Donkey orchid symptomless virus
This article relies largely or entirely on a single source. (April 2019) |
Donkey orchid symptomless virus | |
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Virus classification | |
(unranked): | Virus |
Realm: | Riboviria |
Kingdom: | Orthornavirae |
Phylum: | Kitrinoviricota |
Class: | Alsuviricetes |
Order: | Tymovirales |
Family: | Alphaflexiviridae |
Genus: | Platypuvirus |
Species: | Donkey orchid symptomless virus
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Donkey orchid symptomless virus (DOSV) was first discovered in
Experimental data
Throughout the years 2011-2013, three researchers conducted a study on common
So far, it has only been looked at in the Australian terrestrial orchid Diuris longifolia, commonly named ‘common donkey orchid’ and another terrestrial orchid, Caladenia latifolia (pink fairy orchid). The plants were growing in a leftover patch of forest in Perth. The virus was identified in 3 plants: 2 common donkey orchids (of which 264 plants were tested) and one plant from the group Caladenia latifolia (pink fairy orchid) in which 129 were tested. The donkey orchid has ear-like petals which is where its name originated from.[1]
DOSV most likely evolved in
As of now, DOSV is more uncommon than other plant viruses because it was only found in 2 common donkey orchids out of the 294 that were tested. The uncommonness of this plant virus could be due to the small number of tested plants in this study, the specific area in which the plants were tested from, or that the two orchid species infected by DOSV have not been studied enough. The common donkey orchid is not threatened which could mean a multitude of things, including that the virus itself has a poor method of entry, replication etc. The rarity of the virus could also be due to the inefficient transmission from plant to plant or the unknown
During the experiment that was conducted in 2011, 2012, and 2013, it was proved that
In order to assess how DOSV is scattered among wild orchid plants in Western Australia, RT-PCR was used to analyze the RNA taken from the leaves of common donkey orchids and the “two primer pairs that were used were DOSV8F5200 and DOSV8R6100; DOSV10F6800 and DOSV10R7700).”[1] Therefore, researchers were able to observe if the virus was in the RNA of the common donkey orchids that were being tested.[1]
Viral classification structure
Donkey orchid symptomless virus is a
The movement proteins of the virus seem to be closely related the Furovirus, which is a virus in the family Virgaviridae. This virus also uses plants as its host and has a bipartite genome, just like DOSV.
Genome
The virus has a bipartite genome which means that the genome is segmented into two parts instead of just one part and the genome is 7.8 kb in size.[citation needed]
The RNA from the plants was previously extracted in order to see if the RNA contains the virus. From here, researchers were able to sequence the genome of the virus. The results from the sequencing concluded that the genome contains “7 open reading frames along with a 5’ untranslated region and a 3’ UTR” (untranslated region).[1] The 5’UTR and the 3’UTR (146-147 nt in length) do not share any identity with any other known virus, so these regions of the genome are specific to DOSV.[1]
Donkey orchid symptomless virus also has a putative
Entry into cell
There has not been any research conducted on the virus’s entry into plant cells, but since it is closely related to Furovirus, the virus could possibly have a similar way of entry. Furovirus enters its way into a plant cell by penetrating the
Replication and transcription
There is not much information on the replication and transcription of Donkey orchid symptomless virus because there has not been much research conducted. However, as mentioned before, DOSV is closely related to Furovirus which carries out transcription and replication by using the plant cell’s own machinery.
The replicase in DOSV shares similar identity with plant viruses of the family, Alphaflexiviridae, which are also (+)ssRNA viruses that use plants as their host. Viruses in the Alphaflexiviridae family enter the plant cells and replicate in the same fashion as the Furovirus.
Another virus that is in Alphaflexiviridae and is very similar to DOSV is the
Modulation of host processes/interaction with host
There has not been much research conducted on the exact interaction with the host or how the host processes are affected. This could be due to the fact that plant viruses are not as heavily studied as animal viruses. However, researchers have found that the virus causes the leaves to become necrotic. The plant itself does not necessarily exhibit visible symptoms on the outside, but it does on the inside.[1]
Associated disease(s)
When the virus replicates in the plant it can cause infection which has led to the following visible symptoms: leaf distortion, mosaic patterns on the leaves, and stunting.
Tropism
Though research has not been conducted on the reaction of the common donkey orchid to DOSV, researchers have noticed that most of the orchids in the Western Australian region seem to be healthy. This could be due to the vector of the virus (which is not known) to be unsuccessful when infecting the orchids, the type of environment the orchids are located in or it could be due to survival of the fittest among the orchid population.[1]
References
- ^ PMID 24223974.