Thyroid function tests
Thyroid function tests | |
---|---|
MeSH | D013960 |
MedlinePlus | 003444 |
Thyroid function tests (TFTs) is a collective term for blood tests used to check the function of the thyroid.[1] TFTs may be requested if a patient is thought to suffer from hyperthyroidism (overactive thyroid) or hypothyroidism (underactive thyroid), or to monitor the effectiveness of either thyroid-suppression or hormone replacement therapy. It is also requested routinely in conditions linked to thyroid disease, such as atrial fibrillation and anxiety disorder.
A TFT
Thyroid-stimulating hormone
Thyroid-stimulating hormone (TSH, thyrotropin) is generally increased in hypothyroidism and decreased in hyperthyroidism,[2] making it the most important test for early detection of both of these conditions.[3][4] The result of this assay is suggestive of the presence and cause of thyroid disease, since a measurement of elevated TSH generally indicates hypothyroidism, while a measurement of low TSH generally indicates hyperthyroidism.[2] However, when TSH is measured by itself, it can yield misleading results, so additional thyroid function tests must be compared with the result of this test for accurate diagnosis.[4][5][6]
TSH is produced in the pituitary gland. The production of TSH is controlled by thyrotropin-releasing hormone (TRH), which is produced in the hypothalamus. TSH levels may be suppressed by excess free T3 (fT3) or free T4 (fT4) in the blood.[citation needed]
History
First-generation TSH assays were done by radioimmunoassay and were introduced in 1965.[3] There were variations and improvements upon TSH radioimmunoassay, but their use declined as a new immunometric assay technique became available in the middle of the 1980s.[3][4] The new techniques were more accurate, leading to the second, third, and even fourth generations of TSH assay, with each generation possessing ten times greater functional sensitivity than the last.[7] Third generation immunometric assay methods are typically automated.[3] Fourth generation TSH immunometric assay has been developed for use in research.[4]
Current status
Third generation TSH assay is the current requirement for modern standards of care. At present, TSH testing in the United States is typically carried out with automated platforms using advanced forms of immunometric assay.[3] Nonetheless, there is currently no international standard for measurement of thyroid-stimulating hormone.[4]
Interpretation
Accurate interpretation takes a variety of factors into account, such as the thyroid hormones i.e.
Thyroid hormones
Total thyroxine
Total thyroxine is rarely measured, having been largely superseded by free thyroxine tests. Total
Total T4 is measured to see the bound and unbound levels of T4. The total T4 is less useful in cases where there could be protein abnormalities. The total T4 is less accurate due to the large amount of T4 that is bound. The total T3 is measured in clinical practice since the T3 has decreased amount that is bound as compared to T4.[citation needed]
Reference ranges depend on the method of analysis. Results should always be interpreted using the range from the laboratory that performed the test. Example values are:
Lower limit | Upper limit | Unit |
4,[11] 5.5[12] | 11,[11] 12.3[12] | μg/dL |
60[11][13] | 140,[11] 160[13] | nmol/L |
Free thyroxine
Free
Reference ranges depend on the method of analysis. Results should always be interpreted using the range from the laboratory that performed the test. Example values are:
Patient type | Lower limit | Upper limit | Unit |
Normal adult | 0.7,[14] 0.8[12] | 1.4,[14] 1.5,[12] 1.8[15] | ng/dL |
9,[16][17] 10,[11] 12 [13] | 18,[16][17] 23[13] | pmol/L | |
Infant 0–3 d | 2.0[14] | 5.0[14] | ng/dL |
26[17] | 65[17] | pmol/L | |
Infant 3–30 d | 0.9[14] | 2.2[14] | ng/dL |
12[17] | 30[17] | pmol/L | |
Child/Adolescent 31 d – 18 y |
0.8[14] | 2.0[14] | ng/dL |
10[17] | 26[17] | pmol/L | |
Pregnant | 0.5[14] | 1.0[14] | ng/dL |
6.5[17] | 13[17] | pmol/L |
Total triiodothyronine
Total triiodothyronine (Total T3) is rarely measured, having been largely superseded by free T3 tests. Total T3 is generally elevated in hyperthyroidism and decreased in hypothyroidism.[2]
Reference ranges depend on the method of analysis. Results should always be interpreted using the range from the laboratory that performed the test. Example values are:
Test | Lower limit | Upper limit | Unit |
Total triiodothyronine | 60,[12] 75[11] | 175,[11] 181[12] | ng/dL |
0.9,[16] 1.1[11] | 2.5,[16] 2.7[11] | nmol/L |
Free triiodothyronine
Free triiodothyronine (fT3 or free T3) is generally elevated in hyperthyroidism and decreased in hypothyroidism.[2]
Reference ranges depend on the method of analysis. Results should always be interpreted using the range from the laboratory that performed the test. Example values are:
Patient type | Lower limit | Upper limit | Unit |
Normal adult | 3.0[11] | 7.0[11] | pg/mL |
3.1[18] | 7.7[18] | pmol/L | |
Children 2–16 y | 3.0[19] | 7.0[19] | pg/mL |
1.5[18] | 15.2[18] | pmol/L |
Carrier proteins
Thyroxine-binding globulin
An increased thyroxine-binding globulin results in an increased total thyroxine and total triiodothyronine without an actual increase in hormonal activity of thyroid hormones.
