Discontinuous electrophoresis

Edit links
Source: Wikipedia, the free encyclopedia.
DNA bands after electrophoresis

Discontinuous electrophoresis (colloquially disc electrophoresis[a]) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis.[2][1] This method produces high resolution and good band definition. It is widely used technique for separating proteins according to size and charge.[3]

Method

In this method, the gel is divided into two discontinuous parts, resolving and stacking gel, both have different concentrations of polyacrylamide.

homogeneous buffer start to separate based on principles of zone electrophoresis. Now their mobility depends on size as well as charge. pH value rises to 9.5 and net charge increases.[5][6]

See also

References

  1. ^
    S2CID 28591995
    .
  2. S2CID 46152998
    .
  3. ^ PolyAcrylamide Gel Electrophoresis (PDF). davidson.edu.
  4. ^ "Principle of Disc Electrophoresis". Archived from the original on 29 June 2016. Retrieved 4 July 2016.
  5. ISBN 3110836203
    , 9783110836202
  6. ^ "EXPERIMENTAL TECHNIQUES - Electrophoresis". Retrieved 4 July 2016.
  1. ^ Ornstein derived the name disc electrophoresis from: "the dependence of the new technique on discontinuities in the electrophoretic matrix and, coincidentally, from the discoid shape of the separated zones of ions in the standard form of our technique."[1]

External links

Retrieved from "https://en.wikipedia.org/w/index.php?title=Discontinuous_electrophoresis&oldid=1203394017"