Organ bath
An organ chamber, organ bath, or isolated tissue bath, is a chamber in which isolated organs or tissues can be administered with
lactated Ringer's solution.[citation needed] Historically, they have also been called gut baths.[1]
Overview
It is used in
rats
.
For studying the effects of
high throughput screening, ultrahigh throughput screening and high content screening, pharmacogenomics
,
proteomics, and array technology have largely superseded the use of organ baths.[9] These techniques can allow more receptor specificity than organ bath preparations, as a single tissue sample can express many different receptor types.[citation needed]
The use of organ bath preparations for the measurement of physiological tissue responses to drug concentrations allows the generation of
Hill coefficient.[citation needed
]
Historical contributions
Examples of important contributions made using this technique include:
- The 1921 discovery by Otto Loewi of Vagusstoff using frog hearts resulted in the identification of acetylcholine as the first neurotransmitter.[10]
- Enkephalin using bioassays such as the mouse vas deferens as a bioassay.[11]
- Nitric oxide using both assays of bull retractor penis and aortic ring.[12]