Fermentation

Source: Wikipedia, the free encyclopedia.
For other uses, see Fermentation (disambiguation).
Not to be confused with Anaerobic respiration.
Fermentation in progress: carbon dioxide bubbles form a froth on top of the fermentation mixture.

Fermentation is a metabolic process that produces chemical changes in organic substances through the action of enzymes. In biochemistry, it is broadly defined as the extraction of energy from carbohydrates in the absence of oxygen. In food production, it may more broadly refer to any process in which the activity of microorganisms brings about a desirable change to a foodstuff or beverage.[1] The science of fermentation is known as zymology.

In microorganisms, fermentation is the primary means of producing adenosine triphosphate (ATP) by the degradation of organic nutrients anaerobically.

Humans have used fermentation to produce foodstuffs and beverages since the Neolithic age. For example, fermentation is used for preservation in a process that produces lactic acid found in such sour foods as pickled cucumbers, kombucha, kimchi, and yogurt, as well as for producing alcoholic beverages such as wine and beer. Fermentation also occurs within the gastrointestinal tracts of all animals, including humans.[2]

Industrial fermentation is a broader term used for the process of applying microbes for the large-scale production of chemicals, biofuels, enzymes, proteins and pharmaceuticals.

Definitions and etymology

Below are some definitions of fermentation ranging from informal, general usages to more scientific definitions.[3]

  1. Preservation methods for food via microorganisms (general use).
  2. Any large-scale microbial process occurring with or without air (common definition used in industry, also known as industrial fermentation).
  3. Any process that produces alcoholic beverages or acidic dairy products (general use).
  4. Any energy-releasing metabolic process that takes place only under anaerobic conditions (somewhat scientific).
  5. Any metabolic process that releases energy from a sugar or other organic molecule, does not require oxygen or an electron transport system, and uses an organic molecule as the final electron acceptor (most scientific).

The word "ferment" is derived from the Latin verb fervere, which means to boil. It is thought to have been first used in the late 14th century in alchemy, but only in a broad sense. It was not used in the modern scientific sense until around 1600.[citation needed]

Biological role

Along with

aerobic respiration, fermentation is a method to extract energy from molecules. This method is the only one common to all bacteria and eukaryotes. It is therefore considered the oldest metabolic pathway, suitable for primeval environments – before plant life on Earth, that is, before oxygen in the atmosphere.[4]: 389  Nick Lane criticizes this proposal as the amount of energy released by fermentation is small, which can't lead to a thermodynamic driving force of prebiotic chemistry. The enzymes involved in fermentations, which are encoded by genes, could not have existed during prebiotic chemistry. In addition, he notes that the differences between the fermentation processes in archaea and bacteria indicate that fermentation likely evolved later on, developing independently in both types of primitive life.[5]

Yeast, a form of fungus, occurs in almost any environment capable of supporting microbes, from the skins of fruits to the guts of insects and mammals to the deep ocean. Yeasts convert (break down) sugar-rich molecules to produce ethanol and carbon dioxide.[6][7]

Basic mechanisms for fermentation remain present in all cells of higher organisms.

succinate and alanine.[9]
: 141 

Fermentative bacteria play an essential role in the production of

Archea) convert acetate to methane.[10]

Biochemical overview

pyruvate formed from sugar through glycolysis. The reaction produces oxidized NAD+ and an organic product, typical examples being ethanol, lactic acid, and hydrogen gas (H2), and often also carbon dioxide. However, more exotic compounds can be produced by fermentation, such as butyric acid and acetone. Fermentation products are considered waste products, since they cannot be metabolized further without the use of oxygen.[citation needed
]

Fermentation normally occurs in an

aerobic respiration as long as there is an adequate supply of sugars (a phenomenon known as the Crabtree effect).[13] Some fermentation processes involve obligate anaerobes, which cannot tolerate oxygen.[citation needed
]

Although

fermentation in the production of ethanol in beers, wines, and other alcoholic drinks, this is not the only possible agent: bacteria carry out the fermentation in the production of xanthan gum.[citation needed
]

