Trans-activating crRNA
In
transcribed from this CRISPR locus. The crRNAs are then incorporated into effector complexes, where the crRNA guides the complex to the invading nucleic acid and the Cas proteins degrade this nucleic acid.[2]
There are several CRISPR system subtypes.
Type II CRISPR-Cas systems require a tracrRNA which plays a role in the maturation of crRNA.[3] The tracrRNA is partially
RNase III, an RNA-specific ribonuclease, to form a crRNA/tracrRNA hybrid. This hybrid acts as a guide for the endonuclease Cas9, which cleaves the invading nucleic acid.[1][4][5]
See also
References
Further reading
- Le Rhun A, Charpentier E (2012). "Small RNAs in streptococci". RNA Biol. 9 (4): 414–426. PMID 22546939.