Resolution (chromatography)

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Example chromatogram showing signal as a function of retention time

In

chromatogram.[1][2][3][4]

Expression

Chromatographic peak resolution is given by

where tR is the retention time and wb is the peak width at baseline. Here compound 1 elutes before compound 2.

If the peaks have the same width

.

Plate number

Two resolved peaks in a chromatogram

The theoretical plate height is given by

where L is the column length and N the number of theoretical plates.[5] The relation between plate number and peak width at the base is given by

.

See also

References

External links