Products of fermentation

Ethanol

Main article: Ethanol fermentation

In ethanol fermentation, one glucose molecule is converted into two ethanol molecules and two

sugar beets, produces ethanol that is added to gasoline.[19] In some species of fish, including goldfish and carp, it provides energy when oxygen is scarce (along with lactic acid fermentation).[20]

Before fermentation, a glucose molecule breaks down into two pyruvate molecules (glycolysis). The energy from this exothermic reaction is used to bind inorganic phosphates to ADP, which converts it to ATP, and convert NAD+ to NADH. The pyruvates break down into two acetaldehyde molecules and give off two carbon dioxide molecules as waste products. The acetaldehyde is reduced into ethanol using the energy and hydrogen from NADH, and the NADH is oxidized into NAD+ so that the cycle may repeat. The reaction is catalyzed by the enzymes pyruvate decarboxylase and alcohol dehydrogenase.[14]

History of bioethanol fermentation

The history of ethanol as a fuel spans several centuries and is marked by a series of significant milestones. Samuel Morey, an American inventor, was the first to produce ethanol by fermenting corn in 1826. However, it was not until the California Gold Rush in the 1850s that ethanol was first used as a fuel in the United States. Rudolf Diesel demonstrated his engine, which could run on vegetable oils and ethanol, in 1895, but the widespread use of petroleum-based diesel engines made ethanol less popular as a fuel. In the 1970s, the oil crisis reignited interest in ethanol, and Brazil became a leader in ethanol production and use. The United States began producing ethanol on a large scale in the 1980s and 1990s as a fuel additive to gasoline, due to government regulations. Today, ethanol continues to be explored as a sustainable and renewable fuel source, with researchers developing new technologies and biomass sources for its production.

  • 1826: Samuel Morey, an American inventor, was the first to produce ethanol by fermenting corn. However, ethanol was not widely used as a fuel until many years later. (1)
  • 1850s: Ethanol was first used as a fuel in the United States during the California Gold Rush. Miners used ethanol as a fuel for lamps and stoves because it was cheaper than whale oil. (2)
  • 1895: German engineer Rudolf Diesel demonstrated his engine, which was designed to run on vegetable oils, including ethanol. However, the widespread use of diesel engines fueled by petroleum made ethanol less popular as a fuel. (3)
  • 1970s: The oil crisis of the 1970s led to renewed interest in ethanol as a fuel. Brazil became a leader in ethanol production and use, due in part to government policies that encouraged the use of biofuels. (4)
  • 1980s–1990s: The United States began to produce ethanol on a large scale as a fuel additive to gasoline. This was due to the passage of the Clean Air Act in 1990, which required the use of oxygenates, such as ethanol, to reduce emissions. (5)
  • 2000s–present: There has been continued interest in ethanol as a renewable and sustainable fuel. Researchers are exploring new sources of biomass for ethanol production, such as switchgrass and algae, and developing new technologies to improve the efficiency of the fermentation process. (6)

Lactic acid

Main article: Lactic acid fermentation
See also: Mixed acid fermentation

Homolactic fermentation (producing only lactic acid) is the simplest type of fermentation. Pyruvate from glycolysis[21] undergoes a simple redox reaction, forming lactic acid.[22][23] Overall, one molecule of glucose (or any six-carbon sugar) is converted to two molecules of lactic acid:

C6H12O6 → 2 CH3CHOHCOOH

It occurs in the muscles of animals when they need energy faster than the

homolactic fermentation, where the end-product is mostly lactic acid, or heterolactic fermentation, where some lactate is further metabolized to ethanol and carbon dioxide[22] (via the phosphoketolase
pathway), acetate, or other metabolic products, e.g.:

C6H12O6 → CH3CHOHCOOH + C2H5OH + CO2

If lactose is fermented (as in yogurts and cheeses), it is first converted into glucose and galactose (both six-carbon sugars with the same atomic formula):

C12H22O11 + H2O → 2 C6H12O6

Heterolactic fermentation is in a sense intermediate between

alcoholic fermentation
. Reasons to go further and convert lactic acid into something else include:

Hydrogen gas

Main article: Fermentative hydrogen production

flatus.[citation needed
]

For example, Clostridium pasteurianum ferments glucose to butyrate, acetate, carbon dioxide, and hydrogen gas:[25] The reaction leading to acetate is:

C6H12O6 + 4 H2O → 2 CH3COO− + 2 HCO3− + 4 H+ + 4 H2

Other

Other types of fermentation include

butyrate fermentation, caproate fermentation, acetone–butanol–ethanol fermentation, and glyoxylate fermentation.[citation needed
]

In the broader sense

In food and industrial contexts, any chemical modification performed by a living being in a controlled container can be termed "fermentation". The following do not fall into the biochemical sense, but are called fermentation in the larger sense:

Alternative protein

Further information: List of fermented foods
Impossible Burger
.

