Petri dish
A Petri dish (alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to hold
What was later called Petri dish was originally developed by German physician Robert Koch in his private laboratory in 1881, as a precursor method. Petri, as assistant to Koch, at Berlin University made the final modifications in 1887 as used today.
Features and variants
This section
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Petri dishes are usually
Petri dishes were traditionally reusable and made of glass; often of heat-resistant borosilicate glass for proper sterilization at 120–160 °C.[9]
Since the 1960s, plastic dishes, usually disposable, are also common.[14]
The dishes are often covered with a shallow transparent lid, resembling a slightly wider version of the dish itself. The lids of glass dishes are usually loose-fitting.[9] Plastic dishes may have close-fitting covers that delay the drying of the contents.[15] Alternatively, some glass or plastic versions may have small holes around the rim, or ribs on the underside of the cover, to allow for air flow over the culture and prevent water condensation.[16]
Some Petri dishes, especially plastic ones, usually feature rings and/or slots on their lids and bases so that they are less prone to sliding off one another when stacked or sticking to a smooth surface by suction.[15]
Small dishes may have a protruding base that can be secured on a microscope stage for direct examination.[17]
Some versions may have grids printed on the bottom to help in measuring the density of cultures.[18][12][13]
A microplate is a single container with an array of flat-bottomed cavities, each being essentially a small Petri dish. It makes it possible to inoculate and grow dozens or hundreds of independent cultures of dozens of samples at the same time. Besides being much cheaper and convenient than separate dishes, the microplate is also more amenable to automated handling and inspection.
History
The Petri dish was developed by German physician
Koch publicly demonstrated his plating method at the Seventh
Petri made changes in how the circular dish was used. It is often asserted that Petri developed a new culture plate,[28][29][30] but this is incorrect. Instead of using a separate glass slide or plate on which culture media were placed, Petri directly placed media into the glass dish, eliminating unnecessary steps such as transferring the culture media, using the wet paper, and reducing the chance of contamination.[19] He published the improved method in 1887 as "Eine kleine Modification des Koch’schen Plattenverfahrens" ("A minor modification of the plating technique of Koch").[6] Although it could have been named "Koch dish",[24] the final method was given an eponymous name Petri dish.[31]
Uses
Microbiology
Petri dishes are widely used in biology to
The
The dishes are then left undisturbed for hours or days while the organism grows, possibly in an incubator. They are usually covered, or placed upside-down, to lessen the risk of contamination from airborne spores.
While Petri dishes are widespread in microbiological research, smaller dishes tend to be used for large-scale studies in which growing cells in Petri dishes can be relatively expensive and labor-intensive.[32][33]
Contamination detection and mapping
Petri dishes can be used to visualize the location of contamination on surfaces, such as kitchen counters and utensils,[34] clothing, food preparation equipment, or animal and human skin.[35][36]
For this application, the Petri dishes may be filled so that the culture medium protrudes slightly above the edges of the dish to make it easier to take samples on hard objects. Shallow Petri dishes prepared in this way are called Replicate Organism Detection And Counting (RODAC) plates and are available commercially.[37][38]
Cell culture
Petri dishes are also used for cell cultivation of isolated cells from eukaryotic organisms, such as in immunodiffusion studies, on solid agar or in a liquid medium.
Botany and agriculture
Petri dishes may be used to observe the early stages of plant germination, and to grow plants asexually from isolated cells.
Entomology
Petri dishes may be convenient enclosures to study the behavior of insects and other small animals.
Chemistry
Due to their large open surface, Petri dishes are effective containers to evaporate
Sample storage and display
Petri dishes also make convenient temporary storage for samples, especially liquid, granular, or powdered ones, and small objects such as insects or seeds. Their transparency and flat profile allows the contents to be inspected with the naked eye, magnifying glass, or low-power microscope without removing the lid.
In popular culture
The Petri dish is one of a small number of laboratory equipment items whose name entered popular culture. It is often used metaphorically, e.g. for a contained community that is being studied as if they were microorganisms in a biology experiment, or an environment where original ideas and enterprises may flourish.[7][8][39]
See also
References
- ISBN 978-8121924177
- ^ Mosby's Dental Dictionary (2nd ed.). Elsevier. 2008. Retrieved 2010-02-11.
- doi:10.1111/j.1438-8677.1998.tb00670.x. Archived from the original(PDF) on 2015-09-24. Retrieved 2015-07-19.
- American Heritage Dictionary.
- ^ Petri, R.J. (1887). "Eine kleine Modification des Koch'schen Plattenverfahrens" [A small modification of Koch's plate method]. Centralblatt für Bakteriologie und Parasitenkunde (in German). 1: 279–80.
- ^ a b Petri, R.J. (1887). "Eine kleine Modification des Koch'schen Plattenverfahrens" [A small modification of Koch's plate method]. Centralblatt für Bakteriologie und Parasitenkunde (English Translation, Braus, 2020) (in German). 1: 279–80.
- ^ ISBN 978-1510732346
- ^ a b Isabel Slone (2018): "What Does the Mall Goth Nostalgia Trend Really Mean?". Quote: "mall goth" was a style of dress that combined the hallmarks of punk, goth and metal subcultures and thrived like bacteria in the petri dish of the early 2000s. Online article in the Fashion Magazine website, May 22, 2018. Accessed on 2019-10-25.
- ^ a b c (2019): "Product 4909050: PYREX reusable Petri dishes: complete". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ (2019): "Product BRB011: Petri Dish 200 mm, borosilicate". Rogo-Sampaic online catalog. Accessed on 2019-10-25.
- ^ (2019): "Product BTX9302 Corning 100 x 25mm bio-agricultural Petri dishes". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ a b (2019): "Item 1219C98: Square Petri dish w/ grid". Thomas Scientific online catalog. Accessed on 2019-10-25.
- ^ a b (2019): "Product 11708573: Gosselin Square Petri Dish". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ (2019): "Product BP94S01: Corning 100 x 15mm Polystyrene Petri Dishes". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ a b (2019): "Item 09-720-500: Fisherbrand disposable Petri dishes". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ (2019): "Item SB93102: Corning 100x15mm Petri dish with three vents". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ (2019): "Product PD1504700 MilliporeSigma PetriSlide for contamination analysis". Fischer Scientific online catalog. Accessed on 2019-10-25.
- ^ (2019): "Item 41044: Petri dishes made of glass with grid and cover". Assistent (Karl Hecht) online catalog. Accessed on 2019-10-25
- ^ ISSN 0007-5027.
- ^ Kassinger, Ruth (2019). Slime How Algae Created Us, Plague Us, and Just Might Save Us. Boston, New York: Houghton Mifflin Harcourt. p. 124.
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- ^ Kasia Galazka (2015): "Here's A Gorgeous Petri Dish Handprint Of An 8-Year-Old After He Played Outside". BuzzFeed.News online article, June 9, 2015. Accessed on 2019-10-25.
- ^ Sonja Bäumel (2009): "Oversized petri dish". Culture of microorganisms from the artist's skin pressed onto a body-size culture plate, photographed over the span of 44 days. Part of her (In)visible membrane project. Wageningen, Germany. Accessed on 2019-10-25.
- ISBN 978-1420014853
- ^ "Definition of petri dish".
- ^ (2019): "Emoji List, v12.1". Webpage at the Unicode Consortium website. Accessed on 2019-10-25.