Levamisole

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Levamisole
Skeletal formula of levamisole
Ball-and-stick model of the levamisole molecule
Clinical data
Trade namesDecaris, Ergamisol
AHFS/Drugs.comMicromedex Detailed Consumer Information
MedlinePlusa697011
License data
QP52AE01 (WHO)
Legal status
Legal status
Pharmacokinetic data
MetabolismLiver
Elimination half-life3–4 hours
ExcretionKidney (70%)
Identifiers
  • (S)-6-Phenyl-2,3,5,6-tetrahydroimidazo[2,1-b] [1,3]thiazole
JSmol)
Density1.31 g/cm3
Melting point60 °C (140 °F)
Solubility in waterhydrochloride: 210 mg/mL (20 °C)
  • N\2=C1/SCCN1C[C@@H]/2c3ccccc3
  • InChI=1S/C11H12N2S/c1-2-4-9(5-3-1)10-8-13-6-7-14-11(13)12-10/h1-5,10H,6-8H2/t10-/m1/s1 checkY
  • Key:HLFSDGLLUJUHTE-SNVBAGLBSA-N checkY
 ☒NcheckY (what is this?)  (verify)

Levamisole, sold under the brand name Ergamisol among others, is a medication used to treat parasitic worm infections, specifically ascariasis and hookworm infections.[1] It is taken by mouth.[2]

Side effects may include abdominal pain, vomiting, headache, and dizziness.

third trimester of pregnancy.[2] Serious side effects may include an increased risk of infection.[3] It belongs to the anthelmintic class of medications.[3]

Levamisole was invented in 1966 in

dewormer for cattle.[6][7]

Medical uses

Worms

Levamisole was originally used as an

roundworm infestations in freshwater tropical fish.[9]

Other

Levamisole has been used to treat a variety of dermatologic conditions, including skin infections,

aphthous ulcers.[10]

An interesting side effect these reviewers reported in passing was "neurologic excitement". Later papers, from the Janssen group and others, indicate levamisole and its enantiomer, dexamisole, have some mood-elevating or antidepressant properties, although this was never a marketed use of the drug.[11][12]

Adverse effects

One of the more serious side effects of levamisole is agranulocytosis, or the depletion of the white blood cells. In particular, neutrophils appear to be affected the most. This occurs in 0.08–5% of the studied populations.[13]

It has been used as an

erythematous painful papules can appear almost anywhere on skin.[14][15][16]

Metabolism

Levamisole is readily absorbed from the gastrointestinal tract and metabolized in the liver. Its time to peak plasma concentration is 1.5–2 hours. The plasma elimination half-life is fairly quick at 3–4 hours which can contribute to not detecting levamisole intoxication. The metabolite half-life is 16 hours. Levamisole's excretion is primarily through the kidneys, with about 70% being excreted over 3 days. Only about 5% is excreted as unchanged levamisole.[17][18]

Drug testing of racehorse urine has led to the revelation that among levamisole equine metabolites are both pemoline and aminorex, stimulants that are forbidden by racing authorities.[19][20][21] Further testing confirmed aminorex in human and canine urine, meaning that both humans and dogs also metabolize levamisole into aminorex,[22] though it is unclear whether plasma aminorex is present at any appreciable level. Blood samples following oral administration of levamisole out to 172 hr post-dose did not demonstrate any plasma aminorex levels above that of the limit of quantification (LoQ). Additionally, in cocaine-positive plasma samples, of which 42% contained levamisole, aminorex was never reported at concentrations higher than LoQ.[23]

Detection in body fluids

Levamisole may be quantified in blood, plasma, or urine as a diagnostic tool in clinical poisoning situations or to aid in the medicolegal investigation of suspicious deaths involving adulterated street drugs. About 3% of an oral dose is eliminated unchanged in the 24-hour urine of humans. A post mortem blood levamisole concentration of 2.2 mg/L was present in a woman who died of a cocaine overdose.[24][25]

Adulterant in illegal drugs

Levamisole has increasingly been used as a

cutting agent in cocaine sold around the globe with the highest incidence being in the United States. In 2008–2009, levamisole was found in 69% of cocaine samples seized by the Drug Enforcement Administration (DEA).[14] By April 2011, the DEA reported the adulterant was found in 82% of seizures.[26]

