Sterol regulatory element-binding protein

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Sterol regulatory element-binding transcription factor 1
Chr. 17 p11.2
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StructuresSwiss-model
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sterol regulatory element-binding transcription factor 2
Identifiers
SymbolSREBF2
Chr. 22 q13
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Sterol regulatory element-binding proteins (SREBPs) are

basic-helix-loop-helix leucine zipper class of transcription factors.[3] Unactivated SREBPs are attached to the nuclear envelope and endoplasmic reticulum membranes. In cells with low levels of sterols, SREBPs are cleaved to a water-soluble N-terminal domain that is translocated to the nucleus. These activated SREBPs then bind to specific sterol regulatory element DNA sequences, thus upregulating the synthesis of enzymes involved in sterol biosynthesis.[4][5]
Sterols in turn inhibit the cleavage of SREBPs and therefore synthesis of additional sterols is reduced through a negative feed back loop.

Isoforms

Mammalian genomes have two separate SREBP genes (SREBF1 and SREBF2):

  • SREBP-1 expression produces two different isoforms, SREBP-1a and -1c. These isoforms differ in their first exons owing to the use of different transcriptional start sites for the SREBP-1 gene. SREBP-1c was also identified in rats as ADD-1. SREBP-1c is responsible for regulating the genes required for de novo lipogenesis.[6]
  • SREBP-2 regulates the genes of cholesterol metabolism.[6]

Function

SREB proteins are indirectly required for

helix-loop-helix (HLH) proteins. However, in contrast to E-box-binding HLH proteins, an arginine residue is replaced with tyrosine making them capable of recognizing StREs and thereby regulating membrane biosynthesis.[7]

Mechanism of action

cholesterol synthesis
. SREBP processing can be controlled by the cellular sterol content.

Animal cells maintain proper levels of intracellular

enzymes
. Conversely, when there is enough cholesterol around, the cell stops making those mRNAs and the level of the enzymes falls. As a result, the cell quits making cholesterol once it has enough.

A notable feature of this regulatory feedback machinery was first observed for the SREBP pathway -

regulated intramembrane proteolysis
(RIP). Subsequently, RIP was found to be used in almost all organisms from bacteria to human beings and regulates a wide range of processes ranging from development to neurodegeneration.

A feature of the SREBP pathway is the proteolytic release of a membrane-bound transcription factor, SREBP. Proteolytic cleavage frees it to move through the cytoplasm to the nucleus. Once in the nucleus, SREBP can bind to specific DNA sequences (the sterol regulatory elements or SREs) that are found in the control regions of the genes that encode enzymes needed to make lipids. This binding to

genes
.

The ~120 kDa SREBP precursor protein is anchored in the membranes of the

S2P
).

In addition to S1P and S2P, the regulated release of transcriptionally active SREBP requires the cholesterol-sensing protein SREBP cleavage-activating protein (

eukaryotic cells
, subcellular compartmentalization defined by intracellular membranes, to ensure that cleavage occurs only when needed.

Once in the Golgi apparatus, the SREBP-SCAP complex encounters active S1P. S1P cleaves SREBP at site-1, cutting it into two halves. Because each half still has a membrane-spanning helix, each remains bound in the membrane. The newly generated amino-terminal half of SREBP (which is the ‘business end' of the molecule) then goes on to be cleaved at site-2 that lies within its membrane-spanning helix. This is the work of S2P, an unusual metalloprotease. This releases the cytoplasmic portion of SREBP, which then travels to the nucleus where it activates transcription of target genes (e.g. LDL receptor gene)

Regulation

Absence of sterols activates SREBP, thereby increasing cholesterol synthesis.[11]

Insulin, cholesterol derivatives, T3 and other endogenous molecules have been demonstrated to regulate the SREBP1c expression, particularly in rodents. Serial deletion and mutation assays reveal that both SREBP (SRE) and LXR (LXRE) response elements are involved in SREBP-1c transcription regulation mediated by insulin and cholesterol derivatives. Peroxisome proliferation-activated receptor alpha (PPARα) agonists enhance the activity of the SREBP-1c promoter via a DR1 element at -453 in the human promoter. PPARα agonists act in cooperation with LXR or insulin to induce lipogenesis.[12]

A medium rich in branched-chain amino acids stimulates expression of the SREBP-1c gene via the

mTORC1/S6K1 pathway. The phosphorylation of S6K1 was increased in the liver of obese db/db mice. Furthermore, depletion of hepatic S6K1 in db/db mice with the use of an adenovirus vector encoding S6K1 shRNA resulted in down-regulation of SREBP-1c gene expression in the liver as well as a reduced hepatic triglyceride content and serum triglyceride concentration.[13]

mTORC1 activation is not sufficient to stimulate hepatic SREBP-1c in the absence of Akt signaling, revealing the existence of an additional downstream pathway also required for this induction which is proposed to involve mTORC1-independent Akt-mediated suppression of INSIG-2a, a liver-specific transcript encoding the SREBP-1c inhibitor INSIG2.[14]

FGF21 has been shown to repress the transcription of sterol regulatory element binding protein 1c (SREBP-1c). Overexpression of FGF21 ameliorated the up-regulation of SREBP-1c and fatty acid synthase (FAS) in HepG2 cells elicited by FFAs treatment. Moreover, FGF21 could inhibit the transcriptional levels of the key genes involved in processing and nuclear translocation of SREBP-1c, and decrease the protein amount of mature SREBP-1c. Unexpectedly, overexpression of SREBP-1c in HepG2 cells could also inhibit the endogenous FGF21 transcription by reducing FGF21 promoter activity.[15]

SREBP-1c has also been shown to upregulate in a tissue specific manner the expression of PGC1alpha expression in brown adipose tissue.[16]

Nur77 is suggested to inhibit LXR and downstream SREBP-1c expression modulating hepatic lipid metabolism.[17]

History

The SREBPs were elucidated in the laboratory of Nobel laureates Michael Brown and Joseph Goldstein at the University of Texas Southwestern Medical Center in Dallas. Their first publication on this subject appeared in October 1993.[3][18]

References

  1. PMID 9634703
    .
  2. S2CID 26062629
    .
  3. ^
    S2CID 2784016
    .
  4. S2CID 44899129
    .
  5. S2CID 42988814
    .
  6. ^
    PMID 23859617
    .
  7. PMID 9634703
    .
  8. S2CID 17882616
    .
  9. PMID 10500120
    .
  10. S2CID 12194770
    .
  11. S2CID 18964672
    .
  12. PMID 21540177
    .
  13. PMID 21806970
    .
  14. PMID 21723501
    .
  15. S2CID 24001799
    .
  16. PMID 19962449
    .
  17. PMID 18086558
    .
  18. PMID 14507481
    .

External links
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