Hin recombinase
| DNA-invertase hin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | hin | ||||||
UniProt | P03013 | ||||||
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Hin recombinase is a 21kD
flagellar genes, fljA and fljB. Inversion of the intervening DNA alternates the direction of the promoter and thereby alternates expression of the flagellar genes. This is advantageous to the bacterium as a means of escape from the host immune response
.
Hin functions by binding to two 26bp imperfect inverted repeat sequences as a
Fis
proteins binds with nanomolar affinity. Four molecules of Fis bind to this site as a homodimers and are required for the recombination reaction to proceed.
The initial reaction requires binding of Hin and Fis to their respective DNA sequences and assemble into a higher-order
hydrophobic
interface that allows for subunit rotation which may be driven by superhelical torsion within the protein-DNA complex. After this 180° rotation, Hin returns to its native conformation and re-ligates the cleaved DNA, without the aid of high energy cofactors and without the loss of any DNA.
References
- Dhar G, Sanders E, Johnson R (2004). "Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands". Cell. 119 (1): 33–45. PMID 15454079.
- Sanders E, Johnson R (2004). "Stepwise dissection of the Hin-catalyzed recombination reaction from synapsis to resolution". J Mol Biol. 340 (4): 753–66. PMID 15223318.
- Kamtekar S, Ho R, Cocco M, Li W, Wenwieser S, Boocock M, Grindley N, Steitz T (2006). "Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination". Proc Natl Acad Sci USA. 103 (28): 10642–7. PMID 16807292.
- Li W, Kamtekar S, Xiong Y, Sarkis G, Grindley N, Steitz T (2005). "Structure of a synaptic gammadelta resolvase tetramer covalently linked to two cleaved DNAs". Science. 309 (5738): 1210–5. S2CID 84409916.