Fermentation can be used to make alternative protein sources. It is commonly used to modify existing protein foods, including plant-based ones such as soy, into more flavorful forms such as

fermented tofu
.

More modern "fermentation" makes

egg substitutes. Some examples are:[26]

Heme proteins such as myoglobin and hemoglobin give meat its characteristic texture, flavor, color, and aroma. The myoglobin and leghemoglobin ingredients can be used to replicate this property, despite them coming from a vat instead of meat.[26][27]

Enzymes

Industrial fermentation can be used for enzyme production, where proteins with catalytic activity are produced and secreted by microorganisms. The development of fermentation processes, microbial strain engineering and recombinant gene technologies has enabled the commercialization of a wide range of enzymes. Enzymes are used in all kinds of industrial segments, such as food (lactose removal, cheese flavor), beverage (juice treatment), baking (bread softness, dough conditioning), animal feed, detergents (protein, starch and lipid stain removal), textile, personal care and pulp and paper industries.[28]

Modes of industrial operation

Most industrial fermentation uses batch or fed-batch procedures, although continuous fermentation can be more economical if various challenges, particularly the difficulty of maintaining sterility, can be met.[29]

Batch

In a batch process, all the ingredients are combined and the reactions proceed without any further input. Batch fermentation has been used for millennia to make bread and alcoholic beverages, and it is still a common method, especially when the process is not well understood.[30]: 1  However, it can be expensive because the fermentor must be sterilized using high pressure steam between batches.[29] Strictly speaking, there is often addition of small quantities of chemicals to control the pH or suppress foaming.[30]: 25 

Batch fermentation goes through a series of phases. There is a lag phase in which cells adjust to their environment; then a phase in which exponential growth occurs. Once many of the nutrients have been consumed, the growth slows and becomes non-exponential, but production of secondary metabolites (including commercially important antibiotics and enzymes) accelerates. This continues through a stationary phase after most of the nutrients have been consumed, and then the cells die.[30]: 25 

Fed-batch

See also: Fed-batch culture

Fed-batch fermentation is a variation of batch fermentation where some of the ingredients are added during the fermentation. This allows greater control over the stages of the process. In particular, production of secondary metabolites can be increased by adding a limited quantity of nutrients during the non-exponential growth phase. Fed-batch operations are often sandwiched between batch operations.[30]: 1 [31]

Open

The high cost of sterilizing the fermentor between batches can be avoided using various open fermentation approaches that are able to resist contamination. One is to use a naturally evolved mixed culture. This is particularly favored in wastewater treatment, since mixed populations can adapt to a wide variety of wastes.

Halophilic bacteria can produce bioplastics in hypersaline conditions. Solid-state fermentation adds a small amount of water to a solid substrate; it is widely used in the food industry to produce flavors, enzymes and organic acids.[29]

Continuous

In continuous fermentation, substrates are added and final products removed continuously.[29] There are three varieties: chemostats, which hold nutrient levels constant; turbidostats, which keep cell mass constant; and plug flow reactors in which the culture medium flows steadily through a tube while the cells are recycled from the outlet to the inlet.[31] If the process works well, there is a steady flow of feed and effluent and the costs of repeatedly setting up a batch are avoided. Also, it can prolong the exponential growth phase and avoid byproducts that inhibit the reactions by continuously removing them. However, it is difficult to maintain a steady state and avoid contamination, and the design tends to be complex.[29] Typically the fermentor must run for over 500 hours to be more economical than batch processors.[31]

History of the use of fermentation

Main article: Fermentation in food processing

The use of fermentation, particularly for

Christianity. The Baltic god Rugutis was worshiped as the agent of fermentation.[37][38]
In
symbolized by Capricorn
♑︎.

