Pantothenate kinase

Pantothenate kinase (

CoA is a necessary cofactor in all living organisms. It acts as the major acyl group carrier in many important cellular processes, such as the citric acid cycle (tricarboxylic acid cycle) and fatty acid metabolism. Consequently, pantothenate kinase is a key regulatory enzyme in the CoA biosynthetic pathway.^[3]

Types

Three distinct types of PanK has been identified - PanK-I (found in bacteria), PanK-II (mainly found in eukaryotes, but also in the Staphylococci) and PanK-III, also known as CoaX (found in bacteria). Eukaryotic PanK-II enzymes often occur as different isoforms, such as PanK1, PanK2, PanK3 and PanK4. In humans, multiple PanK isoforms are expressed by four genes. PANK1 gene encodes the PanK1α and PanK1β forms, and PANK2 and PANK3 encode PanK2 and PanK3, respectively.^[4] The four major isoforms found in mammals have different subcellular localizations. PanK1α is nuclear, while PanK1β and PanK3 are cytosolic. In mice, PanK2 is also cytosolic, while in humans, this enzyme is mitochondrial and nuclear.^[5] The tissue distribution of these isoforms also varies. In mouse models, PanK1 is the predominant species in the heart, liver and brown adipose tissue, along with the kidneys. PanK2 and PanK3 are more prominent in the brain and skeletal muscle, and PanK3 is particularly high in the intestines and white adipose tissue.^[6]

Structure

PanK-II

PanK-II contains two

The two ATP binding sites display cooperative behavior. The dimerization interface consists of two long helices, one from each monomer, that interact with each other. The C-terminal ends of the helices are held together by van der Waals interactions between valine and methionine residues of each monomer. The middle of the helices is attached by hydrogen bonds between asparagine residues. At the N-terminal end, each helix widens and forms a four-helix bundle with two shorter helices. This bundle consists of a hydrophobic core formed by non-polar residues that utilize van der Waals forces to further stabilize the dimer.^[4]

In the active site, pantothenate is oriented by hydrogen bonds between pantothenate and the side chains of aspartate, tyrosine, histidine, tyrosine, and asparagine residues.^[8] Asparagine, histidine, and arginine residues are involved in catalysis.

Human PanK-II isoforms PanK1α, PanK1β, PanK2, and PanK3 have a common, highly homologous catalytic core of approximately 355 residues.^[4] PanK1α and PanK1β are both encoded by the PANK1 gene and have the same catalytic domain of 363 amino acids, encoded by exons 2 through 7. The PanK1α transcript starts with exon 1α that encodes a 184-residue regulatory domain at the N-terminus. This region allows for feedback inhibition by free CoA and acyl-CoA and regulation by acetyl-CoA and malonyl-CoA. On the other hand, the PanK1β transcript starts with exon 1β, which produces a 10-residue N-terminus that does not include a feedback regulatory domain.^[9]

PanK-III

PanK-III also contains two protein domains, and the key catalytic residues of PanK-II are conserved. The monomer units of PanK-II and PanK-III are virtually identical, but they have distinctly different dimer assemblies. A study between the structures of Staphylococcus aureus type II and the Pseudomonas aeruginosa type III demonstrate that the PanK-II monomer has a loop region that is absent from the PanK-III monomer, and the PanK-III monomer has a loop region that is absent from the PanK-II monomer.^[10] This minor variation has a crucial difference on the dimerization interface in which the helices of the PanK-II dimer coil around one another and the helices of the PanK-III dimer interact at a 70° angle (Figure 2).^[11]

As a result of this difference in dimerization interface between PanK-II and PanK-III, the conformations of the substrate binding sites for ATP and pantothenate are also distinct.^[12][13]

Catalytic Mechanism

PanK-II

A proposed mechanism of the phosphoryl transfer reaction of PanK-II is a concerted mechanism with a dissociative transition state.

First, the

The regulation of pantothenate kinase is essential to controlling the intracellular CoA concentration.^[17] Pantothenate kinase is regulated via feedback inhibition by CoA and its thioesters (i.e., acetyl-CoA, malonyl-CoA).^[18] Inhibition of the human isoforms of PanK by acetyl-CoA varies dramatically. PanK1β is inhibited the least strongly, with an IC50 value of around 5 μM, while PanK2 is the most strongly inhibited, with an IC50 of around 0.1 μM.^[6]

CoA inhibits PanK activity by competitively binding to the ATP binding site and preventing ATP binding to Lys-101.^[14][15] Although CoA binds at the same site as ATP, they bind in distinct orientations, and their adenine moieties interact with the enzyme with nonoverlapping sets of residues. His-177, Phe-247, and Arg-106 are necessary for CoA recognition but not for ATP, and while Asn-43 and His-307 interact with the adenine base of ATP, His-177 and Phe-247 interact with the adenine base of CoA.^[16] Both molecules use Lys-101 to neutralize the charge on their respective phosphodiesters.

Nonesterified

Because PanK2 is so strongly inhibited by acetyl-CoA, an abundant metabolite in the mitochondria, this enzyme likely would not be active under physiological conditions without activators.^[6] Palmitoyl-carnitine and other long-chain acyl-carnitines can reverse acetyl-CoA inhibition and can activate PanK2 without acetyl-CoA present. Palmitoyl-carnitine is competitive with acetyl-CoA.^[20] The activation of PanK2 by palmitoyl-carnitine and other long-chain acyl-carnitines sheds light on the regulatory pathways of this enzyme: Under normal conditions, PanK2 is likely inhibited by high levels of acetyl-CoA. Without CoA production, fatty acid oxidation decreases, leading to an increase in long-chain acyl-carnitines.^[19] These acyl-carnitines can then reduce inhibition by acetyl-CoA, activating PanK2 and increasing CoA biosynthesis. PanK3 is also activated by palmitoyl-carnitine and other long-chain acyl-carnitines, including oleoyl-carnitine.^[21]

PanK-III

The regulation outlined above corresponds to PanK-II. PanK-III is resistant to feedback inhibition.^[10][12][13]

Genes

In humans:

• PANK2, PANK3, PANK4
  .

The PANK2 gene encodes for PanK2, which regulates the formation of CoA in mitochondria, the cell’s energy-producing centers.^[22] PANK2 mutation is the cause of Pantothenate kinase-associated neurodegeneration (PKAN), formerly called Hallervorden-Spatz syndrome. This rare disease presents with profound dystonia, spasticity and is often fatal.

There are many mutations in PanK2 that lead to PKAN. In a survey of several common mutations, it was found that several of these mutations did not cause a major loss in the catalytic activity of PanK2, indicating that loss of catalytic function of this enzyme is not fully responsible for this disease.^[23]

References