Multifocal multiphoton microscopy
 | This article may be too technical for most readers to understand. (July 2008) |
Multifocal multiphoton microscopy is a
CCD camera in the same way as in a conventional microscope. The image rate is determined by the camera frame rate
, depending
on the readout rate and the number of pixels and may range well above 30 images/s.[2]
By exploiting specific properties of pulsed-mode multiphoton excitation the conflict between the density of the foci, i.e. the degree of parallelization, and the axial sectioning has been resolved.time-multiplexing (TMX). Moreover, with a high degree of time multiplicity, the interfocal distance can be reduced to such an extent that lateral scanning becomes obsolete. In this case axial scanning is sufficient to record a 3D-image.
References
- .
- ^ "Stefan Hell Labs" (PDF).
- PMID 18087301.
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External links
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