Signal recognition particle RNA
RN7SL1 | ||||||
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Identifiers | ||||||
Ensembl |
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UniProt |
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RefSeq (mRNA) |
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RefSeq (protein) |
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Location (UCSC) | Chr 14: 49.59 – 49.59 Mb | n/a | ||||
PubMed search | [2] | n/a |
View/Edit Human |
The signal recognition particle RNA, (also known as 7SL, 6S, ffs, or 4.5S RNA) is part of the
In this way SRP directs the movement of
The common
The
In most
In eukaryotes and archaea, eight helical elements fold into the
Discovery
SRP RNA was first detected in avian and murine
Transcription and processing
Eukaryotic SRP RNAs are transcribed from
Function
Co-translational translocation
The SRP RNA is an integral part of the small and the large domain of the SRP. The function of the small domain is to delay protein translation until the ribosome-bound SRP has an opportunity to associate with the membrane-resident
Post-translational transport
SRP participates also in the sorting of
Structure
In 2005, a nomenclature for all SRP RNAs proposed a numbering system of 12 helices. Helix sections are named with a lower case letter suffix (e.g. 5a). Insertions, or helix "branches" are given dotted numbers (e.g. 9.1 and 12.1).
The SRP RNA spans a wide
Motifs
Four conserved features (motifs) have been identified (shown in the Figure in dark gray): the (1) SRP54 binding motif, (2) Helix 6 GNAR tetraloop motif, (3) 5e motif, and (4) UGU(NR) motif.[citation needed]
SRP54 binding
The asymmetric loop between helical sections 8a and 8b and the adjacent base paired 8b section are a prominent property of every SRP RNA. Helical section 8b contains non-
Helix 6 GNAR tetraloop
The SRP RNAs of
5e
The 11 nucleotides of the 5e motif form four
UGU(NR)
The UGU(NR) motif connects helices 3 and 4 in the small (Alu) SRP domain.
Secondary
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Bacterial SRP RNA (4.5S RNA) from E. coli
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Bacterial SRP RNA (6S RNA) from Bacillus subtilis
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Archaeal SRP RNA Archaeoglobus fulgidus
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Eukaryotic protist SRP RNA from Trypanosoma brucei
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Eukaryotic yeast SRP RNA from Saccharomyces cerevisiae
Tertiary
SRP RNA | |
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Identifiers | |
Rfam | CL00003 |
Other data | |
PDB structures | PDBe 2IY3, 1Z43, 1RY1, 1QZW, 1MFQ, 1L9A, 1LNG,1JID, 1E8S, 1E8O, 1DUL, 1DUH,1D4R, 28SR, 28SP |
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SRP19-7S.S SRP RNA complex from M. jannaschii[27]
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S-domain of human SRP[30]
Binding proteins
One or more SRP
SRP9 and SRP14
SRP9 and SRP14 are
SRP19
SRP19 is found in the SRP of
SRP54
Protein SRP54 (named Ffh in the bacteria) is an essential component of every SRP. It is composed of three functional domains: the N-terminal (N) domain, the GTPase (G) domain, and the methionine-rich (M) domain.[36][37]
SRP68 and SRP72
Proteins SRP68 and SRP72 are structurally unrelated constituents of the large domain of the
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000276168 - Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ S2CID 4328237.
- ^ PMID 19838050.
- ^ PMID 12140321.
- ^ PMID 11839493.
- ^ PMID 10678824.
- PMID 11243816.
- PMID 4321311.
- PMID 4530311.
- S2CID 26928799.
- PMID 7309795.
- S2CID 4237513.
- PMID 1707519.
- S2CID 12709599.
- PMID 15574839.
- hdl:10261/163935.
- PMID 17977614.
- PMID 11741971.
- S2CID 46046514.
- PMID 15803656.
- S2CID 15246619.
- PMID 15229647.
- PMID 9707644.
- PMID 15611297.
- PMID 1379233.
- ^ S2CID 2509475.
- ^ PMID 18441046.
- ^ S2CID 4427070.
- ^ S2CID 9543041.
- S2CID 17907778.
- PMID 10618370.
- PMID 19390147.
- PMID 12606550.
- S2CID 4287636.
- S2CID 4343347.
- S2CID 619959.
- PMID 16672232.
- PMID 15588816.
Further reading
- Kuglstatter A, Oubridge C, Nagai K (October 2002). "Induced structural changes of 7SL RNA during the assembly of human signal recognition particle". Nature Structural Biology. 9 (10): 740–744. S2CID 9543041.
- Wang T, Tian C, Zhang W, Luo K, Sarkis PT, Yu L, Liu B, Yu Y, Yu XF (December 2007). "7SL RNA mediates virion packaging of the antiviral cytidine deaminase APOBEC3G". Journal of Virology. 81 (23): 13112–13124. PMID 17881443.
- Ullu E, Weiner AM (December 1984). "Human genes and pseudogenes for the 7SL RNA component of signal recognition particle". The EMBO Journal. 3 (13): 3303–3310. PMID 6084597.
- Englert M, Felis M, Junker V, Beier H (December 2004). "Novel upstream and intragenic control elements for the RNA polymerase III-dependent transcription of human 7SL RNA genes". Biochimie. 86 (12): 867–874. PMID 15667936.
- Oubridge C, Kuglstatter A, Jovine L, Nagai K (June 2002). "Crystal structure of SRP19 in complex with the S domain of SRP RNA and its implication for the assembly of the signal recognition particle". Molecular Cell. 9 (6): 1251–1261. PMID 12086622.
- Wang T, Tian C, Zhang W, Sarkis PT, Yu XF (January 2008). "Interaction with 7SL RNA but not with HIV-1 genomic RNA or P bodies is required for APOBEC3F virion packaging". Journal of Molecular Biology. 375 (4): 1098–1112. PMID 18067920.
External links
- The SRP Database (SRPDB): Alignments of SRP RNAs and associated proteins, SRP RNA secondary structures and 3-D models
- Rfam entry for Metazoan type signal recognition particle RNA
- Rfam entry for Bacterial small signal recognition particle RNA
- Rfam entry for Bacterial large signal recognition particle RNA
- Rfam entry for Fungal signal recognition particle RNA
- Rfam entry for Plant signal recognition particle RNA
- Rfam entry for Protozoan signal recognition particle RNA
- Rfam entry for Archaeal signal recognition particle RNA
- Dnatube Signal Recognition Particle Movie