Phosphorylase

Phosphorylase
ExPASy		NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
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In

) to an acceptor.

A-B + P ⇌ A + P-B

They include

.

Phosphorylase is also a common name used for glycogen phosphorylase in honor of Earl W. Sutherland Jr., who in the late 1930s discovered it as the first phosphorylase.^[1]

Function

Phosphorylases should not be confused with

, which remove phosphate groups. In more general terms, phosphorylases are enzymes that catalyze the addition of a phosphate group from an ). A phosphatase removes a phosphate group from a donor using water, whereas a kinase transfers a phosphate group from a donor (usually ATP) to an acceptor.

Enzyme name	Enzymes class	Reaction	Notes
Phosphorylase	Transferase (EC 2.4 and EC 2.7.7)	A-B + H-OP ⇌ A-OP + H-B	transfer group = A = nucleotidyl - group
Phosphatase	Hydrolase (EC 3)	P-B + H-OH ⇌ P-OH + H-B
Kinase	Transferase (EC 2.7.1-2.7.4)	P-B + H-A ⇌ P-A + H-B	transfer group = P
P = phosphonate group, OP = phosphate group, H-OP or P-OH = inorganic phosphate

Types

The phosphorylases fall into the following categories:

• Glycosyltransferases (EC 2.4)
  • Enzymes that break down glucans by removing a glucose residue (break O-glycosidic bond)
    • glycogen phosphorylase
    • starch phosphorylase
    • maltodextrin phosphorylase
  • Enzymes that break down nucleosides into their constituent bases and sugars (break N-glycosidic bond)
    • Purine nucleoside phosphorylase (PNPase)
• Nucleotidyltransferases (EC 2.7.7)
  • Enzymes that have phosphorolytic 3' to 5' exoribonuclease activity (break phosphodiester bond)
    • RNase PH
    • Polynucleotide Phosphorylase
      (PNPase)

All known phosphorylases share catalytic and structural properties.^[2]

Activation

Phosphorylase a is the more active R form of glycogen phosphorylase that is derived from the phosphorylation of the less active R form, phosphorylase b with associated AMP. The inactive T form is either phosphorylated by phosphoylase kinase and inhibited by glucose, or dephosphorylated by phosphoprotein phosphatase with inhibition by ATP and/or glucose 6-phosphate. Phosphorylation requires ATP but dephosphorylation releases free inorganic phosphate ions.

Pathology

Some disorders are related to phosphorylases:

• Glycogen storage disease type V - muscle glycogen
• Glycogen storage disease type VI - liver glycogen

See also

• Hydrolase

References

External links

• Muscle phosphorylase deficiency - McArdle's Disease Website
• Phosphorylases at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

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