Myc-tag
A myc tag is a polypeptide
c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography
, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an
Western blotting
.
The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): EQKLISEEDL and Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. The tag is approximately 1202 daltons in atomic mass and has 10 amino acids.
It can be fused to the
secretory pathway.[2]
A monoclonal antibody against the myc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma Bank.[3]
See also
- Protein tag
- Polyhistidine-tag
- SpyTag
- Flag-tag
References
- PMID 3915782.
- ^ "Lentiviral Vector pLV-C-Myc". sinobiological.com. SinoBiological. Retrieved 1 November 2021.
- ^ "Source for 9E10 anti-myc hybridoma". 2009-09-22.