File:The Electrical Conductivity of Echinoderm Eggs, and Its Bearing on the Problems of Fertilisation and Artificial Parthenogenesis (1916) (14597982647).jpg

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Identifier: philtrans02035808 (find matches)
Title: The Electrical Conductivity of Echinoderm Eggs, and Its Bearing on the Problems of Fertilisation and Artificial Parthenogenesis
Year: 1916 (1910s)
Authors: Gray, J.
Subjects: Proceedings of the Royal Society of London Philosophical Transactions of the Royal Society
Publisher: Royal Society of London

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ds to bridge terminals; K, clamp for attachment to thermostat. AND ITS BEARING ON FERTILISATION AND ARTIFICIAL PARTHENOGENESIS. 485 to agree. It was found possible to collect the eggs in such a way as to obtainuniform readings from repeated observations. In later experiments the eggs weresettled by gentle use of a centrifuge ; such treatment does not hurt the eggs in anyway and greatly simplifies the technique. Whereas the above technique is sufficient for experiments with the eggs of Echinus,it was found to be unsatisfactory for the eggs of those sea urchins which wereavailable at Naples (Sphcer echinus, Strongylocentrotus, and Arbacia): If these eggsare settled by gravity it is impossible to obtain resistances of more than 25 ohmsowing to the presence of the wide gelatinous membranes which surround the eggs.It was found, however, that if the eggs are washed for a few minutes in slightly acidsea-water (100 c.c. sea-water + 5 c.c. N/10 HC1) the gelatinous membranes are entirelyTap Wa.
Text Appearing After Image:
Fig. 2.—Thermostat, with conductivity tube in position.W, worm tube ; S, sea-water ; K, kieselguhr; E, large earthenware bowl; T, thermometer. removed. Such treatment does not injure the eggs in any way, and greatly facilitatestheir subsequent manipulation for the following reasons :— 1. The eggs settle very much more quickly. 2. They are much closer together when settled and therefore give much higherresistances. 3. After fertilisation, no fertilisation membranes are formed. In the case ofSplicer echinus„ the surface of the egg does not appear to change after fertilisation untilafter the first hour of development. In the case of Strongylocentrotus the surfacemembrane of the egg becomes wrinkled, but no wide fertilisation membrane is formedif the washing with acid sea-water has been sufficient. 4. These eggs always settle quite uniformly in the conductivity tube, and successivedeterminations of their resistance give remarkably concordant results. 486 ME. J. GRAY ON THE ELECTRICAphiltrans02035808

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  • bookid:philtrans02035808
  • bookyear:1916
  • bookdecade:1910
  • bookcentury:1900
  • bookauthor:Gray__J_
  • booksubject:Proceedings_of_the_Royal_Society_of_London
  • booksubject:Philosophical_Transactions_of_the_Royal_Society
  • bookpublisher:Royal_Society_of_London
  • bookcontributor:
  • booksponsor:
  • bookleafnumber:4
  • bookcollection:philosophicaltransactions
  • bookcollection:additional_collections
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30 July 2014



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current13:33, 19 August 2015Thumbnail for version as of 13:33, 19 August 20152,802 × 1,916 (861 KB)Fæ== {{int:filedesc}} == {{information |description={{en|1=<br> '''Identifier''': philtrans02035808 ([https://commons.wikimedia.org/w/index.php?title=Special%3ASearch&profile=default&fulltext=Search&search=insource%3A%2Fphiltrans02035808%2F find matches]...
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