Peripheral blood mononuclear cell
A peripheral blood mononuclear cell (PBMC) is any
These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation,[3] which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. The polymorphonuclear cells can be further isolated by lysing the red blood cells. Basophils are sometimes found in both the denser and the PBMC fractions.[3]
Clinical significance
Infections
Recent studies indicate that PBMCs may be susceptible to pathogenic infections,
Research uses
Many scientists conducting research in the fields of
PBMCs have been thought to be an important route of vaccination. PBMCs from cancer patients can be extracted and cultured in vitro. Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. Inflammatory cytokines are usually added to aid in antigen uptake and recognition by PBMCs.
References
- ISBN 978-1-4051-3603-7
- )
- ^ a b Miyahira, Andrea (22 Nov 2012). "Types of immune cells present in human PBMC". sanguinebio.com. Archived from the original on 22 July 2016. Retrieved 23 Sep 2014.
- PMID 30078192.
- ^ S2CID 53106591.
- ^ PMID 31549405.
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