Vero cell

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Vero cells
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Phase-contrast microscopic image of Vero cells (under green light at 100-fold magnification)

Vero cells are a lineage of

Chiba, Japan.[2] The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto.[3]

Characteristics

The Vero cell lineage is continuous and

Vero cells are interferon-deficient; unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses.[5] However, they still have the Interferon-alpha/beta receptor, so they respond normally when recombinant interferon is added to their culture media.

The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014.

Chlorocebus sabaeus.[6]

Uses in research

Vero cells are used for many purposes, including:

Lineages

Isolated from C. aethiops kidney on 27 Mar 1962
Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. It is useful for detecting and counting
hemorrhagic fever viruses by plaque assays
.
This line is a clone from Vero 76. Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly.
  • Research strains transfected with viral genes:
Vero F6 is a cell transfected with the gene encoding
promoter
region. In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (Also F6B2; obs. F6B1.1)

See also

References

  1. ^ History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf
  2. ^ Yasumura Y, Kawakita M (1963). "The research for the SV40 by means of tissue culture technique". Nippon Rinsho. 21 (6): 1201–1219.
  3. .
  4. ^ "Main Types of Cell Culture". Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved 2006-09-28.
  5. PMID 4302013
    .
  6. ^ .
  7. .
  8. .

External links