Differential dynamic microscopy

Differential dynamic microscopy (DDM) is an optical technique that allows performing

The typical DDM data is a time sequence of microscope images (movie) acquired at some height within the sample (typically at its mid-plane). If the image intensity is locally proportional to the concentration of particles or molecules to be studied (possibly convoluted with the microscope point spread function (PSF)), each movie can be analyzed in the Fourier space to obtain information about the dynamics of concentration Fourier modes, independent on the fact that the particles/molecules can be individually optically resolved or not. After suitable calibration also information about the Fourier amplitude of the concentration modes can be extracted.

Applicability and working principle

The concentration-intensity proportionality is valid at least in two very important cases that distinguish two corresponding classes of DDM methods:

1. scattering-based DDM: where the image is the result of the superposition of the strong transmitted beam with the weakly scattered light from the particles. Typical cases where this condition can be obtained are
   phase contrast, polarized
   microscopes.
2. fluorescence-based DDM: where the image is the result of the incoherent addition of the intensity emitted by the particles (
   fluorescence, confocal
   ) microscopes

DDM is based on an algorithm proposed in Croccolo et al.[3] and Alaimo et al.,^[4] which is conveniently named differential dynamic algorithm (DDA). DDA works by subtracting images acquired at different times and taking advantage that, as the delay ${\displaystyle \Delta t}$ between two subtracted images gets large, the energy content of the difference image increases correspondingly. A two-dimensional fast Fourier transform (FFT) analysis of the difference images allows to quantify the growth of the signal contains for each wave vector ${\displaystyle q}$ and one can calculate the Fourier power spectrum of the difference images for different delays ${\displaystyle \Delta t}$ to obtain the so-called image structure function ${\displaystyle D(q;\Delta t)}$. Calculation shows that for both scattering- and fluorescence-based DDM

${\displaystyle D(q;\Delta t)=B(q)+T(q)I(q)[1-f(q;\Delta t)]\,}$

(1)

where ${\displaystyle f(q;\Delta t)}$ is the normalized

experiment, ${\displaystyle I(q)}$ the sample scattering intensity that would be measured in a

${\displaystyle B(q)}$

${\displaystyle T(q)}$

intermediate scattering function is available.^[2] For instance, in the case of Brownian motion

one has ${\displaystyle f(q;\Delta t)=e^{-Dq^{2}\Delta t},}$ where ${\displaystyle D}$ is the diffusion coefficient of the Brownian particles. If the transfer function ${\displaystyle T(q)}$ is determined by calibrating the microscope with a suitable sample, DDM can be employed also for SLS experiments. Alternative algorithms for data analysis are suggested in.^[2]

Running DDM on a series of frames smaller than the full-frame has been called multi-DDM. This is analogous to changing the scattering volume in a light scattering experiment, but is readily carried out by selecting out of the full-frame movie. The coherence lengthscale of the dynamics can be picked up from a multi-DDM analysis.^[5]

Relationship with other imaging-based scattering methods

Scattering-based DDM belongs to the so-called near-field (or deep Fresnel) scattering family,

near field speckles i.e. as a particular case of Fresnel region as opposed to the far field or Fraunhofer region. The near field scattering family includes also quantitative shadowgraphy^[9] and Schlieren.^[3]