more commonly called CD21 in the context of immunohistochemistry, can be used to demonstrate the FDC meshwork in lymphoid tissue.
This feature can be useful in examining tissue where the normal
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000117322 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026616 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ ISBN 0-7817-2120-2.
- ^ a b "Entrez Gene: CR2 complement component (3d/Epstein Barr virus) receptor 2".
- S2CID 23655762.
- PMID 9804823.
- ISBN 0-7216-0008-5
- S2CID 20793968.
- PMID 18713965.
- PMID 23733878.
- PMID 31269981.)
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- This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ - ISBN 978-1-84110-100-2.
Further reading
- Cooper NR, Bradt BM, Rhim JS, Nemerow GR (1990). "CR2 complement receptor". J. Invest. Dermatol. 94 (6 Suppl): s112–s117. PMID 2161885.
- Gauffre A, Viron A, Barel M, et al. (1992). "Nuclear localization of the Epstein-Barr virus/C3d receptor (CR2) in the human Burkitt B lymphoma cell, Raji". Mol. Immunol. 29 (9): 1113–20. PMID 1323059.
- Levy E, Ambrus J, Kahl L, et al. (1992). "T lymphocyte expression of complement receptor 2 (CR2/CD21): a role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE)". Clin. Exp. Immunol. 90 (2): 235–44. PMID 1424280.
- Matsumoto AK, Kopicky-Burd J, Carter RH, et al. (1991). "Intersection of the complement and immune systems: a signal transduction complex of the B lymphocyte-containing complement receptor type 2 and CD19". J. Exp. Med. 173 (1): 55–64. PMID 1702139.
- Tuveson DA, Ahearn JM, Matsumoto AK, Fearon DT (1991). "Molecular interactions of complement receptors on B lymphocytes: a CR1/CR2 complex distinct from the CR2/CD19 complex". J. Exp. Med. 173 (5): 1083–9. PMID 1708808.
- Kalli KR, Ahearn JM, Fearon DT (1991). "Interaction of iC3b with recombinant isotypic and chimeric forms of CR2". J. Immunol. 147 (2): 590–4. S2CID 24059423.
- Barel M, Gauffre A, Lyamani F, et al. (1991). "Intracellular interaction of EBV/C3d receptor (CR2) with p68, a calcium-binding protein present in normal but not in transformed B lymphocytes". J. Immunol. 147 (4): 1286–91. S2CID 22824326.
- Delcayre AX, Salas F, Mathur S, et al. (1991). "Epstein Barr virus/complement C3d receptor is an interferon alpha receptor". EMBO J. 10 (4): 919–26. PMID 1849076.
- Kurtz CB, Paul MS, Aegerter M, et al. (1989). "Murine complement receptor gene family. II. Identification and characterization of the murine homolog (Cr2) to human CR2 and its molecular linkage to Crry". J. Immunol. 143 (6): 2058–67. S2CID 20445833.
- Fujisaku A, Harley JB, Frank MB, et al. (1989). "Genomic organization and polymorphisms of the human C3d/Epstein-Barr virus receptor". J. Biol. Chem. 264 (4): 2118–25. PMID 2563370.
- Moore MD, Cooper NR, Tack BF, Nemerow GR (1988). "Molecular cloning of the cDNA encoding the Epstein-Barr virus/C3d receptor (complement receptor type 2) of human B lymphocytes". Proc. Natl. Acad. Sci. U.S.A. 84 (24): 9194–8. PMID 2827171.
- Weis JJ, Toothaker LE, Smith JA, et al. (1988). "Structure of the human B lymphocyte receptor for C3d and the Epstein-Barr virus and relatedness to other members of the family of C3/C4 binding proteins". J. Exp. Med. 167 (3): 1047–66. PMID 2832506.
- Weis JJ, Fearon DT, Klickstein LB, et al. (1986). "Identification of a partial cDNA clone for the C3d/Epstein-Barr virus receptor of human B lymphocytes: homology with the receptor for fragments C3b and C4b of the third and fourth components of complement". Proc. Natl. Acad. Sci. U.S.A. 83 (15): 5639–43. PMID 3016712.
- Weis JH, Morton CC, Bruns GA, et al. (1987). "A complement receptor locus: genes encoding C3b/C4b receptor and C3d/Epstein-Barr virus receptor map to 1q32". J. Immunol. 138 (1): 312–5. S2CID 28146101.
- Aubry JP, Pochon S, Gauchat JF, et al. (1994). "CD23 interacts with a new functional extracytoplasmic domain involving N-linked oligosaccharides on CD21". J. Immunol. 152 (12): 5806–13. S2CID 42434103.
- Matsumoto AK, Martin DR, Carter RH, et al. (1993). "Functional dissection of the CD21/CD19/TAPA-1/Leu-13 complex of B lymphocytes". J. Exp. Med. 178 (4): 1407–17. PMID 7690834.
- Barel M, Balbo M, Gauffre A, Frade R (1995). "Binding sites of the Epstein-Barr virus and C3d receptor (CR2, CD21) for its three intracellular ligands, the p53 anti-oncoprotein, the p68 calcium binding protein and the nuclear p120 ribonucleoprotein". Mol. Immunol. 32 (6): 389–97. PMID 7753047.
External links
- Complement+Receptors+2 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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