Substrate (biology)

In biology, a substrate is the surface on which an organism (such as a plant, fungus, or animal) lives. A substrate can include biotic or abiotic materials and animals. For example, encrusting algae that lives on a rock (its substrate) can be itself a substrate for an animal that lives on top of the algae. Inert substrates are used as growing support materials in the hydroponic cultivation of plants. In biology substrates are often activated by the nanoscopic process of substrate presentation.

In agriculture and horticulture

• Cellulose substrate^[1]
• Expanded clay aggregate (LECA)
• Rock wool
• Potting soil
• Soil

In animal biotechnology

Requirements for animal cell and tissue culture

Requirements for animal cell and tissue culture are the same as described for plant cell, tissue and organ culture (In Vitro Culture Techniques: The Biotechnological Principles). Desirable requirements are (i) air conditioning of a room, (ii) hot room with

There are many types of vertebrate cells that require support for their growth in vitro otherwise they will not grow properly. Such cells are called anchorage-dependent cells. Therefore, many substrates which may be adhesive (e.g. plastic, glass, palladium, metallic surfaces, etc.) or non-adhesive (e.g. agar, agarose, etc.) types may be used as discussed below:

• thermamox (TPX), polyvinylchloride (PVC), polycarbonate, etc. Monolayer of cell must be grown. Moreover, plastic beads of polystyrene, sephadex and polyacrylamide
  are also available for cell growth in suspension culture.
• Glass as a substrate. Glass is an important substrate used in laboratory in several forms such as test tubes, slides, coverslips, pipettes, flasks, rods, bottles, Petri dishes, several apparatus, etc. These are sterilized by using chemicals, radiations, dry heat (in oven) and moist heat (in autoclave).
• Palladium as a substrate. For the first time palladium deposited on agarose was used as a substrate for growth of fibroblast and glia. ^[2]

References

External links

• "Micro-vegetable growing" using abiotic substrates at home