Respiratory burst

Source: Wikipedia, the free encyclopedia.

Respiratory burst (or oxidative burst) is the rapid release of the reactive oxygen species (ROS), superoxide anion (O−
2) and hydrogen peroxide (H
2O
2), from different cell types.

This is usually utilised for mammalian

fertilization. It may also occur in plant cells
.

Immunity

Immune cells can be divided into myeloid cells and lymphoid cells. Myeloid cells, including macrophages and neutrophils, are especially implicated in the respiratory burst.[1] They are phagocytic, and the respiratory burst is vital for the subsequent degradation of internalised bacteria or other pathogens. This is an important aspect of the innate immunity
.

Respiratory burst requires a 10 to 20 fold increase in oxygen consumption through NADPH oxidase (NOX2 in humans) activity. NADPH is the key substrate of NOX2, and bears reducing power. Glycogen breakdown is vital to produce NADPH. This occurs via the pentose phosphate pathway.

The NOX2 enzyme is bound in the phagolysosome membrane. Post bacterial phagocytosis, it is activated, producing superoxide via its redox centre, which transfers electrons from cytosolic NADPH to O2 in the phagosome.[2]


2O2 + NADPH —> 2O2•– + NADP+ + H+


The superoxide can then

free radicals
.


Pathways for reactive species generation

Generation of reactive oxygen and reactive nitrogen species in the phagolysosome, implicated in respiratory burst.

There are 3 main pathways for the generation of reactive oxygen species or reactive nitrogen species (RNS) in effector cells:[3]

  1. Superoxide dismutase (or alternatively, myeloperoxidase) generates hydrogen peroxide from superoxide. Hydroxyl radicals are then generated via the Haber–Weiss reaction or the Fenton reaction, of which are both catalyzed by Fe2+.

    O2•–+ H2O2 —> •OH + OH– + O2

  2. In the presence of halide ions, prominently chloride ions, myeloperoxidase uses hydrogen peroxide to produce hypochlorous acid.

    H2O2 + Cl− —> ClO− + H2O

  3. Nitric oxide synthase (the inducible isoform, iNOS, in immunity) catalyses the production of nitric oxide from L-arginine.

    2L-arginine + 3NADPH + 3 H+ + 4O2 —> 2citrulline + 2NO• + 4H2O + 3NADP+

Nitric oxide may react with superoxide anions to produce peroxynitrite anion.

O2•− + NO• → ONO2−


Defense against pathogens

The exposure to these reactive species in the respiratory burst results in pathology. This is due to oxidative damage to the engulfed bacteria.

Notably, peroxynitrite is a very strong

FeS, releasing Fe2+ for the Fenton reaction. Peroxynitrite may also react with various amino acids in the peptide chain, thereby altering protein structure and subsequently, protein function. It most commonly oxidises cysteine, and may indirectly induce tyrosine nitration through other generated RNS. Altered protein function includes changes in enzyme catalytic activity, cytoskeletal organisation and cell signal transduction.[4]

Hypochlorous acid reacts with a range of biomolecules, including DNA, lipids and proteins. HClO may oxidise cysteines and

disulfide bonds, inducing protein crosslinking. Both oxidations result in protein aggregation, and ultimately, cell death.[5] Sulfhydryl groups can be oxidised up to three times by three HClO molecules, forming sulfenic acids, sulfinic acids and R–SO3H, which are increasingly irreversible and bactericidal.[6][7] Meanwhile, methionine oxidation is reversible. HOCl can also react with primary or secondary amines, producing chloroamines which are toxic to bacteria.[8][9]
Protein cross linking and aggregation may also occur, as well as disruption of FeS groups.

Integral to hypochlorous acid formation is myeloperoxidase. Myeloperoxidase is most abundant in neutrophils, wherein phagocytosis is accompanied by degranulation. This is the fusion of granules with the phagolysosome, releasing their contents, including myeloperoxidase.[10] As many microbicidal products are formed during respiratory burst, the importance of individual molecules in killing invading pathogens is not wholly understood.

Due to the high toxicity of generated antimicrobial products including ROS, neutrophils have a short life span to limit host tissue damage during inflammation.

Disease

Chronic Granulomatous Disease is an inherited disease of human neutrophils, wherein NOX2 is defective. Phagocytosis may still occur, but without proper functioning NOX2, there is no superoxide production, and therefore no respiratory burst. The bacterial infection is not cleared.[11]

Cellular signalling

Non-phagocytic cells

In non-phagocytic cells, oxidative burst products are used in intracellular signalling pathways. The generated ROS achieve this via shifting the cell

glutathione disulphide (GSH:GSSG).[12] Antioxidant enzymes counterbalance redox signalling by eliminating the involved molecules, importantly superoxide anion and nitric oxide. Redox signalling is critical for normal processes such as proliferation, differentiation, as well as vascular function and neurotransmission. It is also involved in disease states such as cancer
.

