Gene bank
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Gene banks are a type of
In plants, it is possible to thaw the material and propagate it. However, in animals, a living female is required for artificial insemination. While it is often difficult to use frozen animal sperm and eggs, there are many examples of it being done successfully.
In an effort to conserve
The database of the largest gene banks in the world can be queried via a common website, Genesys. A number of global gene banks are coordinated by the CGIAR Genebank Platform
Types of gene bank
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Seed bank
A
In vitro bank
In this technique, buds, protocorm and meristematic cells are preserved through particular light and temperature arrangements in a nutrient medium, which is either a gel or in liquid form. This technique is used to preserve seedless plants and plants that reproduce asexually or that require preservation as clones such as commercial cultivars.[6]
Cryobank
In this technique, a seed or embryo is preserved at very low temperatures. It is usually preserved in liquid nitrogen at -196 °C. The storage of pollen is where pollen grains are stored through a cryopreservation technique called vitrification. Vitrification is the prosses where pollen grains are frozen but no ice or ice crystals form.[8] The pollen, which is stored in liquid nitrogen, is kept at temperatures of -180 °C to -196 °C. The National Seed Storage Lab in Fort Collins, Colorado currently uses this technique to store pollen.[11] Pollen can also be freeze dried and stored at temperatures of 5 °C to -18 °C.[4] An important element that must be considered is the levels of moisture in the pollen. If the pollen grains have a low moisture content it helps increase the length of the pollen’s life. Low levels of moisture help the pollen freeze without creating ice or ice crystals, which helps preserve the life span of the pollen while it is being stored.[12][13] Ideal levels of moisture content in the pollen depends on the type of plant. The pollen from different plant species can be put into two groups. One is Binucleate pollen, which has a thicker exine and the second is Trinucleate pollen, which has a thinner exine. Binucleate pollen has a higher lifespan when frozen at a low moisture level. Trinucleate pollen, however, has a lower lifespan when frozen at a low moisture level.[12] Some ways that scientists decrease moisture level is to expose the pollen to diluted salt solutions, silica gel, dry air, or treatment with vitrification solutions.[14]
This is a method of sowing plants for the conservation of genes. For this purpose, an ecosystem is created artificially. Through this method, one can compare the differences among plants of different species and can study them in detail. It needs more land, adequate soil, weather, etc. Germplasm of important crops are conserved through this method. 42,000 varieties of rice are conserved in the Central Rice Research Institute in Orissa.[citation needed][15]
Storage of pollen
Field gene bank
See also
References
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