Tissue microarray
Tissue microarrays (also TMAs) consist of
History
The major limitations in molecular
Multi-tissue blocks were first introduced by H. Battifora in 1986 with his so-called “multitumor (sausage) tissue block" and modified in 1990 with its improvement, "the checkerboard tissue block" . In 1998, J. Kononen and collaborators developed the current technique, which uses a novel sampling approach to produce tissues of regular size and shape that can be more densely and precisely arrayed.
Procedure
In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical
One variation is a Frozen tissue array.
Use in research
The use of tissue microarrays in combination with
Immunohistochemistry combined with tissue microarrays has also been used with success in large scale efforts to create a map of protein expression on a more global scale.[6]
See also
References
- ^ "Yale University Core Tissue MicroArray Facility". Archived from the original on 10 May 2009.
- S2CID 21225276.
- PMID 18936473.
- PMID 27624330.
- PMID 28052770.
- PMID 22688270.
- Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest 1986, 55:244-248.
- Battifora H, Mehta P: The checkerboard tissue block. An improved multitissue control block. Lab Invest 1990, 63:722-724.
- Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 1998, 4:844-847.
External links
Media related to Tissue microarray at Wikimedia Commons- National Cancer Institute Tissue Array Research Program
