Ex vivo
Ex vivo (
A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or
Examples of ex vivo specimen use include:[citation needed]
- bioassays;
- using anticancer agents;
- measurements of optical and other tissue properties, especially in various environments that may not be life-sustaining (for example, at extreme pressures or temperatures);
- realistic models for surgical proceduredevelopment;
- investigations into the interaction of different energy types with tissues; or
- as phantoms in imaging technique development.
The term ex vivo means that the samples to be tested have been extracted from the organism. The term in vitro (lit. "within the glass") means the samples to be tested are obtained from a repository. In the case of cancer cells, a strain that would produce favorable results, then grown to produce a control sample and the number of samples required for the number of tests. These two terms are not synonymous even though the testing in both cases is "within the glass".
In cell biology, ex vivo procedures often involve living cells or tissues taken from an organism and cultured in a laboratory apparatus, usually under sterile conditions with no alterations, for up to 24 hours to obtain sufficient cells for the experiments. Experiments generally start after 24 hours of incubation. Using living cells or tissue from the same organism are still considered to be ex vivo. One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted on the CAM membrane of a chicken embryo outside the organism (chicken).[citation needed]