Protease

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Ribbon diagram of a protease (TEV protease) complexed with its peptide substrate in black with catalytic residues in red.(PDB: 1LVB​)

A protease (also called a peptidase, proteinase, or proteolytic enzyme)

peptide bonds within proteins by hydrolysis, a reaction where water breaks bonds. Proteases are involved in numerous biological pathways, including digestion of ingested proteins, protein catabolism (breakdown of old proteins),[3][4] and cell signaling
.

In the absence of functional accelerants, proteolysis would be very slow, taking hundreds of

catalytic mechanisms
.

Classification

Based on catalytic residue

Proteases can be classified into seven broad groups:[6]

Proteases were first grouped into 84 families according to their evolutionary relationship in 1993, and classified under four catalytic types:

carbonyl group. One way to make a nucleophile is by a catalytic triad, where a histidine residue is used to activate serine, cysteine, or threonine as a nucleophile. This is not an evolutionary grouping, however, as the nucleophile types have evolved convergently in different superfamilies, and some superfamilies show divergent evolution to multiple different nucleophiles. Metalloproteases, aspartic, and glutamic proteases utilize their active site residues to activate a water molecule, which then attacks the scissile bond.[8]

Peptide lyases

A seventh catalytic type of proteolytic enzymes, asparagine peptide lyase, was described in 2011. Its proteolytic mechanism is unusual since, rather than hydrolysis, it performs an elimination reaction.[9] During this reaction, the catalytic asparagine forms a cyclic chemical structure that cleaves itself at asparagine residues in proteins under the right conditions. Given its fundamentally different mechanism, its inclusion as a peptidase may be debatable.[9]

Based on evolutionary phylogeny

An up-to-date classification of protease evolutionary

PA clan where P indicates a mixture of nucleophile families). Within each 'clan', proteases are classified into families based on sequence similarity (e.g. the S1 and C3 families within the PA clan). Each family may contain many hundreds of related proteases (e.g. trypsin, elastase, thrombin and streptogrisin
within the S1 family).

Currently more than 50 clans are known, each indicating an independent evolutionary origin of proteolysis.[10]

Based on optimal pH

Alternatively, proteases may be classified by the optimal pH in which they are active:

  • Acid proteases
  • Neutral proteases involved in
    calpains
    .
  • Basic proteases
     (or alkaline proteases)

Enzymatic function and mechanism

substrate protein in red and water in blue. The top panel shows 1-step hydrolysis where the enzyme uses an acid to polarise water, which then hydrolyses the substrate. The bottom panel shows 2-step hydrolysis where a residue within the enzyme is activated to act as a nucleophile
(Nu) and attack the substrate. This forms an intermediate where the enzyme is covalently linked to the N-terminal half of the substrate. In a second step, water is activated to hydrolyse this intermediate and complete catalysis. Other enzyme residues (not shown) donate and accept hydrogens and electrostatically stabilise charge build-up along the reaction mechanism.
See also: Catalytic triad

Proteases are involved in

endopeptidases, such as trypsin, chymotrypsin, pepsin, papain, elastase
).

Catalysis

Catalysis is achieved by one of two mechanisms:

  • Aspartic, glutamic, and metallo-proteases activate a water molecule, which performs a nucleophilic attack on the peptide bond to hydrolyze it.
  • Serine, threonine, and cysteine proteases use a nucleophilic residue (usually in a
    covalently
    link the protease to the substrate protein, releasing the first half of the product. This covalent acyl-enzyme intermediate is then hydrolyzed by activated water to complete catalysis by releasing the second half of the product and regenerating the free enzyme

Specificity

Proteolysis can be highly promiscuous such that a wide range of protein substrates are hydrolyzed. This is the case for digestive enzymes such as trypsin, which have to be able to cleave the array of proteins ingested into smaller peptide fragments. Promiscuous proteases typically bind to a single amino acid on the substrate and so only have specificity for that residue. For example, trypsin is specific for the sequences ...K\... or ...R\... ('\'=cleavage site).[12]

Conversely some proteases are highly specific and only cleave substrates with a certain sequence. Blood clotting (such as thrombin) and viral polyprotein processing (such as TEV protease) requires this level of specificity in order to achieve precise cleavage events. This is achieved by proteases having a long binding cleft or tunnel with several pockets that bind to specified residues. For example, TEV protease is specific for the sequence ...ENLYFQ\S... ('\'=cleavage site).[13]

Degradation and autolysis

Proteases, being themselves proteins, are cleaved by other protease molecules, sometimes of the same variety. This acts as a method of regulation of protease activity. Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen).

Biodiversity of proteases

Proteases occur in all organisms, from

viruses
. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades (e.g., the blood-clotting cascade, the complement system, apoptosis pathways, and the invertebrate prophenoloxidase-activating cascade). Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or completely break down a peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function or digesting it to its principal components), it can be an activation of a function, or it can be a signal in a signalling pathway.

