Nylon-eating bacteria

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Nylon-eating bacteria
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Actinomycetota
Class: Actinomycetia
Order: Micrococcales
Family: Micrococcaceae
Genus: Paenarthrobacter
Species:
P. ureafaciens
Variety:
P. u. var. KI72
Trinomial name
Paenarthrobacter ureafaciens var. KI72
GTDB r95 & NCBI, 2020 (Busse HJ, 2016)
Synonyms
  • Arthrobacter sp. KI72
    Takehara I, 2017[1]
  • Flavobacterium sp. KI72
    Negoro S, 1980
  • Achromobacter guttatus KI72
    Kinoshita S, 1975

(Due to an OCR error, the strain name has occasionally been reported as "K172".)

Paenarthrobacter ureafaciens KI72, popularly known as nylon-eating bacteria, is a strain of Paenarthrobacter ureafaciens that can digest certain by-products of nylon 6 manufacture.[2] It uses a set of enzymes to digest nylon, popularly known as nylonase.[3]

Discovery and nomenclature

Chemical structure of 6-aminohexanoic acid

In 1975, a team of Japanese scientists discovered a strain of bacterium, living in ponds containing waste water from a nylon factory, that could digest certain byproducts of nylon 6 manufacture, such as the linear dimer of 6-aminohexanoate. These substances are not known to have existed before the invention of nylon in 1935. It was initially named as Achromobacter guttatus.[4]

Studies in 1977 revealed that the three enzymes that the bacteria were using to digest the byproducts were significantly different from any other enzymes produced by any other bacteria, and not effective on any material other than the manmade nylon byproducts.[5]

The bacterium was reassigned to Flavobacterium in 1980.[6] Its genome was resolved in 2017, again reassigning it to Arthrobacter.[1] The Genome Taxonomy Database considers it a strain of Paenarthrobacter ureafaciens following a 2016 reclassification.[7] As of January 2021, the NCBI taxonomy browser has been updated to match GTDB.

Descendant strains

A few newer strains have been created by growing the original KI72 in different conditions, forcing it to adapt. These include KI722, KI723, KI723T1, KI725, KI725R, and many more.[8]

The enzymes

The bacterium contains the following three enzymes:

All three enzymes are encoded on a

E. coli, as shown in a 1983 publication.[10]

EI

The enzyme EI is related to amidases. Its structure was resolved in 2010.[11]

EII

EII has evolved by gene duplication followed by base substitution of another protein EII'. Both enzymes have 345 identical aminoacids out of 392 aminoacids (88% homology). The enzymes are similar to beta-lactamase.[12]

The EII' (NylB', P07062) protein is about 100x times less efficient compared to EII. A 2007 research by the Seiji Negoro team shows that just two amino-acid alterations to EII', i.e. G181D and H266N, raises its activity to 85% of EII.[9]

EIII

The structure of EIII was resolved in 2018. Instead of being a completely novel enzyme, it appears to be a member of the N-terminal nucleophile (N-tn) hydrolase family.[13] Specifically, computational approaches classify it as a MEROPS S58 (now renamed P1) hydrolase. The protein is expressed as a precursor, which then cleaves itself into two chains.[14][15] Outside of this plasmid, > 95% similar proteins are found in Agromyces and Kocuria.[13]

EIII was originally thought to be completely novel. Susumu Ohno proposed that it had come about from the combination of a gene-duplication event with a frameshift mutation. An insertion of thymidine would turn an arginine-rich 427aa protein into this 392aa enzyme.[16]

Role in evolution teaching

Main article: Nylon-eating bacteria and creationism

There is scientific consensus that the capacity to synthesize nylonase most probably developed as a single-step mutation that survived because it improved the fitness of the bacteria possessing the mutation. More importantly, one of the enzymes involved was produced by a

frame-shift mutation that completely scrambled existing genetic code data.[17] Despite this, the new gene still had a novel, albeit weak, catalytic capacity. This is seen as a good example of how mutations easily can provide the raw material for evolution by natural selection.[18][19][20][21]

A 1995 paper showed that scientists have also been able to induce another species of bacterium, Pseudomonas aeruginosa, to evolve the capability to break down the same nylon byproducts in a laboratory by forcing them to live in an environment with no other source of nutrients.[22]

See also

References

  1. ^
    PMID 28450506
    .
  2. S2CID 20206702
    .
  3. ^ Michael Le Page (March 2009). "Five classic examples of gene evolution". New Scientist.
  4. ISSN 0002-1369
    .
  5. PMID 923591
    .
  6. PMID 7400094
    .
  7. ^ "GTDB - GCF_002049485.1". Genome Taxonomy Database, revision 95. 2020.
  8. PMID 1459943
    .
  9. ^
    PMID 17512009
    .
  10. ^ Negoro S, Taniguchi T, Kanaoka M, Kimura H, Okada H (July 1983). "Plasmid-determined enzymatic degradation of nylon oligomers". J. Bacteriol. 155 (1): 22–31.
    PMID 6305910
    .
  11. PMID 19889645
    .
  12. S2CID 4364682
    .
  13. ^
    PMID 29950566
    .
  14. ^ "Q57326". InterPro.
  15. ^ "MEROPS - the Peptidase Database".
  16. PMID 6585807
    .
  17. PMID 6585807
    .
  18. ^ Thwaites WM (Summer 1985). "New Proteins Without God's Help". Creation Evolution Journal. 5 (2): 1–3.
  19. ^ "Evolution and Information: The Nylon Bug". New Mexicans for Science Education. Retrieved 2023-09-27.
  20. ^ Than, Ker (2005-09-23). "Why scientists dismiss 'intelligent design'". NBC News. Retrieved 2023-09-27.
  21. ^ Miller, Kenneth R. Only a Theory: Evolution and the Battle for America's Soul (2008) pp. 80-82
  22. PMID 7646041
    .

External links
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