Nucleomorph

This article is missing information about inclusions including nucleolus (plus rDNA!), division behavior, mentioned in refs of doi:10.1007/s00709-017-1153-5 (RG link). Please expand the article to include this information. Further details may exist on the talk page. (October 2021)

Diagram of a four membraned chloroplast containing a nucleomorph.

Nucleomorphs are small, vestigial eukaryotic

Of the two known plastids that contain nucleomorphs, both have four membranes, the nucleomorph residing in the periplastidial compartment, evidence of being engulfed by a eukaryote through phagocytosis.^[1]

In addition, some species within the dinoflagellates that have gone through tertiary endosymbiosis also have endosymbionts with both a nucleus and mitochondria present.^[5]

Nucleomorph genome

Nucleomorphs represent some of the smallest genomes ever sequenced. After the red or green alga was engulfed by a

The model cryptomonad Guillardia theta became an important focus for scientists studying nucleomorphs. Its complete nucleomorph sequence was published in 2001, coming in at 551 Kbp. The G. theta sequence gave insight as to what genes were retained in nucleomorphs. Most of the genes that moved to the host cell involved protein synthesis, leaving behind a compact genome with mostly single-copy “housekeeping” genes (affecting transcription, translation, protein folding and degradation and splicing) and no mobile elements. The genome contains 513 genes, 465 of which code for protein. Thirty genes are considered “plastid” genes, coding for plastid proteins.^[1][7]

The genome sequence of another organism, the chlorarachniophyte Bigelowiella natans indicates that its nucleomorph is probably the vestigial nucleus of a green alga, whereas the nucleomorph in G. theta probably came from a red alga. The B. natans genome is smaller than that of G. theta, with about 373 Kbp and contains 293 protein-coding genes as compared to the 465 genes in G. theta. B. natans also only has 17 genes that code for plastid proteins, again fewer than G. theta. Comparisons between the two organisms have shown that B. natans contains significantly more introns (852) than G. theta (17). B. natans also had smaller introns, ranging from 18-21 bp, whereas G. theta’s introns ranged from 42-52 bp.^[1]

Both the genomes of B. natans and G. theta display evidence of genome reduction besides elimination of genes and tiny size, including elevated composition of adenine (A) and thymine (T), and high substitution rates.^[4][7][8]

Persistence of nucleomorphs

There are no recorded instances of vestigial nuclei in any other secondary plastid-containing organisms, yet they have been retained independently in the cryptomonads and chlorarachniophytes. Plastid gene transfer happens frequently in many organisms, and it is unusual that these nucleomorphs have not disappeared entirely. One theory as to why these nucleomorphs have not disappeared as they have in other groups is that

Nucleomorphs also often code for many of their own critical functions, like transcription and translation.^[9] Some say that as long as there exists a gene in the nucleomorph that codes for proteins necessary for the plastid’s functioning that are not produced by the host cell, the nucleomorph will persist.^[1] In cryptophytes and chlorarachniophytes all DNA transfer between the nucleomorph and host genome seems to have ceased, but the process is still going on in a few dinoflagellates.^[10]

See also

• Endosymbiont

References

Guillardia theta: 54;
Rhodomonas salina: 18;
Cryptomonas sp.: 15;
Chlorarachniophyceae sp.:10;
Cryptomonas paramecium: 9;
Cryptomonas erosa: 7.