Louis Pasteur in his laboratory

In 1837, Charles Cagniard de la Tour, Theodor Schwann and Friedrich Traugott Kützing independently published papers concluding, as a result of microscopic investigations, that yeast is a living organism that reproduces by budding.[39][40]: 6  Schwann boiled grape juice to kill the yeast and found that no fermentation would occur until new yeast was added. However, a lot of chemists, including Antoine Lavoisier, continued to view fermentation as a simple chemical reaction and rejected the notion that living organisms could be involved. This was seen as a reversion to vitalism and was lampooned in an anonymous publication by Justus von Liebig and Friedrich Wöhler.[4]: 108–109 

The turning point came when Louis Pasteur (1822–1895), during the 1850s and 1860s, repeated Schwann's experiments and showed fermentation is initiated by living organisms in a series of investigations.[23][40]: 6  In 1857, Pasteur showed lactic acid fermentation is caused by living organisms.[41] In 1860, he demonstrated how bacteria cause souring in milk, a process formerly thought to be merely a chemical change. His work in identifying the role of microorganisms in food spoilage led to the process of pasteurization.[42]

In 1877, working to improve the French

brewing industry, Pasteur published his famous paper on fermentation, "Etudes sur la Bière", which was translated into English in 1879 as "Studies on fermentation".[43] He defined fermentation (incorrectly) as "Life without air",[44] yet he correctly showed how specific types of microorganisms cause specific types of fermentations and specific end-products.[citation needed
]

Although showing fermentation resulted from the action of living microorganisms was a breakthrough, it did not explain the basic nature of fermentation; nor did it prove it is caused by microorganisms which appear to be always present. Many scientists, including Pasteur, had unsuccessfully attempted to extract the fermentation enzyme from yeast.[44]

Success came in 1897 when the German chemist

Eduard Buechner ground up yeast, extracted a juice from them, then found to his amazement this "dead" liquid would ferment a sugar solution, forming carbon dioxide and alcohol much like living yeasts.[45]

Buechner's results are considered to mark the birth of biochemistry. The "unorganized ferments" behaved just like the organized ones. From that time on, the term enzyme came to be applied to all ferments. It was then understood fermentation is caused by enzymes produced by microorganisms.

Nobel Prize in chemistry for his work.[47]

Advances in microbiology and fermentation technology have continued steadily up until the present. For example, in the 1930s, it was discovered microorganisms could be

selection and hybridization developed as well, affecting most modern food fermentations.[citation needed
]

Post 1930s

The field of fermentation has been critical to the production of a wide range of consumer goods, from food and drink to industrial chemicals and pharmaceuticals. Since its early beginnings in ancient civilizations, the use of fermentation has continued to evolve and expand, with new techniques and technologies driving advances in product quality, yield, and efficiency. The period from the 1930s onward saw a number of significant advancements in fermentation technology, including the development of new processes for producing high-value products like antibiotics and enzymes, the increasing importance of fermentation in the production of bulk chemicals, and a growing interest in the use of fermentation for the production of functional foods and nutraceuticals.

The 1950s and 1960s saw the development of new fermentation technologies, such as the use of immobilized cells and enzymes, which allowed for more precise control over fermentation processes and increased the production of high-value products like antibiotics and enzymes.In the 1970s and 1980s, fermentation became increasingly important in the production of bulk chemicals like ethanol, lactic acid, and citric acid. This led to the development of new fermentation techniques and the use of genetically engineered microorganisms to improve yields and reduce production costs. In the 1990s and 2000s, there was a growing interest in the use of fermentation for the production of functional foods and nutraceuticals, which have potential health benefits beyond basic nutrition. This led to the development of new fermentation processes and the use of probiotics and other functional ingredients.

Overall, the period from 1930 onward saw significant advancements in the use of fermentation for industrial purposes, leading to the production of a wide range of fermented products that are now consumed around the world.

See also

Wikisource has the text of the 1911 Encyclopædia Britannica article "Fermentation".

