Dilution cloning
Dilution cloning or cloning by limiting dilution polyclonal mass of cells.
This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made. Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned.
After the final dilutions are produced,
References
- ^ ISBN 9780470528129.
- ^ ISBN 978047066658-6.
- PMID 21496350.
- PMID 20618588.
- S2CID 1433274.
- S2CID 26016732.
- – via ISEH.
External links
- Robert Staszewski "Cloning by Limiting Dilution: an improved estimate that an interesting culture is monoclonal" [1]
- John A. Ryan "Cell Cloning by Serial Dilution in 96 Well Plates".
- Vilma Maldonado,Jorge Meléndez-Zajgla "A modified method for cloning adherent mammalian cells".
- "Cloning by Limiting Dilution".[permanent dead link]
- "Cloning by limiting dilution".
- Nanci Donacki "Cloning by Limiting Dilution of Hybridoma".