Tubuloglomerular feedback

Source: Wikipedia, the free encyclopedia.

In the

purinergic signaling, in which an increased distal tubular sodium chloride concentration causes a basolateral release of adenosine from the macula densa cells. This initiates a cascade of events that ultimately brings GFR to an appropriate level.[1][2][3]

Background

The kidney maintains the electrolyte concentrations, osmolality, and acid-base balance of blood plasma within the narrow limits that are compatible with effective cellular function; and the kidney participates in blood pressure regulation and in the maintenance of steady whole-organism water volume [4]

Fluid flow through the nephron must be kept within a narrow range for normal renal function in order to not compromise the ability of the nephron to maintain salt and water balance.[5] Tubuloglomerular feedback (TGF) regulates tubular flow by detecting and correcting changes in GFR. Active transepithelial transport is used by the thick ascending limb of loop of Henle (TAL) cells to pump NaCl to the surrounding interstitium from luminal fluid. The tubular fluid is diluted because the cell's walls are water-impermeable and do not lose water as NaCl is actively reabsorbed. Thus, the TAL is an important segment of the TGF system, and its transport properties allow it to act as a key operator of the TGF system.[5] A reduction of GFR occurs as a result of TGF when NaCl concentration at the sensor site is increased within the physiological range of approximately 10 to 60 mM.[6]

The TGF mechanism is a negative feedback loop in which the chloride ion concentration is sensed downstream in the nephron by the macula densa (MD) cells in the tubular wall near the end of TAL and the glomerulus. The muscle tension in the afferent arteriole is modified based on the difference between the sensed concentration and a target concentration.[5] Vasodilation of the afferent arteriole, which results in increased glomerular filtration pressure and tubular fluid flow, occurs when MD cells detect a chloride concentration that is below a target value. A higher fluid flow rate in the TAL allows less time for dilution of the tubular fluid so that MD chloride concentration increases.[5] Glomerular flow is decreased if the chloride concentration is above the target value. Constricting the smooth muscle cells in the afferent arteriole, results in a reduced concentration of chloride at the MD. TGF stabilizes the fluid and solute delivery into the distal portion of the loop of Henle and maintain the rate of filtration near its ideal value using these mechanisms.

Mechanism

The

afferent and efferent arterioles
. The macula densa's position enables it to rapidly alter afferent arteriolar resistance in response to changes in the flow rate through the distal nephron.

The macula densa uses the composition of the tubular fluid as an indicator of GFR. A large sodium chloride concentration is indicative of an elevated GFR, while low sodium chloride concentration indicates a depressed GFR. Sodium chloride is sensed by the macula densa mainly by an apical

loop diuretics like furosemide.[7]
Furosemide blocks NaCl reabsorption mediated by the NKCC2 at the ascending loop of henle, which leads to increased renin release. Excluding loop diuretic use, the usual situation that causes a reduction in reabsorption of NaCl via the NKCC2 at the macula densa (DCT) is a low tubular lumen concentration of NaCl due to low GFR. Reduced NaCl uptake via the NKCC2 at the macula densa leads to increased renin release, which leads to restoration of plasma volume, and to dilation of the afferent arterioles, which leads to increased renal plasma flow and increased GFR.

The macula densa's detection of elevated sodium chloride concentration in the tubular lumen, which leads to a decrease in GFR, is based on the concept of

purinergic signaling.[1][2][8]

In response to increased flow of tubular fluid in the thick ascending limb/ increased sodium chloride (salt) concentration at the macula densa:

  1. Elevated filtration at the glomerulus or reduced reabsorption of sodium and water by the
    Proximal Convoluted Tubule
    causes the tubular fluid at the macula densa to have a higher concentration of sodium chloride.
  2. Apical
    Na-K-2Cl cotransporters
    (NKCC2), which are found on the surface of the macula densa cells, are exposed to the fluid with a higher sodium concentration, and as a result more sodium is transported into the cells.
  3. The macula densa cells do not have enough
    osmolarity
    .
  4. Water flows into the cell along the osmotic gradient, causing the cell to swell. When the cell swells, ATP escapes through a basolateral, stretch-activated, non-selective Maxi-Anion channel.[9] The ATP is subsequently converted to adenosine by ecto-5′-nucleotidase.[10]
  5. Adenosine constricts the afferent arteriole by binding with high affinity to the A1 receptors[11][12] a Gi/Go. Adenosine binds with much lower affinity to A2A and A2B[13] receptors causing dilation of efferent arterioles.[12]
  6. The binding of adenosine to the
    gap junctions creating a "TGF calcium wave".[10]
    This causes afferent arteriolar vasoconstriction, decreasing the glomerular filtrate rate.
  7. The Gi and increased intracellular calcium, cause a decrease in cAMP which inhibits Renin release from the juxtaglomerular cells.[14] In addition, when macula densa cells detect higher concentrations of Na and Cl, they inhibit nitric oxide synthetase (decreasing renin release), but the most important inhibitory mechanism of renin synthesis and release is elevations in juxtaglomerular cell calcium concentration.[7]

