Cooperative binding
Cooperative binding occurs in molecular binding systems containing more than one type, or species, of molecule and in which one of the partners is not mono-valent and can bind more than one molecule of the other species. In general, molecular binding is an interaction between molecules that results in a stable physical association between those molecules.
Cooperative binding occurs in a molecular binding system where two or more ligand molecules can bind to a receptor molecule. Binding can be considered "cooperative" if the actual binding of the first molecule of the ligand to the receptor changes the binding affinity of the second ligand molecule. The binding of ligand molecules to the different sites on the receptor molecule do not constitute mutually independent events. Cooperativity can be positive or negative, meaning that it becomes more or less likely that successive ligand molecules will bind to the receptor molecule.
Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.
History and mathematical formalisms
Christian Bohr and the concept of cooperative binding
In 1904, Christian Bohr studied hemoglobin binding to oxygen under different conditions.[1][2] When plotting hemoglobin saturation with oxygen as a function of the partial pressure of oxygen, he obtained a sigmoidal (or "S-shaped") curve. This indicates that the more oxygen is bound to hemoglobin, the easier it is for more oxygen to bind - until all binding sites are saturated. In addition, Bohr noticed that increasing CO2 pressure shifted this curve to the right - i.e. higher concentrations of CO2 make it more difficult for hemoglobin to bind oxygen.[2] This latter phenomenon, together with the observation that hemoglobin's affinity for oxygen increases with increasing pH, is known as the Bohr effect.
A receptor molecule is said to exhibit cooperative binding if its binding to ligand scales non-linearly with ligand concentration. Cooperativity can be positive (if binding of a ligand molecule increases the receptor's apparent affinity, and hence increases the chance of another ligand molecule binding) or negative (if binding of a ligand molecule decreases affinity and hence makes binding of other ligand molecules less likely). The "fractional occupancy" of a receptor with a given ligand is defined as the quantity of ligand-bound binding sites divided by the total quantity of ligand binding sites:
If , then the protein is completely unbound, and if , it is completely saturated. If the plot of at equilibrium as a function of ligand concentration is sigmoidal in shape, as observed by Bohr for hemoglobin, this indicates positive cooperativity. If it is not, no statement can be made about cooperativity from looking at this plot alone.
The concept of cooperative binding only applies to molecules or complexes with more than one ligand binding sites. If several ligand binding sites exist, but ligand binding to any one site does not affect the others, the receptor is said to be non-cooperative. Cooperativity can be homotropic, if a ligand influences the binding of ligands of the same kind, or heterotropic, if it influences binding of other kinds of ligands. In the case of hemoglobin, Bohr observed homotropic positive cooperativity (binding of oxygen facilitates binding of more oxygen) and heterotropic negative cooperativity (binding of CO2 reduces hemoglobin's facility to bind oxygen.)
Throughout the 20th century, various frameworks have been developed to describe the binding of a ligand to a protein with more than one binding site and the cooperative effects observed in this context.[3]
The Hill equation
The first description of cooperative binding to a multi-site protein was developed by
where is the "Hill coefficient", denotes ligand concentration, denotes an apparent association constant (used in the original form of the equation), is an empirical dissociation constant, and a microscopic dissociation constant (used in modern forms of the equation, and equivalent to an ). If , the system exhibits negative cooperativity, whereas cooperativity is positive if . The total number of ligand binding sites is an upper bound for . The Hill equation can be linearized as:
The "Hill plot" is obtained by plotting versus . In the case of the Hill equation, it is a line with slope and intercept . This means that cooperativity is assumed to be fixed, i.e. it does not change with saturation. It also means that binding sites always exhibit the same affinity, and cooperativity does not arise from an affinity increasing with ligand concentration.