The NADPH oxidase isoform NOX1 transiently produces a burst of superoxide in response to growth factor (e.g. EGF) stimulation of respective receptors.[13] Superoxide is dismutated to hydrogen peroxide at a rate close to the diffusion-limited rate. This spatial restriction for superoxide‘s dismutation allows for specificity of redox signalling. Specificity is also ensured by NOX1 localisation in specific microdomains in the cell’s plasma membrane. Through channels such as aquaporin or diffusion, hydrogen peroxide enters the cytosol. There, it oxidises the cysteine groups of redox-sensitive proteins, which can then transduce signals.[14]

Macrophages

Oxidative burst in phagocytes is most commonly associated with bacterial killing. However, macrophages, especially alveolar macrophages, usually produce far lower levels of ROS than neutrophils, and may require activation for their bactericidal properties. Instead, their transient oxidative burst regulates the inflammatory response by inducing cytokine synthesis for redox signalling, resulting in an influx of neutrophils and activated macrophages.[15]

Cancer cells

Cancer cells can manipulate cell signalling by producing excess levels of ROS, thereby constitutively activating pathways to promote their cellular growth and proliferation.

oncogenic KRAS cells. However, in oncogenic Kras mice fibroblasts, NADPH oxidase inhibitors have been shown to be sufficient to block these growth factor pathways.[17] Tumorigenic cells also simultaneously maintain high levels of antioxidants to protect against cancer cell death.[18]

Fertilisation

Most notably, oxidative burst post fertilisation can be seen in the sea urchin egg. This is believed to be evolutionally divergent from that in neutrophils.

Hydrogen peroxide is produced by egg oxidase activity following an increase in oxygen consumption.[19] This is essential for the cross-linking of the ovum proteins to prevent lethal polyspermy. Hydrogen peroxide itself is also spermicidal. However, the generated reactive species are maintained at lower levels than in immunity to protect the fertilised egg itself from oxidative damage. This is achieved by the elimination of hydrogen peroxide primarily through the dual function of the same egg oxidase, and secondarily through cytoplasmic ROS scavengers, such as catalase and glutathione.[20]

In plants

Oxidative burst acts as a defence mechanism to pathogen infection in plants. This is seen post

plant immunity, the NADPH oxidase subunits RbohD and RbohF have overlapping functions are expressed in different tissues and at different levels.[24][25]
However, in contrast to animal phagocytes, wherein generated ROS are contained in the sealed phagolysosome, oxidative burst in plants is not contained. Consequently, generated ROS bear additional effects alongside pathogen toxicity. Hydrogen peroxide induces oxidative cross-linking of the plant’s cell wall glycoproteins.[26][27] This reduces susceptibility to enzymatic degradation by pathogens.[28] Systemic acquired resistance, which is analogous to innate immunity in animals, is also induced in the exposed plant cells.[29] Hydrogen peroxide exposure may also result in hypersensitive response, which is the death of a small number of host cells at the site of infection, for the purpose of limiting pathogenic infection.[30][31] ROS production in plants can be used as a readout for successful pathogen recognition via a luminol-peroxidase based assay.[32]

References

  1. PMID 33669824
    .
  2. PMID 16987010
    .
  3. PMID 14527285
    .
  4. PMID 17237348
    .
  5. PMID 4745674
    .
  6. PMID 2986713
    .
  7. PMID 9439597
    .
  8. PMID 21896680
    .
  9. S2CID 45991444
    .
  10. PMID 10830774
    .
  11. ^ Fernandez J. "Chronic Granulomatous Disease (CGD) - Immunology; Allergic Disorders". MSD Manual Professional Edition. Retrieved 2020-03-12.
  12. PMID 11368918
    .
  13. PMID 12471082
    .
  14. PMID 30287799
    .
  15. PMID 12471082
    .
  16. PMID 1846317
    .
  17. S2CID 19733670
    .
  18. S2CID 20604657
    .
  19. doi:10.1515/bchm2.1908.57.1-2.1
    .
  20. PMID 15572124
    .
  21. doi:10.1016/0048-4059(85)90044-X
    .
  22. S2CID 12312001
    .
  23. PMID 9114072
    .
  24. PMID 11756663
    .
  25. PMID 26798024
    .
  26. S2CID 12312001
    .
  27. S2CID 23319754
    .
  28. PMID 12244231
    .
  29. PMID 8266079
    .
  30. PMID 11607542
    .
  31. S2CID 1488844
    .
  32. doi:10.1094/phyto-79-974
    .

External links
Retrieved from "https://en.wikipedia.org/w/index.php?title=Respiratory_burst&oldid=1183601334"