In response to decreased flow of tubular fluid in the thick ascending limb / decreased salt concentration at the macula densa:

  1. Reduced filtration at the glomerulus or increased reabsorption of sodium and water by the
    Proximal Convoluted Tubule
    causes fluid in the tubule at the macula densa to have a reduced concentration of sodium chloride.
  2. NKCC2 has a lower activity and subsequently causes a complicated signaling cascade involving the activation of: p38,
    (COX-2) and microsomal prostaglandin E synthase (mPGES) in the macula densa.[7]
  3. This causes the synthesis and release of
    PGE2
    .
  4. PGE2 acts on EP2 and EP4 receptors in juxtaglomerular cells and causes renin release.[7]
  5. Renin release activates RAAS leading to many outcomes including an increased GFR.

The critical target of the trans-JGA signaling cascade is the glomerular afferent arteriole; its response consists of an increase in net vasoconstrictor tone resulting in reductions of glomerular capillary pressure (PGC) and glomerular plasma flow. Efferent arterioles appear to play a lesser role; experimental evidence supports both vasoconstriction and vasodilation, with perhaps the former in the lower range and the latter in the higher range of NaCl concentrations (2). When feedback regulation of afferent arteriolar tone is prevented by interrupting the feedback loop, and when the sensing mechanism is fully activated by saturating NaCl concentrations, TGF reduces GFR on average by approximately 45% and PGC by approximately 20%. Afferent arteriolar resistance increases by 50% or less, consistent with a radius reduction of approximately 10%, if Poiseuille's law holds. Thus, TGF-induced vasoconstriction is usually limited in magnitude.[6]

Modulation

A mediating agent is released or generated as a function of changes in luminal NaCl concentration. The size of the TGF response is directly dependent upon these changes. "In part because of the striking effect of deletion of A1 adenosine receptors (A1AR), adenosine generated from released ATP has been proposed as the critical TGF mediator.[6] A modulating agent affects the TGF response without input regarding luminal NaCl. The agents are vasoactive substances that alter either the magnitude or the sensitivity of the TGF response.[6]

Factors that decrease TGF sensitivity include:[15]

The threshold at which the loop of Henle flow rate initiates feedback responses is affected. A high protein diet affects the feedback activity by making the single nephron glomerular filtration rate higher, and the Na and Cl concentrations in early distal tubule fluid lower. The signal eliciting the TG feedback response is affected. The increased load on the kidney of

reabsorption of NaCl.[17]

  • Connecting tubule glomerular feedback (CTGF): CTGF is initiated by increased sodium concentration at the connecting tubule segment of the nephron and involves the activation of epithelial sodium channel (ENaC). CTGF has the ability to modulate TGF mechanism and is critical in understanding renal damage observed in salt sensitive hypertension[18][19] and kidney donors.[20]

Factors that increase TGF sensitivity include:[15]

  • adenosine
  • thromboxane
  • 5-HETE
  • angiotensin II
  • prostaglandin E2
  • aldosterone
    • Aldosterone within the connecting tubule lumen enhances connecting tubule glomerular feedback (CTGF) via a nongenomic effect involving GPR30 receptors and sodium/hydrogen exchanger (NHE). Aldosterone inside the connecting tubule lumen enhances CTGF via a cAMP/PKA/PKC pathway and stimulates O2− generation and this process may contribute to renal damage by increasing glomerular capillary pressure.[21]

See also

References

  1. ^
    PMID 23908631
    . Extracellular adenosine contributes to the regulation of GFR. Renal interstitial adenosine is mainly derived from dephosphorylation of released ATP, AMP, or cAMP by the enzyme ecto-5′-nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine or inosine, respectively, and is located primarily on the external membranes and mitochondria of proximal tubule cells, but not in distal tubule or collecting duct cells (Miller et al., 1978). ATP consumed in active transport by the macula densa also contributes to the formation of adenosine by 5- nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth muscle cells, resulting in vasoconstriction and a reduction in GFR (Schnermann et al., 1990).
  2. ^
    PMID 20148681
    .
  3. PMID 23882224
    .
  4. ^ Widmaier EP, Raff H, Strang KT (2016). Vander's Human Physiology: The Mechanisms of Body Function. New York, NY: McGraw-Hill Education.
  5. ^
    PMID 23529284
    .
  6. ^
    PMID 25668021
    .
  7. ^
    PMID 20360309
    .
  8. PMID 20519378
    .
  9. S2CID 15170688
    .
  10. ^
    PMID 24265071
    .
  11. PMID 1991645
    .
  12. ^
    PMID 19639291
    .
  13. PMID 20462966
    .
  14. ^
    PMID 23884142
    .
  15. ^
    ISBN 978-1-4160-2328-9
    .
  16. PMID 25089004
    .
  17. PMID 3812704
    .
  18. PMID 23959547
    .
  19. PMID 28835421
    .
  20. PMID 28424211
    .
  21. PMID 27413197
    .

Further reading

Retrieved from "https://en.wikipedia.org/w/index.php?title=Tubuloglomerular_feedback&oldid=1192846950"