The Adair equation
G.S. Adair found that the Hill plot for hemoglobin was not a straight line, and hypothesized that binding affinity was not a fixed term, but dependent on ligand saturation.[5] Having demonstrated that hemoglobin contained four hemes (and therefore binding sites for oxygen), he worked from the assumption that fully saturated hemoglobin is formed in stages, with intermediate forms with one, two, or three bound oxygen molecules. The formation of each intermediate stage from unbound hemoglobin can be described using an apparent macroscopic association constant . The resulting fractional occupancy can be expressed as:
Or, for any protein with n ligand binding sites:
where n denotes the number of binding sites and each is a combined association constant, describing the binding of i ligand molecules. By combining the Adair treatment with the Hill plot, one arrives at the modern experimental definition of cooperativity (Hill, 1985, Abeliovich, 2005). The resultant Hill coefficient, or more correctly the slope of the Hill plot as calculated from the Adair Equation, can be shown to be the ratio between the variance of the binding number to the variance of the binding number in an equivalent system of non-interacting binding sites.[6] Thus, the Hill coefficient defines cooperativity as a statistical dependence of one binding site on the state of other site(s).
The Klotz equation
Working on calcium binding proteins, Irving Klotz deconvoluted Adair's association constants by considering stepwise formation of the intermediate stages, and tried to express the cooperative binding in terms of elementary processes governed by mass action law.[7][8] In his framework, is the association constant governing binding of the first ligand molecule, the association constant governing binding of the second ligand molecule (once the first is already bound) etc. For , this gives:
It is worth noting that the constants , and so forth do not relate to individual binding sites. They describe how many binding sites are occupied, rather than which ones. This form has the advantage that cooperativity is easily recognised when considering the association constants. If all ligand binding sites are identical with a microscopic association constant , one would expect (that is ) in the absence of cooperativity. We have positive cooperativity if lies above these expected values for .
The Klotz equation (which is sometimes also called the Adair-Klotz equation) is still often used in the experimental literature to describe measurements of ligand binding in terms of sequential apparent binding constants.[9]
Pauling equation
By the middle of the 20th century, there was an increased interest in models that would not only describe binding curves phenomenologically, but offer an underlying biochemical mechanism.
The KNF model
Based on results showing that the structure of cooperative proteins changed upon binding to their ligand,
Where is the constant of association for X, is the ratio of B and A states in the absence of ligand ("transition"), and are the relative stabilities of pairs of neighbouring subunits relative to a pair where both subunits are in the A state (Note that the KNF paper actually presents , the number of occupied sites, which is here 4 times ).
The MWC model
The
The allosteric isomerisation constant L describes the equilibrium between both states when no ligand molecule is bound: . If L is very large, most of the protein exists in the T state in the absence of ligand. If L is small (close to one), the R state is nearly as populated as the T state. The ratio of dissociation constants for the ligand from the T and R states is described by the constant c: . If , both R and T states have the same affinity for the ligand and the ligand does not affect isomerisation. The value of c also indicates how much the equilibrium between T and R states changes upon ligand binding: the smaller c, the more the equilibrium shifts towards the R state after one binding. With , fractional occupancy is described as:
The sigmoid Hill plot of allosteric proteins can then be analysed as a progressive transition from the T state (low affinity) to the R state (high affinity) as the saturation increases. The slope of the Hill plot also depends on saturation, with a maximum value at the inflexion point. The intercepts between the two asymptotes and the y-axis allow to determine the affinities of both states for the ligand.
In proteins, conformational change is often associated with activity, or activity towards specific targets. Such activity is often what is physiologically relevant or what is experimentally measured. The degree of conformational change is described by the state function , which denotes the fraction of protein present in the state. As the energy diagram illustrates, increases as more ligand molecules bind. The expression for is:
A crucial aspect of the MWC model is that the curves for and do not coincide,[14] i.e. fractional saturation is not a direct indicator of conformational state (and hence, of activity). Moreover, the extents of the cooperativity of binding and the cooperativity of activation can be very different: an extreme case is provide by the bacteria flagella motor with a Hill coefficient of 1.7 for the binding and 10.3 for the activation.[15][16] The supra-linearity of the response is sometimes called ultrasensitivity.
If an allosteric protein binds to a target that also has a higher affinity for the R state, then target binding further stabilizes the R state, hence increasing ligand affinity. If, on the other hand, a target preferentially binds to the T state, then target binding will have a negative effect on ligand affinity. Such targets are called allosteric modulators.
