Molecular mass
The molecular mass (m) is the mass of a given
The molecular mass and relative molecular mass are distinct from but related to the
The definition of molecular weight is most authoritatively synonymous with relative molecular mass; however, in common practice, use of this terminology is highly variable. When the molecular weight is given with the unit Da, it is frequently as a weighted average similar to the molar mass but with different units. In molecular biology, the mass of macromolecules is referred to as their molecular weight and is expressed in kDa, although the numerical value is often approximate and representative of an average.
The terms "molecular mass", "molecular weight", and "molar mass" may be used interchangeably in less formal contexts where unit- and quantity-correctness is not needed. The molecular mass is more commonly used when referring to the mass of a single or specific well-defined molecule and less commonly than molecular weight when referring to a weighted average of a sample. Prior to the
The molecular mass of small to medium size molecules, measured by mass spectrometry, can be used to determine the
Calculation
Molecular masses are calculated from the
216O) and 22.027 7364(9) Da (2H
218O).
Atomic and molecular masses are usually reported in
Relative molecular mass of CH4 | |||
---|---|---|---|
Standard atomic weight | Number | Total molecular weight (dimensionless) | |
C | 12.011 | 1 | 12.011 |
H | 1.008 | 4 | 4.032 |
CH4 | 16.043 | ||
Molecular mass of 12C1H4 | |||
Nuclide mass | Number | Total molecular mass (Da or u) | |
12C | 12.00 | 1 | 12.00 |
1H | 1.007825 | 4 | 4.0313 |
CH4 | 16.0313 |
The uncertainty in molecular mass reflects variance (error) in measurement not the natural variance in isotopic abundances across the globe. In high-resolution mass spectrometry the mass isotopomers 12C1H4 and 13C1H4 are observed as distinct molecules, with molecular masses of approximately 16.031 Da and 17.035 Da, respectively. The intensity of the mass-spectrometry peaks is proportional to the isotopic abundances in the molecular species. 12C 2H 1H3 can also be observed with molecular mass of 17 Da.
Determination
Mass spectrometry
In mass spectrometry, the molecular mass of a small molecule is usually reported as the monoisotopic mass, that is, the mass of the molecule containing only the most common isotope of each element. This also differs subtly from the molecular mass in that the choice of isotopes is defined and thus is a single specific molecular mass of the many possibilities. The masses used to compute the monoisotopic molecular mass are found on a table of isotopic masses and are not found on a typical periodic table. The average molecular mass is often used for larger molecules since molecules with many atoms are unlikely to be composed exclusively of the most abundant isotope of each element. A theoretical average molecular mass can be calculated using the standard atomic weights found on a typical periodic table, since there is likely to be a statistical distribution of atoms representing the isotopes throughout the molecule. The average molecular mass of a sample, however, usually differs substantially from this since a single sample average is not the same as the average of many geographically distributed samples.
Mass photometry
Mass photometry (MP) is a rapid, in-solution, label-free method of obtaining the molecular mass of proteins, lipids, sugars & nucleic acids at the single-molecule level. The technique is based on interferometric scattered light microscopy. Mass photometry can measure molecular mass to an accurate degree over a wide range of molecular masses (40kDa – 5MDa).
Hydrodynamic methods
To a first approximation, the basis for determination of molecular mass according to
Static light scattering
It is also possible to determine absolute molecular mass directly from light scattering, traditionally using the
See also
- Cryoscopy and cryoscopic constant
- Ebullioscopy and ebullioscopic constant
- Dumas method of molecular weight determination
- François-Marie Raoult
- Standard atomic weight
- Mass number
- Absolute molar mass
- Molar mass distribution
- Dalton (unit)
- SDS-PAGE
References
- ISBN 978-92-822-2272-0
- ^ Mohr, Peter J.; Taylor, Barry N.; Newell, David B. (2011). "CODATA Recommended Values of the Fundamental Physical Constants: 2010". Database developed by J. Baker, M. Douma, and S. Kotochigova. National Institute of Standards and Technology, Gaithersburg, MD 20899.
- ^ IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). Online version (2019-) created by S. J. Chalk. ISBN 0-9678550-9-8. https://doi.org/10.1351/goldbook.
- NIST. Retrieved 2007-10-14.
- ^ Young et al. (2018). Quantitative imaging of single biological macromolecules. Science 360, 423-427. DOI: https://doi.org/10.1126/science.aar5839
- ^ Sonn-Segev, A., Belacic, K., Bodrug, T. et al. Quantifying the heterogeneity of macromolecular machines by mass photometry. Nat Commun 11, 1772 (2020). https://doi.org/10.1038/s41467-020-15642-w
- ^ Soltermman et al. Quantifying protein-protein interactions by molecular counting using mass photometry. Angew. Chem Int Ed, 2020, 59(27), 10774-10779
- ^ Paul, Hiemenz C., and Lodge P. Timothy. Polymer Chemistry. Second ed. Boca Raton: CRC P, 2007. 336, 338–339.
- .
- PMID 29142693.
External links
- A Free Android application for molecular and reciprocal weight calculation of any chemical formula
- Stoichiometry Add-In for Microsoft Excel Archived 2011-05-11 at the Wayback Machine for calculation of molecular weights, reaction coefficients and stoichiometry.