Pyrimidine dimer
Pyrimidine dimers represent
While up to 100 such reactions per second may transpire in a
Types of pyrimidine dimers
Pyrimidine dimers encompass several types, each with distinct structures and implications for DNA integrity.
Cyclobutane pyrimidine dimer (CPD) is a dimer which features a four-membered ring formed by the fusion of two double-bonded carbons from adjacent pyrimidines. CPDs disrupt the formation of the
The 6–4 photoproduct (6–4 pyrimidine–pyrimidone, or 6–4 pyrimidine–pyrimidinone) is an alternate dimer configuration consisting of a single covalent bond linking the carbon at the 6 (C6) position of one pyrimidine ring and carbon at the 4 (C4) position of the adjoining base’s ring.[11] This type of conversion occurs at one third the frequency of CPDs and has a higher mutagenic risk.[12]
A third type of molecular lesion is a Dewar pyrimidinone, resulting from the reversible isomerization of a 6–4 photoproduct under further light exposure.[13]
Mutagenesis
Mutagenesis, the process of mutation formation, is significantly influenced by translesion
DNA repair
Pyrimidine dimers introduce local conformational changes in the
The UV dose that reduces a population of wild-type yeast cells to 37% survival is equivalent (assuming a Poisson distribution of hits) to the UV dose that causes an average of one lethal hit to each of the cells of the population.[18] The number of pyrimidine dimers induced per haploid genome at this dose was measured as 27,000.[18] A mutant yeast strain defective in the three pathways by which pyrimidine dimers were known to be repaired in yeast was also tested for UV sensitivity. It was found in this case that only one or, at most, two unrepaired pyrimidine dimers per haploid genome are lethal to the cell.[18] These findings thus indicate that the repair of thymine dimers in wild-type yeast is highly efficient.
Nucleotide excision repair, sometimes termed "dark reactivation", is a more general mechanism for repair of lesions and is the most common form of DNA repair for pyrimidine dimers in humans. This process works by using cellular machinery to locate the dimerized nucleotides and excise the lesion. Once the CPD is removed, there is a gap in the DNA strand that must be filled. DNA machinery uses the undamaged complementary strand to synthesize nucleotides off of and consequently fill in the gap on the previously damaged strand.[6]
Xeroderma pigmentosum (XP) is a rare genetic disease in humans in which genes that encode for NER proteins are mutated and result in decreased ability to combat pyrimidine dimers that form as a result of UV damage. Individuals with XP are also at a much higher risk of cancer than others, with a greater than 5,000 fold increased risk of developing skin cancers.[7] Some common features and symptoms of XP include skin discoloration, and the formation of multiple tumors proceeding UV exposure.
A few organisms have other ways to perform repairs:
- Spore photoproduct lyase is found in spore-forming bacteria. It returns thymine dimers to their original state.[19]
- bacteriophage T4. It is a base excision repair enzyme specific for pyrimidine dimers. It is then able to cut open the AP site.
Another type of repair mechanism that is conserved in humans and other non-mammals is
Effect of topical sunscreen and effect of absorbed sunscreen
Direct DNA damage is reduced by sunscreen, which also reduces the risk of developing a sunburn. When the sunscreen is at the surface of the skin, it filters the UV rays, which attenuates the intensity. Even when the sunscreen molecules have penetrated into the skin, they protect against direct DNA damage, because the UV light is absorbed by the sunscreen and not by the DNA.[21] Sunscreen primarily works by absorbing the UV light from the sun through the use of organic compounds, such as oxybenzone or avobenzone. These compounds are able to absorb UV energy from the sun and transition into higher-energy states. Eventually, these molecules return to lower energy states, and in doing so, the initial energy from the UV light can be transformed into heat. This process of absorption works to reduce the risk of DNA damage and the formation of pyrimidine dimers. UVA light makes up 95% of the UV light that reaches earth, whereas UVB light makes up only about 5%. UVB light is the form of UV light that is responsible for tanning and burning. Sunscreens work to protect from both UVA and UVB rays. Overall, sunburns exemplify DNA damage caused by UV rays, and this damage can come in the form of free radical species, as well as dimerization of adjacent nucleotides.[22]
See also
References
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- ^ "Structure of the major UV-induced photoproducts in DNA" (PDF). Expert reviews in molecular medicine. Cambridge University Press. 2 December 2002. Archived from the original (PDF) on 21 March 2005.
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