Since its inception, the MWC framework has been extended and generalized. Variations have been proposed, for example to cater for proteins with more than two states,[17] proteins that bind to several types of ligands [18][19] or several types of allosteric modulators [19] and proteins with non-identical subunits or ligand-binding sites.[20]
Examples
The list of molecular assemblies that exhibit cooperative binding of ligands is very large, but some examples are particularly notable for their historical interest, their unusual properties, or their physiological importance.
As described in the historical section, the most famous example of cooperative binding is hemoglobin. Its quaternary structure, solved by Max Perutz using X-ray diffraction,[21] exhibits a pseudo-symmetrical tetrahedron carrying four binding sites (hemes) for oxygen. Many other molecular assemblies exhibiting cooperative binding have been studied in great detail.
Multimeric enzymes
The activity of many enzymes is regulated by allosteric effectors. Some of these enzymes are multimeric and carry several binding sites for the regulators.
Threonine deaminase was one of the first enzymes suggested to behave like hemoglobin[22] and shown to bind ligands cooperatively.[23] It was later shown to be a tetrameric protein.[24]
Another enzyme that has been suggested early to bind ligands cooperatively is
Ion channels
Most ion channels are formed of several identical or pseudo-identical monomers or domains, arranged symmetrically in biological membranes. Several classes of such channels whose opening is regulated by ligands exhibit cooperative binding of these ligands.
It was suggested as early as 1967
Multi-site molecules
Although most proteins showing cooperative binding are multimeric complexes of homologous subunits, some proteins carry several binding sites for the same ligand on the same polypeptide. One such example is calmodulin. One molecule of calmodulin binds four calcium ions cooperatively.[33] Its structure presents four EF-hand domains,[34] each one binding one calcium ion. The molecule does not display a square or tetrahedron structure, but is formed of two lobes, each carrying two EF-hand domains.
Transcription factors
Cooperative binding of proteins onto nucleic acids has also been shown. A classical example is the binding of the
Conversely, examples of negative cooperativity for the binding of transcription factors were also documented, as for the homodimeric repressor of the Pseudomonas putida cytochrome P450cam hydroxylase operon[39] (n=0.56).
Conformational spread and binding cooperativity
Early on, it has been argued that some proteins, especially those consisting of many subunits, could be regulated by a generalized MWC mechanism, in which the transition between R and T state is not necessarily synchronized across the entire protein.[40] In 1969, Wyman[41] proposed such a model with "mixed conformations" (i.e. some protomers in the R state, some in the T state) for respiratory proteins in invertebrates.
Following a similar idea, the conformational spread model by Duke and colleagues[42] subsumes both the KNF and the MWC model as special cases. In this model, a subunit does not automatically change conformation upon ligand binding (as in the KNF model), nor do all subunits in a complex change conformations together (as in the MWC model). Conformational changes are stochastic with the likelihood of a subunit switching states depending on whether or not it is ligand bound and on the conformational state of neighbouring subunits. Thus, conformational states can "spread" around the entire complex.
Impact of upstream and downstream components on module's ultrasensitivity
In a living cell, ultrasensitive modules are embedded in a bigger network with upstream and downstream components. This components may constrain the range of inputs that the module will receive as well as the range of the module's outputs that network will be able to detect.[43] The sensitivity of a modular system is affected by these restrictions. The dynamic range limitations imposed by downstream components can produce effective sensitivities much larger than that of the original module when considered in isolation.
References
This article was adapted from the following source under a CC BY 4.0 license (2013) (reviewer reports):
Melanie I Stefan; {{cite journal}}
: CS1 maint: unflagged free DOI (link
- ^ Bohr C (1904). "Die Sauerstoffaufnahme des genuinen Blutfarbstoffes und des aus dem Blute dargestellten Hämoglobins". Zentralblatt Physiol. (in German). 23: 688–690.
- ^ .
- ^ Wyman J, Gill SJ (1990). Binding and linkage. Functional chemistry of biological molecules. Mill Valley: University Science Books.
- ^ Hill AV (1910). "The possible effects of the aggregation of the molecules of haemoglobin on its dissociation curves". J Physiol. 40: iv–vii.
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