Ligand (biochemistry)
In
Binding occurs by
Ligand binding to a
Receptor/ligand binding affinity
The interaction of ligands with their binding sites can be characterized in terms of a binding affinity. In general, high-affinity ligand binding results from greater attractive forces between the ligand and its receptor while low-affinity ligand binding involves less attractive force. In general, high-affinity binding results in a higher occupancy of the receptor by its ligand than is the case for low-affinity binding; the residence time (lifetime of the receptor-ligand complex) does not correlate. High-affinity binding of ligands to receptors is often physiologically important when some of the binding energy can be used to cause a conformational change in the receptor, resulting in altered behavior for example of an associated ion channel or enzyme.
A ligand that can bind to and alter the function of the receptor that triggers a physiological response is called a receptor agonist. Ligands that bind to a receptor but fail to activate the physiological response are receptor antagonists.
Agonist binding to a receptor can be characterized both in terms of how much physiological response can be triggered (that is, the
Low-affinity binding (high Ki level) implies that a relatively high concentration of a ligand is required before the binding site is maximally occupied and the maximum physiological response to the ligand is achieved. In the example shown to the right, two different ligands bind to the same receptor binding site. Only one of the agonists shown can maximally stimulate the receptor and, thus, can be defined as a full agonist. An agonist that can only partially activate the physiological response is called a partial agonist. In this example, the concentration at which the full agonist (red curve) can half-maximally activate the receptor is about 5 x 10−9 Molar (nM = nanomolar).
Binding affinity is most commonly determined using a
For the use of statistical mechanics in a quantitative study of the ligand-receptor binding affinity, see the comprehensive article[8] on the configurational partition function.
Drug or hormone binding potency
Binding affinity data alone does not determine the overall potency of a drug or a naturally produced (biosynthesized) hormone.[9]
Potency is a result of the complex interplay of both the binding affinity and the ligand efficacy.[9]
Drug or hormone binding efficacy
Ligand efficacy refers to the ability of the ligand to produce a biological response upon binding to the target receptor and the quantitative magnitude of this response. This response may be as an agonist, antagonist, or inverse agonist, depending on the physiological response produced.[10]
Selective and non-selective
Selective ligands have a tendency to bind to very limited kinds of receptor, whereas non-selective ligands bind to several types of receptors. This plays an important role in
Hydrophobic ligands
For hydrophobic ligands (e.g. PIP2) in complex with a hydrophobic protein (e.g. lipid-gated ion channels) determining the affinity is complicated by non-specific hydrophobic interactions. Non-specific hydrophobic interactions can be overcome when the affinity of the ligand is high.[11] For example, PIP2 binds with high affinity to PIP2 gated ion channels.
Bivalent ligand
Bivalent ligands consist of two drug-like molecules (pharmacophores or ligands) connected by an inert linker. There are various kinds of bivalent ligands and are often classified based on what the pharmacophores target. Homobivalent ligands target two of the same receptor types. Heterobivalent ligands target two different receptor types.[12] Bitopic ligands target an orthosteric binding sites and allosteric binding sites on the same receptor.[13] In scientific research, bivalent ligands have been used to study
Bivalent ligands usually tend to be larger than their monovalent counterparts, and therefore, not 'drug-like' as in Lipinski's
Mono- and polydesmic ligands
Ligands of proteins can be characterized also by the number of protein chains they bind. "Monodesmic" ligands (μόνος: single, δεσμός: binding) are ligands that bind a single protein chain, while "polydesmic" ligands (πολοί: many) [31] are frequent in protein complexes, and are ligands that bind more than one protein chain, typically in or near protein interfaces. Recent research shows that the type of ligands and binding site structure has profound consequences for the evolution, function, allostery and folding of protein compexes.[32][33]
Privileged scaffold
A privileged scaffold[34] is a molecular framework or chemical moiety that is statistically recurrent among known drugs or among a specific array of biologically active compounds. These privileged elements[35] can be used as a basis for designing new active biological compounds or compound libraries.
Methods used to study binding
Main methods to study protein–ligand interactions are principal hydrodynamic and calorimetric techniques, and principal spectroscopic and structural methods such as
- Fourier transform spectroscopy
- Raman spectroscopy
- Fluorescence spectroscopy
- Circular dichroism
- Nuclear magnetic resonance
- Mass spectrometry
- Atomic force microscope
- Paramagneticprobes
- Dual polarisation interferometry
- Multi-parametric surface plasmon resonance
- Ligand binding assay and radioligand binding assay
Other techniques include: fluorescence intensity, bimolecular fluorescence complementation, FRET (fluorescent resonance energy transfer) / FRET quenching surface plasmon resonance, bio-layer interferometry, Coimmunopreciptation indirect ELISA, equilibrium dialysis, gel electrophoresis, far western blot, fluorescence polarization anisotropy, electron paramagnetic resonance, microscale thermophoresis, switchSENSE.
The dramatically increased computing power of supercomputers and personal computers has made it possible to study protein–ligand interactions also by means of
See also
- Agonist
- Schild regression
- Allosteric regulation
- Ki Database
- Docking@Home
- GPUGRID.net
- DNA binding ligand
- BindingDB
- SAMPL Challenge
References
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- ^ "The difference between Ki, Kd, IC50, and EC50 values". The Science Snail. 31 December 2019.
- ^ See Homologous competitive binding curves Archived 2007-12-19 at the Wayback Machine, A complete guide to nonlinear regression, curvefit.com.
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- "A hot road to new drugs". Phys.org. February 24, 2010.
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- ^ Vu-Quoc, L., Configuration integral (statistical mechanics), 2008. this wiki site is down; see this article in the web archive on 2012 April 28.
- ^ a b https://doi.org/10.1093/oso/9780199744121.003.0006
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