Reference ranges:
Lower limit | Upper limit | Unit |
12[12] | 30[12] | mg/L |
Thyroglobulin
Reference ranges:
Lower limit | Upper limit | Unit |
1.5[11] | 30[11] | pmol/L |
1[11] | 20 [11] | μg/L |
Other binding hormones
- Transthyretin (prealbumin)
- Albumin
Protein binding function
Thyroid hormone uptake
Thyroid hormone uptake (Tuptake or T3 uptake) is a measure of the unbound
Reference ranges:
Patient type | Lower limit | Upper limit | Unit |
Females | 25[2] | 35[2] | % |
In pregnancy | 15[2] | 25[2] | % |
Males | 25[2] | 35[2] | % |
Other protein binding tests
- Thyroid Hormone Binding Ratio (THBR)
- Thyroxine-binding index (TBI)
Mixed parameters
Free thyroxine index
The Free Thyroxine Index (FTI or T7) is obtained by multiplying the total T4 with T3 uptake.[2] FTI is considered to be a more reliable indicator of thyroid status in the presence of abnormalities in plasma protein binding.[2] This test is rarely used now that reliable free thyroxine and free triiodothyronine assays are routinely available.
FTI is elevated in hyperthyroidism and decreased in hypothyroidism.[2]
Patient type | Lower limit | Upper limit | Unit |
Females | 1.8[2] | 5.0[2] | |
Males | 1.3[2] | 4.2[2] |
Calculated and structure parameters
Derived
Secretory capacity (GT)
Thyroid's secretory capacity (GT, also referred to as SPINA-GT) is the maximum stimulated amount of thyroxine the thyroid can produce in one second.[24] GT is elevated in hyperthyroidism and reduced in hypothyroidism.[25]
GT is calculated with
or
: Dilution factor for T4 (reciprocal of apparent volume of distribution, 0.1 l−1)
: Clearance exponent for T4 (1.1e-6 sec−1)
K41: Dissociation constant T4-TBG (2e10 L/mol)
K42: Dissociation constant T4-TBPA (2e8 L/mol)
DT: EC50 for TSH (2.75 mU/L)[24]
Lower limit | Upper limit | Unit |
1.41[24] | 8.67[24] | pmol/s |
Sum activity of peripheral deiodinases (GD)
The sum activity of peripheral deiodinases (GD, also referred to as SPINA-GD) is reduced in nonthyroidal illness with hypodeiodination.[21][22][26]
GD is obtained with
or
: Dilution factor for T3 (reciprocal of apparent volume of distribution, 0.026 L−1)
: Clearance exponent for T3 (8e-6 sec−1)
KM1: Dissociation constant of type-1-deiodinase (5e-7 mol/L)
K30: Dissociation constant T3-TBG (2e9 L/mol)[24]
Lower limit | Upper limit | Unit |
20[24] | 40[24] | nmol/s |
TSH index
It is calculated with
.
Additionally, a standardized form of TSH index may be calculated with
.[27]
Parameter | Lower limit | Upper limit | Unit |
TSHI | 1.3[27] | 4.1[27] | |
sTSHI | -2[27] | 2[27] |
TTSI
The Thyrotroph Thyroid Hormone Sensitivity Index (TTSI, also referred to as Thyrotroph T4 Resistance Index or TT4RI) was developed to enable fast screening for resistance to thyroid hormone.[28][29] Somewhat similar to the TSH Index it is calculated from equilibrium values for TSH and FT4, however with a different equation.
Lower limit | Upper limit | Unit |
100 | 150 |
TFQI
The Thyroid Feedback Quantile-based Index (TFQI) is another parameter for thyrotropic pituitary function. It was defined to be more robust to distorted data than JTI and TTSI. It is calculated with
from quantiles of FT4 and TSH concentration (as determined based on
Lower limit | Upper limit | Unit |
–0,74 | +0.74 |
Reconstructed set point
In healthy persons, the intra-individual variation of TSH and thyroid hormones is considerably smaller than the inter-individual variation.[39][40][41] This results from a personal set point of thyroid homeostasis.[42] In hypothyroidism, it is impossible to directly access the set point,[43] but it can be reconstructed with methods of systems theory.[44][45][46]
A computerised algorithm, called Thyroid-SPOT, which is based on this mathematical theory, has been implemented in software applications.[47] In patients undergoing thyroidectomy it could be demonstrated that this algorithm can be used to reconstruct the personal set point with sufficient precision.[48]
Effects of drugs
Drugs can profoundly affect thyroid function tests. Listed below is a selection of important effects.
