Vero cell
(Redirected from
Vero cells
)Vero cells are a lineage of
Chiba, Japan.[2] The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto.[3]
Characteristics
The Vero cell lineage is continuous and
senescent.[4]
Vero cells are interferon-deficient; unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses.[5] However, they still have the Interferon-alpha/beta receptor, so they respond normally when recombinant interferon is added to their culture media.
The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014.Chlorocebus sabaeus.[6]
Uses in research
Vero cells are used for many purposes, including:
- screening for the toxin of
- as host cells for growing viruses; for example, to measure replication in the presence or absence of a research pharmaceutical, the testing for the presence of rabies virus, or the growth of viral stocks for research purposes. As a recent example, CoronaVac, COVID-19 vaccine developed by Sinovac Biotech uses vero cells in production and "Vero" term can be seen on the vaccine container.
- as host cells for trypanosomatids[6]
Lineages
- Vero (ATCC No. CCL-81)
- Isolated from C. aethiops kidney on 27 Mar 1962
- Vero 76 (ATCC No. CRL-1587)
- Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. It is useful for detecting and counting hemorrhagic fever viruses by plaque assays.
- Vero E6, also known as Vero C1008 (ATCC No. CRL-1586)
- This line is a clone from Vero 76. Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly.
- Research strains transfected with viral genes:
- Vero F6 is a cell transfected with the gene encoding promoterregion. In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (Also F6B2; obs. F6B1.1)
See also
References
- ^ History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf
- ^ Yasumura Y, Kawakita M (1963). "The research for the SV40 by means of tissue culture technique". Nippon Rinsho. 21 (6): 1201–1219.
- ISBN 978-4-320-05386-1.
- ^ "Main Types of Cell Culture". Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved 2006-09-28.
- PMID 4302013.
- ^ PMID 25267831.
- PMID 23307319.
- PMID 1309250.
External links
Wikimedia Commons has media related to Vero cells.