Plants

Plant genomes encode hundreds of proteases, largely of unknown function. Those with known function are largely involved in

developmental regulation.[14] Plant proteases also play a role in regulation of photosynthesis.[15]

Animals

Proteases are used throughout an organism for various metabolic processes. Acid proteases secreted into the stomach (such as

haemotoxin
and interfere with the victim's blood clotting cascade. Proteases determine the lifetime of other proteins playing important physiological roles like hormones, antibodies, or other enzymes. This is one of the fastest "switching on" and "switching off" regulatory mechanisms in the physiology of an organism.

By a complex cooperative action, proteases can catalyze cascade reactions, which result in rapid and efficient amplification of an organism's response to a physiological signal.

Bacteria

hydrolyse the peptide bonds in proteins and therefore break the proteins down into their constituent amino acids. Bacterial and fungal proteases are particularly important to the global carbon and nitrogen cycles in the recycling of proteins, and such activity tends to be regulated by nutritional signals in these organisms.[16] The net impact of nutritional regulation of protease activity among the thousands of species present in soil can be observed at the overall microbial community level as proteins are broken down in response to carbon, nitrogen, or sulfur limitation.[17]

Bacteria contain proteases responsible for general protein quality control (e.g. the AAA+

unfolded or misfolded proteins
.

A secreted bacterial protease may also act as an exotoxin, and be an example of a virulence factor in bacterial pathogenesis (for example, exfoliative toxin). Bacterial exotoxic proteases destroy extracellular structures.

Viruses

The genomes of some

polyprotein, which needs a protease to cleave this into functional units (e.g. the hepatitis C virus and the picornaviruses).[18] These proteases (e.g. TEV protease) have high specificity and only cleave a very restricted set of substrate sequences. They are therefore a common target for protease inhibitors.[19][20]

Archaea

Archaea use proteases to regulate various cellular processes from cell-signaling, metabolism, secretion and protein quality control.[21][22] Only two ATP-dependent proteases are found in archaea: the membrane associated LonB protease and a soluble 20S proteosome complex .[21]

Uses

Main article: Proteases (medical and related uses)

The field of protease research is enormous. Since 2004, approximately 8000

papers related to this field were published each year.[23] Proteases are used in industry, medicine and as a basic biological research tool.[24][25]

Digestive proteases are part of many

affinity tags
in a controlled fashion. Protease-containing plant-solutions called vegetarian rennet have been in use for hundreds of years in Europe and the Middle East for making kosher and halal Cheeses. Vegetarian rennet from Withania coagulans has been in use for thousands of years as a Ayurvedic remedy for digestion and diabetes in the Indian subcontinent. It is also used to make Paneer.

Inhibitors

Main articles: Protease inhibitor (biology) and Protease inhibitor (pharmacology)

The activity of proteases is inhibited by

plasminogen activator inhibitor-1 (which protects the body from inadequate coagulation by blocking protease-triggered fibrinolysis), and neuroserpin.[27]

Natural protease inhibitors include the family of

viruses, with HIV/AIDS among them, depend on proteases in their reproductive cycle. Thus, protease inhibitors are developed as antiviral
therapeutic agents.

Other natural protease inhibitors are used as defense mechanisms. Common examples are the trypsin inhibitors found in the seeds of some plants, most notable for humans being soybeans, a major food crop, where they act to discourage predators. Raw soybeans are toxic to many animals, including humans, until the protease inhibitors they contain have been denatured.

See also

References

  1. ^ "Proteolytic enzyme | Description, Types, & Functions | Britannica".
  2. PMID 18650443
    .
  3. ^
    PMID 24931469
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  4. ^
    PMID 17051221
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  5. doi:10.1021/ja954077c
    . To assess the relative proficiencies of enzymes that catalyze the hydrolysis of internal and C-terminal peptide bonds [...]
  6. PMID 22016395
    .
  7. PMID 8439290
    .
  8. ^ Sanman, Laura E. (June 2014). "Activity-Based Profiling of Proteases". Annual Review of Biochemistry. 83: 249–273.
  9. ^
    PMID 21832066
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  10. ^
    PMID 19892822
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  11. ISBN 978-1-4160-2973-1
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  14. PMID 18257708
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  15. PMID 15535125
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  16. doi:10.1016/j.soilbio.2006.01.006. Archived from the original
    on 2021-04-28. Retrieved 2018-12-29.
  17. ^ Sims GK, Wander MM (2002). "Proteolytic activity under nitrogen or sulfur limitation". Appl. Soil Ecol. 568: 1–5.
  18. PMID 12475203
    .
  19. PMID 23016690
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  20. PMID 27090931
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  21. ^
    PMID 25774151
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  22. PMID 32953999
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  23. ISBN 978-0-12-079610-6
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  24. ISBN 978-1-85578-147-4
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  25. S2CID 84748291
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  26. PMID 11427378
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  27. PMID 15060002
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External links

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Protease
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