Cause | Drug | Effect on hormone concentrations | Effect on structure parameters |
---|---|---|---|
Inhibited TSH secretion | glucocorticoids, somatostatin |
↓T4; ↓T3; ↓TSH | ↔SPINA-GT; ↓JTI |
Inhibited synthesis or release of thyroid hormone | Iodine, lithium | ↓T4; ↓T3; ↑TSH | ↓SPINA-GT; ↔JTI |
Inhibited conversion of T4 to T3 (Step-up hypodeiodination) | radiographic contrast agents |
↓T3; ↑rT3; ↓, ↔, ↑T4 and fT4; ↔, ↑TSH | ↓SPINA-GD |
Inhibited binding of T4/T3 to serum proteins | nonsteroidal anti-inflammatory agents, heparin (in vitro effect) |
↓T4; ↓T3; ↓fT4E, ↔, ↑fT4; ↔TSH | ↓T4/fT4 ratio |
Stimulated metabolism of iodothyronines | Phenobarbital, phenytoin, carbamazepine, rifampicin | ↓T4; ↓fT4; ↔TSH | |
Inhibited absorption of ingested T4 | kayexalate |
↓T4; ↓fT4; ↑TSH | |
Increase in concentration of T4-binding proteins | 5-fluorouracil, perphenazine |
↑T4; ↑T3; ↔fT4; ↔TSH | ↔SPINA-GT; ↔SPINA-GD; ↔JTI; ↑T4/fT4 ratio |
Decrease in concentration of T4-binding proteins | glucocorticoids |
↓T4; ↓T3; ↔fT4; ↔TSH | ↔SPINA-GT; ↔SPINA-GD; ↔JTI; ↓T4/fT4 ratio |
↓: reduced serum concentration or structure parameter; ↑: increased serum concentration or structure parameter; ↔: no change; TSH: Thyroid-stimulating hormone; T3: Total triiodothyronine; T4: Total thyroxine; fT4: Free thyroxine; fT3: Free triiodothyronine; rT3: Reverse triiodothyronine
See also
- Long-acting thyroid stimulator(LATS)
References
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- ^ a b c d e f g h i j k l m n o p q r s t u v Military Obstetrics & Gynecology > Thyroid Function Tests In turn citing: Operational Medicine 2001, Health Care in Military Settings, NAVMED P-5139, May 1, 2001, Bureau of Medicine and Surgery, Department of the Navy, 2300 E Street NW, Washington, D.C., 20372-5300 "Normal Reference Range Table". Archived from the original on 25 December 2011. Retrieved 2011-12-25.
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- ^ a b c d e f g h i j k l m n o Table 4: Typical reference ranges for serum assays Archived July 1, 2011, at the Wayback Machine - Thyroid Disease Manager
- ^ a b c d e f g h Normal Reference Range Table Archived 2011-12-25 at the Wayback Machine from The University of Texas Southwestern Medical Center at Dallas. Used in Interactive Case Study Companion to Pathologic basis of disease.
- ^ PMID 18593963.
- ^ a b c d e f g h i j Free T4; Thyroxine, Free; T4, Free Archived 2010-12-22 at the Wayback Machine UNC Health Care System
- ^ Derived from molar values using molar mass of 776.87 g/mol
- ^ a b c d Reference range list from Uppsala University Hospital ("Laborationslista"). Artnr 40284 Sj74a. Issued on April 22, 2008
- ^ a b c d e f g h i j Derived from mass values using molar mass of 776.87 g/mol
- ^ a b c d Derived from mass values using molar mass of 650.98 g/mol
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- ^ Dietrich, J., M. Fischer, J. Jauch, E. Pantke, R. Gärtner und C. R. Pickardt (1999). "SPINA-THYR: A Novel Systems Theoretic Approach to Determine the Secretion Capacity of the Thyroid Gland." European Journal of Internal Medicine 10, Suppl. 1 (5/1999): S34.
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- ^ "甲状腺素抵抗与糖尿病和代谢综合征有关?看TFQI怎么说". www.medinfo-sanofi.cn. Retrieved 14 April 2020.[permanent dead link]
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Further reading
- American Thyroid Association: Thyroid Function Tests. Posted on June 4, 2012, seen on January 9, 2013.
- Thyroid function panel - Lab Tests Online
External links
- SPINA Thyr: Open source software for calculating GT and GD
- Interpretation of thyroid function tests by Dayan, Colin M. 2001. The Lancet, Vol. 357.
CDC laboratory procedure manuals
The Centers for Disease Control and Prevention has published the following laboratory procedure manuals for measuring thyroid-stimulating hormone:
- Thyroid Stimulating Hormone (TSH) (University of Washington Medical Center). September 2011. Method: Access 2 (Beckman Coulter).
- Thyroid Stimulating Hormone (TSH) (Collaborative Laboratory Services). September 2011. Method: Access 2 (Beckman Coulter).
- Thyroid Stimulating Hormone (TSH). September 2009. Method: Access 2 (Beckman Coulter).
- Lab 18 Thyroid Stimulating Hormone. 2001-2002. Method: Microparticle Enzyme Immunoassay.
- Lab 18 TSH - Thyroid Stimulating Hormone. 1999-2000. Method: Microparticle Enzyme Immunoassay.