Levamisole adds bulk and weight to powdered cocaine (whereas other adulterants produce smaller "rocks" of cocaine) and makes the drug appear purer.[27] In a series of investigative articles for The Stranger, Brendan Kiley details other rationales for levamisole's rise as an adulterant: possible stimulant effects, a similar appearance to cocaine, and an ability to pass street purity tests.[28]

Levamisole suppresses the production of white blood cells, resulting in neutropenia and agranulocytosis. With the increasing use of levamisole as an adulterant, a number of these complications have been reported among cocaine users.[14][29][30] Levamisole has also been linked to a risk of vasculitis,[31] and two cases of vasculitic skin necrosis have been reported in users of cocaine adulterated with levamisole.[32]

Levamisole-tainted cocaine has caused three deaths and sickened over 100 in US and Canada, as of 2009.[33]

Chemistry

The original synthesis at Janssen Pharmaceutica resulted in the preparation of a

enantiomers, whose hydrochloride salt was reported to have a melting point of 264–265 °C; the free base of the racemate has a melting point of 87–89 °C. The racemic mixture is referred to as "tetramisole" - levamisole refers only to the levorotatory enantiomer of tetramisole.[citation needed
]

Toxicity

The

LD50 (intravenous, mouse) is 22 mg/kg.[34]

Laboratory use

Levamisole

isoforms of alkaline phosphatase (e.g., human liver, bone, kidney, and spleen) except the intestinal and placental isoform.[35][36] It is thus used as an inhibitor along with substrate to reduce background alkaline phosphatase activity in biomedical assays involving detection signal amplification by intestinal alkaline phosphatase, for example in in situ hybridization or Western blot protocols.[citation needed
]

It is used to immobilize the nematode C. elegans on glass slides for imaging and dissection.[37]

In a C. elegans behavioral assay, analyzing the time course of paralysis provides information about the neuromuscular junction. Levamisole acts as an acetylcholine receptor agonist, which leads to muscle contraction. Continuing activation leads to paralysis. The time course of paralysis provides information about the acetylcholine receptors on the muscle. For example, mutants with fewer acetylcholine receptors may paralyze slower than wild type.[38]

Research

It has been studied as a method to stimulate the immune system as part of the treatment of cancer.[39] It has also shown some efficacy in the treatment of nephrotic syndrome in children.[40]

After being pulled from the market in the US and Canada in 1999 and 2003, respectively, levamisole has been tested in combination with

tetramisole showed similar effect.[41]

Veterinary uses

The combination

indicated for the treatment and control of gastrointestinal roundworms, lungworms, grubs, sucking lice, and mange mites in cattle.[6] It is given by subcutaneous injection.[6]

References

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  3. ^ a b "Levamisole Advanced Patient Information - Drugs.com". www.drugs.com. Archived from the original on 20 December 2016. Retrieved 8 December 2016.
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    . WHO/MVP/EMP/IAU/2019.06. License: CC BY-NC-SA 3.0 IGO.
  6. ^ a b c "Animal Drugs @ FDA". animaldrugsatfda.fda.gov. Retrieved 25 January 2023.
  7. ISBN 9781119073673. Archived
    from the original on 10 September 2017.
  8. ^ "Levamisole". Martindale: The Complete Drug Reference. Lexicomp. Archived from the original on 21 December 2016. Retrieved 21 April 2014.
  9. ^ Sanford S (2007). "Levamisole Hydrochloride: Its application and usage in freshwater aquariums". Loaches Online. Archived from the original on 1 March 2009. Retrieved 27 February 2009.
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  16. ^ "Cocaine powder: review of its prevalence, patterns of use and harm". Advisory Council on the Misuse of Drugs. 12 March 2015.
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  25. ^ Baselt R (2011). Disposition of Toxic Drugs and Chemicals in Man (PDF) (9th ed.). Seal Beach, CA: Biomedical Publications. pp. 901–902. Archived from the original (PDF) on 10 September 2011. Retrieved 22 January 2011.
  26. ^ Moisse K (23 June 2011). "Cocaine Laced With Veterinary Drug Levamisole Eats Away at Flesh". ABC News. Archived from the original on 25 June 2011. Retrieved 23 June 2011.
  27. ^ Doheny K (1 June 2010). "Contaminated Cocaine Can Cause Flesh to Rot". Yahoo!. Archived from the original on 7 June 2010. Retrieved 8 June 2010.
  28. ^ Kiley B (17 August 2010). "The Mystery of the Tainted Cocaine". The Stranger. Archived from the original on 11 December 2010. Retrieved 21 December 2010.
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