Klenow fragment
The Klenow fragment is a large
The other smaller fragment formed when DNA polymerase I from
Research
Because the 5' → 3' exonuclease activity of DNA polymerase I from
- Synthesis of double-stranded DNA from single-stranded templates
- Filling in receded 3' ends of DNA fragments to make 5' overhang blunt
- Digesting away protruding 3' overhangs
- Preparation of DNA probes
The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process,[2] before being replaced by thermostable enzymes such as Taq polymerase.[3]
The exo- Klenow fragment
Just as the 5' → 3'
The exo-Klenow fragment is used in some fluorescent labeling reactions for microarray, and also in dA and dT tailing, an important step in the process of ligating DNA adapters to DNA fragments, frequently used in preparing DNA libraries for Next-Gen sequencing. (for instance see [1])
References
External links
- Klenow+Fragment at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
- Diagram at vivo.colostate.edu