SYBR Green I
(Redirected from
SYBR Green
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IUPAC name
N',N'-dimethyl-N-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N-propylpropane-1,3-diamine
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Identifiers | |
3D model (
JSmol ) |
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ChEMBL | |
PubChem CID
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CompTox Dashboard (EPA)
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Properties | |
C32H37N4S+ | |
Molar mass | 509.73 g/mol |
Solubility | Normally supplied solvated in dimethylsulfoxide
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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SYBR Green I (SG) is an asymmetrical
Molecular Probes Inc., now owned by Thermo Fisher Scientific. SYBR Green I binds to DNA. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The stain preferentially binds to double-stranded DNA, but will stain single-stranded (ss) DNA with lower performance. SYBR Green can also stain RNA
with a lower performance than ssDNA.
Uses
SYBR Green finds usage in several areas of
RNase
treatment may be required to reduce background from RNA in the cells.
Safety
SYBR Green I is marketed as a replacement for ethidium bromide, a potential human mutagen, as both safer to work with and free from the complex waste disposal issues of ethidium bromide. However any small molecule capable of binding DNA with high affinity is a possible carcinogen, including SYBR Green.
In a study using the Ames test, which measures the ability of chemicals to cause mutations, when assayed at the same concentration SYBR Green I was on the order of 30 times less mutagenic than ethidium bromide.[3]
Similar cyanine dyes
- PicoGreen (PG)
- SYBR Safe
- SYBR Gold
- Thiazole orange (TO)
- Oxazole yellow (YO)
- Safe-Green
- Chai Green