Deletion (genetics)

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Deletion on a chromosome

In

nucleotides can be deleted, from a single base to an entire piece of chromosome.[1] Some chromosomes have fragile spots where breaks occur, which result in the deletion of a part of the chromosome. The breaks can be induced by heat, viruses, radiation, or chemical reactions. When a chromosome breaks, if a part of it is deleted or lost, the missing piece of chromosome is referred to as a deletion or a deficiency.[2]

For synapsis to occur between a chromosome with a large intercalary deficiency and a normal complete homolog, the unpaired region of the normal homolog must loop out of the linear structure into a deletion or compensation loop.

The smallest single base deletion mutations occur by a single base flipping in the template DNA, followed by template DNA strand slippage, within the DNA polymerase active site.[3][4][5]

Deletions can be caused by errors in

prokaryotic
organisms, such as bacteria.

Causes

Causes include the following:

Types

Types of deletion include the following:

  • Terminal deletion – a deletion that occurs towards the end of a chromosome.
  • Intercalary/interstitial deletion – a deletion that occurs from the interior of a chromosome.
  • Microdeletion – a relatively small amount of deletion (up to 5Mb that could include a dozen genes).

Micro-deletion is usually found in children with physical abnormalities. A large amount of deletion would result in immediate abortion (miscarriage).

Nomenclature

Three chromosomal abnormalities with ISCN nomenclature, with increasing complexity: (A) A tumour karyotype in a male with loss of the Y chromosome, (B) Prader–Willi Syndrome i.e. deletion in the 15q11-q12 region and (C) an arbitrary karyotype that involves a variety of autosomal and allosomal abnormalities.[6]
autosomal chromosome pairs, both the female (XX) and male (XY) versions of the two sex chromosomes, as well as the mitochondrial genome (at bottom left).

The

human chromosome nomenclature, which includes band names, symbols and abbreviated terms used in the description of human chromosome and chromosome abnormalities. Abbreviations include a minus sign (−) for chromosome deletions, and del for deletions of parts of a chromosome.[7]

Effects

Small deletions are less likely to be fatal; large deletions are usually fatal – there are always variations based on which genes are lost. Some medium-sized deletions lead to recognizable human disorders, e.g. Williams syndrome.

Deletion of a number of pairs that is not evenly divisible by three will lead to a

translation, producing a severely altered and potentially nonfunctional protein. In contrast, a deletion that is evenly divisible by three is called an in-frame deletion.[8]

Deletions are responsible for an array of genetic disorders, including some cases of male

SMN-encoding gene cause spinal muscular atrophy
, the most common genetic cause of infant death.

Microdeletions are associated with many different conditions, including Angelman Syndrome, Prader-Willi Syndrome, and DiGeorge Syndrome.[10] Some syndromes, including Angelman syndrome and Prader-Willi syndrome, are associated with both microdeletions and genomic imprinting, meaning that same microdeletion can cause two different syndromes depending on which parent the deletion came from.[11]

Recent work suggests that some deletions of highly conserved sequences (CONDELs) may be responsible for the evolutionary differences present among closely related species. Such deletions in humans, referred to as

chimpanzees and other varieties of mammals like ape or monkeys.[12]

Recent comprehensive patient-level classification and quantification of driver events in TCGA cohorts revealed that there are on average 12 driver events per tumor, of which 2.1 are deletions of tumor suppressors.[13]

Detection

The introduction of molecular techniques in conjunction with classical cytogenetic methods has in recent years greatly improved the diagnostic potential for chromosomal abnormalities. In particular, microarray-comparative genomic hybridization (CGH) based on the use of BAC clones promises a sensitive strategy for the detection of DNA copy-number changes on a genome-wide scale. The resolution of detection could be as high as >30,000 "bands" and the size of chromosomal deletion detected could as small as 5–20 kb in length.[14] Other computation methods were selected to discover DNA sequencing deletion errors such as end-sequence profiling.[15][16]

Mitochondrial DNA deletions

In the yeast

Rad52p and Rad59p encode proteins that are necessary for recombinational repair and are employed in the repair of double strand breaks in mitochondrial DNA.[17] Loss of these proteins decreases the rate of spontaneous DNA deletion events in mitochondria.[17]
This finding implies that the repair of DNA double-strand breaks by homologous recombination is a step in the formation of mitochondrial DNA deletions.

See also

References

  1. ^ .
  2. OCLC 880404074.{{cite book}}: CS1 maint: location missing publisher (link
    )
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  6. PMID 31228194.{{cite journal}}: CS1 maint: multiple names: authors list (link)
    - "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
    )"
  7. ^ "ISCN Symbols and Abbreviated Terms". Coriell Institute for Medical Research. Retrieved 2022-10-27.
  8. ^ LSDB — Controlled vocabulary terms Archived 2011-10-06 at the Wayback Machine at The GEN2PHEN Knowledge Centre. Posted Fri, 08/01/2010.
  9. .
  10. , retrieved 2022-01-07
  11. .
  12. .
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  14. .
  15. S2CID 2737235. Archived from the original
    (PDF) on 2020-05-31. Retrieved 2014-01-10.
  16. .
  17. ^ .