Salting out

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For the abortion method, see Instillation abortion.

Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out)

Dialysis
can be used to remove the salt if needed.

Principle

Salt compounds dissociate in aqueous solutions. This property is exploited in the process of salting out. When the salt concentration is increased, some of the water molecules are attracted by the salt ions, which decreases the number of water molecules available to interact with the charged part of the protein.[3]

The principle of salting in and salting out technique, based on increasing salt concentration.

There are

hydrophilic amino acids in protein
molecules. After protein folding in aqueous solution, hydrophobic amino acids usually form protected hydrophobic areas while hydrophilic amino acids interact with the molecules of solvation and allow proteins to form hydrogen bonds with the surrounding water molecules. If enough of the protein surface is hydrophilic, the protein can be dissolved in water.[4]

When salt is added to the solution, there is more frequent interaction between solvent molecules and salt ions. As a result, the protein and salt ions compete to interact with the solvent molecules with the result that there are fewer solvent molecules available for interaction with the protein molecules than before. The

hydrophobic interactions with each other.[5] After dissociation in a given solvent, the negatively charged atoms from a chosen salt begin to compete for interactions with positively charged molecules present in the solution. Similarly, the positively charged cations compete for interactions with the negatively charged molecules of the solvent. This process is known as salting out.[citation needed
]

Soaps are easily precipitated by concentrated salt solution, the metal ion in the salt reacts with the fatty acids forming back the soap and glycerin (glycerol). To separate glycerin from the soap, the pasty boiling mass is treated with brine (NaCl solution). Contents of the kettle salt out (separate) into an upper layer that is a curdy mass of impure soap and a lower layer that consists of an aqueous salt solution with the glycerin dissolved in it. The slightly alkaline salt solution, termed spent lye, is extracted from the bottom of the pan or kettle and may be subsequently treated for glycerin recovery.[citation needed]

Application

As different proteins have different compositions of amino acids, different protein molecules precipitate at different concentrations of salt solution.[citation needed]

Unwanted proteins can be removed from a protein solution mixture by salting out as long as the solubility of the protein in various concentrations of salt solution is known. After removing the precipitate by filtration or centrifugation, the desired protein can be precipitated by altering the salt concentration to the level at which the desired protein becomes insoluble.[6]

One demerit of salting out in purification of proteins is that, in addition to precipitating a specific protein of interest, contaminants are also precipitated as well. Thus to obtain a purer protein of interest, additional purification methods such as ion exchange chromatography may be required.[7]

See also

References

  1. ^ Genck, Wayne (2010). "Make The Most of Antisolvent Crystallization". Chemical Processing. PutmanMedia.
  2. doi:10.2147/BSAM.S46573
    .
  3. PMID 6525340
    .
  4. PMID 31501317
    .
  5. ISBN 978-0-444-63688-1
    .
  6. PMID 18429073
    .
  7. PMID 24674064
    .

Further reading

External links
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