Hypothetical protein
In
homologue in the protein database
, it returns "hypothetical protein" as an annotation remark.
The function of a hypothetical protein can be predicted by
homology searches with various confidence levels.[2] Conserved domains are available in the hypothetical proteins which need to be compared with the known family domains by which hypothetical protein could be classified into particular protein families even though they have not been in vivo investigated. The function of hypothetical protein could also be predicted by homology modelling, in which hypothetical protein has to align with known protein sequence whose three dimensional structure is known and by modelling method if structure predicted then the capability of hypothetical protein to function could be ascertained computationally.[2][3][4] Further, approaches to annotate function to hypothetical proteins include determination of 3-dimensional structure of these proteins by structural genomics initiatives, understanding the nature and mode of prosthetic group/metal ion binding, fold similarity with other proteins of known functions and annotating possible catalytic site and regulatory site.[5] Structure prediction with biochemical function assessment by screening for various substrate is another promising approach to annotate function[2]
See also
References
- PMID 18628897.
- ^ PMID 25655405.
- ^ P S Kewate; R C Urade; D G Gore; M A Soni; A P Kopulwar (2015). "In silico enzyme function prediction in hypothetical proteins of Mycobacterium bovis AF2122/97". Journal of Pharmacy Research. 9 (3): 182–189.
- ^ Dilip Gore (2009). "In silico Prediction of Structure and Enzymatic Activity for Hypothetical Proteins of Shigellaflexneri. Biofrontiers". Biofrontiers. 1 (2): 1–10.
- PMID 10679350.
- Sunil Pande Dilip Gore (2015). "Does hypothetical proteins of Yersinia pestis CO92 Capable of Coding Enzymes?". Journal of Pharmacy Research. 9: 278–287.
- Dilip Gore Ashish Chakule (2012). "Homology modeling and function prediction in uncharacterized proteins of Pseudoxanthomonas spadix". Biocompx. 1: 23–32.
- Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH (December 1998). "Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics". PMID 9860944.
- Nan J, Brostromer E, Liu XY, Kristensen O, Su XD (2009). "Bioinformatics and structural characterization of a hypothetical protein from Streptococcus mutans: implication of antibiotic resistance". PMID 19798411.
- Hernández S, Gómez A, Cedano J, Querol E (October 2009). "Bioinformatics annotation of the hypothetical proteins found by omics techniques can help to disclose additional virulence factors". S2CID 9561434.
- Dilip Gore (2009). "In silico Prediction of Structure and Enzymatic Activity for Hypothetical Proteins of Shigellaflexneri. Biofrontiers". Biofrontiers. 1 (2): 1–10.
- Dilip gore; Alankar raut (2009). "Computational Functionand Structural Annotations for Hypothetical proteins of Bacillus anthracis". Biofrontiers. 1 (1): 27–36.
- Dogra Pranay; Dilip Gore (2010). "Prediction of Enzymatic Function and Structure of H. influenzae Hypothetical Proteins - An In silico Approach". International Journal of Soft Computing and Bioinformatics. 1 (in press).
- D G Gore; A P Denge; N M Amrute (2010). "Homology Modeling and Enzyme Function Prediction in the Hypothetical Proteins of Helicobacter pylori - an Insilico Approach". Biomirror. 1: 1–5.
External links
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