Elution

Source: Wikipedia, the free encyclopedia.
Not to be confused with elutriation.
Elution principle of column chromatography

In

biopolymers from a gel electrophoresis or chromatography column
.

In a liquid

detector
or is collected by a fraction collector for compositional analysis.

Predicting and controlling the order of elution is a key aspect of column chromatographic and column electrophoretic methods.

Eluotropic series

An eluotropic series is listing of various compounds in order of eluting power for a given

stationary phase
as well as on the compound used to determine the order.

Adsorption strength (least strongly adsorbed → most strongly adsorbed)
Saturated hydrocarbons; alkyl halides Unsaturated hydrocarbons; alkenyl halides Aromatic hydrocarbons; aryl halides Polyhalogenated hydrocarbons Ethers Esters Aldehydes and ketones Alcohols Acids and bases (amines)
Eluting power (least eluting power → greatest eluting power)
Hexane or pentane Cyclohexane Benzene Dichloromethane Chloroform Ether (anhydrous) Ethyl acetate (anhydrous) Acetone (anhydrous) Methanol Ethanol Pyridine Acetic acid Water

Eluent

The eluent or eluant is the "carrier" portion of the mobile phase. It moves the analytes through the

liquid chromatography, the eluent is the liquid solvent; in gas chromatography, it is the carrier gas.[1]

Eluate

The eluate contains the analyte material that emerges from the chromatograph. It specifically includes both the analytes and coeluting solutes passing through the column, while the eluent is only the carrier.

Elution time and elution volume

The "elution time" of a solute is the time between the start of the separation (the time at which the solute enters the column) and the time at which the solute elutes. In the same way, the elution volume is the volume of eluent required to cause elution. Under standard conditions for a known mix of solutes in a certain technique, the elution volume may be enough information to identify solutes. For instance, a mixture of

ion-exchange chromatography
. Under a particular set of conditions, the amino acids will elute in the same order and at the same elution volume.

Antibody elution

Antibody elution is the process of removing antibodies that are attached to their targets, such as the surface of red blood cells. Techniques include using heat, a freeze-thaw cycle, ultrasound, acids or organic solvents. No single method is best in all situations.[2]

See also

References

  1. doi:10.1351/goldbook.E02040. Retrieved 2008-09-28. {{cite journal}}: Cite journal requires |journal= (help
    )
  2. ^ George H. Roberts (2006). "Elution Techniques in Blood Bank" (PDF). American Medical Technologists (AMT). Archived from the original (PDF) on 2019-07-11. Retrieved 2020-07-22.
  • Brown, Phillis (2001). Advances in chromatography.
    ISBN 0-8247-0509-2
    .

External links
Retrieved from "https://en.wikipedia.org/w/index.php?title=Elution&oldid=1219522644"