PSF Lab
 PSF Lab on Windows 7 | |
| Developer(s) | Michael J. Nasse, Jörg C. Woehl |
|---|---|
| Stable release | 3.0
/ September 24, 2010 |
Windows, macOS | |
| Type | Computer simulation software |
| License | Freeware for academic and non-commercial use |
| Website | onemolecule |
PSF Lab is a software program that allows the calculation of the illumination
The polarization of the input beam (assumed to be
The following optical parameters can be selected:[2]
- Input beam
- Wavelength
- Gaussian profile filling parameter (0 = constant profile)
- Polarization (linear, circular, elliptic)
- Outputs
- Individual field components
- Squared field components
- Intensity
- Microscope objective
- Numerical aperture
- Optical media
- Refractive index (design and actual)
- Thickness (design and actual)
- Depth (focus position within medium 3)
The program calculates only 2D section of the PSF, but several calculations can be stacked (with a third party program) to obtain the full 3D PSF. Calculations are organized in "sets", each with its own set of parameters. Loops can be set up such that PSF Lab calculates one or several sets, increasing the resolution of the calculated images in each new iteration. The resulting image is displayed in PSF Lab in linear or logarithmic color scale with user-selectable color map, and the intensity, individual field components, or squared field component distributions can be exported into various formats (data formats: .mat, .h5 (HDF5), .txt (ASCII); image formats: .fig, .ai, .bmp, .emf, .eps, .jpg, .pcx, .pdf, .png, .tif).
See also
- Point spread function
- Optical microscope
- Confocal microscopy
- Confocal laser scanning microscopy
References
External links
- Official website
- Molecular Expressions, introduction to deconvolution using PSFs.
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