Cell extrusion
Cell extrusion, discovered in 2001,
Function
Cell extrusion enables the removal of less fit and excess cells from the epithelia and endothelia. Apoptotic epithelial cell extrusion was first discovered as a way to prevent gaps when cell die within an epithelial layer.[1] In vertebrates, most cells extrude out apically into the lumen, however, during fruit fly development, they extrude basally, back into the tissue the epithelia encase where they are engulfed.[3]
During homeostasis, live cell extrusion drives most epithelial cell death when too many cells accumulate. To maintain constant cell numbers, crowding signals some cells to extrude, which then later die through anoikis, or death due to lost of survival signaling, derived from the underlying matrix. Live cell extrusion is essential for maintaining constant cell densities and preventing neoplasms,[5][4][3] as its disruption causes masses to rapidly form.
Triggers
Various factors such as
Signaling controlling extrusion
The rate limiting step for all types of cell extrusion discovered thus far is the production of the bioactive lipid,
Pathologies stemming from defective extrusion
Cancer
Disruption of extrusion not only leads to rapid formation of masses, a number of oncogenic mutations that drive aggressive cancers can hijack extrusion signaling in various ways to cause mass formation, disrupted barrier function, and aberrant Basal Cell Extrusion (BCE).[3] While most cells extrude apically out of the epithelia organs encase, BCE drives cells in the opposite direction—basally, which can enable them to invade through the underlying stroma.[15] Cells transformed with KRas, a driver of pancreatic, and some types of lung and colon cancer, have unregulated autophagy, which degrades S1P and prevents apical extrusion. Instead, cells form masses at sites where they would normally extrude and, at completely separate sites invade by BCE.[15] Importantly, as they invade, BCE also causes the mechanical pinching off of their apical surfaces, which include epithelial determinants, thereby causing the cells to essentially partially de-differentiate.[15] Thus, oncogenic transformation can cause cells to mechanically de-differentiate and invade by hijacking a process that normally drives cell death. Similarly, mutations in APC mis-target S1P, also causing BCE.[12] Moreover, pancreatic cancers and some types of colon and lung cancer vastly down regulate S1P2.[5] Rescue of S1P2 alone is sufficient to reduce orthotopic pancreatic cancer and metastases in a mouse model.[5]
Asthma
Whereas too little extrusion can lead to cancer, too much can drive inflammatory disease. Typically crowding of 1.6-fold causes live cells to extrude during homeostatic turnover. When epithelia experience pathological crowding, as airway epithelial do during an asthma attack, they can extrude at such high rates that it destroys the barrier they should provide.[16] This then mechanically causes the ensuing inflammation and hyper-susceptibility to infections that can follow an asthma attack. Because of this, blocking extrusion during an asthma attack may offer a new way to prevent this inflammatory period and potentially future attacks.