References

  1. OCLC 52942889
    .
  2. ^ Bowen, Richard. "Microbial Fermentation". Hypertexts for biological sciences. Colorado State University. Retrieved 29 April 2018.
  3. ISBN 978-0-321-58202-7
    .
  4. ^
    ISBN 9780534406530
    .
  5. PMID 20108228
    .
  6. S2CID 35231385
    .
  7. PMID 17939990
    .
  8. ISBN 9781118139936
    .
  9. PMID 4913287. {{cite book}}: |journal= ignored (help
    )
  10. PMID 1512186
    .
  11. ISBN 978-0716720096
    .
  12. ISBN 978-0-07-255678-0
    .
  13. ISBN 9783642550133
    .
  14. ^
    ISBN 978-0-7167-9856-9
    .
  15. ISBN 978-0-7167-0174-3
    .
  16. PMID 9602932
    .
  17. PMID 20316063
    .
  18. ^ "Alcohol". Drugs.com. Retrieved 26 April 2018.
  19. ^ James Jacobs, Ag Economist. "Ethanol from Sugar". United States Department of Agriculture. Archived from the original on 2007-09-10. Retrieved 2007-09-04.
  20. ISBN 978-0-8493-4226-4
    .
  21. ISBN 978-0-534-46669-5
    . p. 86
  22. ^
    ISBN 978-0-07-147630-0
    . p. 61
  23. ^ a b A dictionary of applied chemistry, Volume 3. Thorpe, Sir Thomas Edward. Longmans, Green and Co., 1922. p.159
  24. ISBN 978-0-13-520875-5
    . Retrieved 2010-07-12.
  25. PMID 860983
    .
  26. ^ a b Flora Southey (27 January 2022). "What's next in alternative protein? 7 trends on the up in 2022". Food-Navigator.com, William Reed Business Media. Retrieved 27 January 2022.
  27. ISSN 1059-1028
    . Retrieved 2020-10-28.
  28. PMID 12323357
    .
  29. ^
    S2CID 21524147
    .
  30. ^
    ISBN 9780203911358
    .
  31. ^
    ISBN 9783527335152
    .
  32. PMID 15590771
    .
  33. S2CID 85577497
    .
  34. S2CID 7914033. 15008419. Archived from the original
    (PDF) on December 9, 2006. Retrieved 2007-01-28.
  35. ^ a b "Fermented fruits and vegetables. A global perspective". FAO Agricultural Services Bulletins - 134. Archived from the original on January 19, 2007. Retrieved 2007-01-28.
  36. ^ Dirar, H., (1993), The Indigenous Fermented Foods of the Sudan: A Study in African Food and Nutrition, CAB International, UK
  37. ^ "Gintaras Beresneviius. M. Strijkovskio Kronikos" lietuvi diev sraas". spauda.lt. Archived from the original on 2020-06-26. Retrieved 2013-10-27.
  38. ^ Rūgutis. Mitologijos enciklopedija, 2 tomas. Vilnius. Vaga. 1999. 293 p.
  39. ^ Shurtleff, William; Aoyagi, Akiko. "A Brief History of Fermentation, East and West". Soyinfo Center. Soyfoods Center, Lafayette, California. Retrieved 30 April 2018.
  40. ^
    ISBN 9783131084118
    .
  41. ^ Accomplishments of Louis Pasteur Archived 2010-11-30 at the Wayback Machine. Fjcollazo.com (2005-12-30). Retrieved on 2011-01-04.
  42. ^ HowStuffWorks "Louis Pasteur". Science.howstuffworks.com (2009-07-01). Retrieved on 2011-01-04.
  43. ^ Louis Pasteur (1879) Studies on fermentation: The diseases of beer, their causes, and the means of preventing them. Macmillan Publishers.
  44. ^ a b Modern History Sourcebook: Louis Pasteur (1822–1895): Physiological theory of fermentation, 1879. Translated by F. Faulkner, D.C. Robb.
  45. ISBN 978-84-370-3328-0
    . p. 25.
  46. ISBN 978-981-256-421-4
    . p. 7.
  47. ^ A treasury of world science, Volume 1962, Part 1. Runes, Dagobert David. Philosophical Library Publishers. 1962. p. 109.
  48. ISBN 9781351442510
    .
  49. doi:10.1111/j.1365-2621.1980.tb06534.x
    .

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