HL60
The HL-60 cell line is a human
MD Anderson Cancer Center.[1] HL-60 cells predominantly show neutrophilic promyelocytic morphology.[1] Subsequent evaluation, including the karyotype that showed absence of the defining t(15;17) translocation, concluded that HL-60 cells are from a case of AML FAB-M2 (now referred to as AML with maturation (WHO)).[2]
Proliferation of HL-60 cells occurs through the
monocytic, macrophage-like and eosinophil
phenotypes, respectively.
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. HL-60 cell model was used to study the effect of
DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells[4] and is especially useful in dielectrophoresis studies,[5] which require an aqueous environment with suspended and round cells. Furthermore, these cells have been used in order to investigate whether intracellular calcium plays a role in caspase activation induced by reactive oxygen species.[6]
Chromatin and gene expression profiling in HL-60 cells and differentiated cells derived from these has been performed recently.[7]
References
- ^ PMID 288488.[permanent dead link]
- PMID 3422031.
- PMID 6988226.)
{{cite journal}}: CS1 maint: multiple names: authors list (link - PMID 9454772.)
{{cite journal}}: CS1 maint: multiple names: authors list (link - PMID 12175928.)
{{cite journal}}: CS1 maint: multiple names: authors list (link - doi:10.2174/157436210791112172.)
{{cite journal}}: CS1 maint: multiple names: authors list (link - PMID 28406749.)
{{cite journal}}: CS1 maint: multiple names: authors list (link
External links
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